##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547255_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1190132
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.381429118786823	31.0	31.0	33.0	30.0	34.0
2	31.79479251041061	31.0	31.0	34.0	30.0	34.0
3	31.926556886126917	31.0	31.0	34.0	30.0	34.0
4	35.60639660138539	37.0	35.0	37.0	33.0	37.0
5	35.47494899725409	37.0	35.0	37.0	33.0	37.0
6	35.52263110310453	37.0	35.0	37.0	33.0	37.0
7	35.94841496573489	37.0	35.0	37.0	35.0	37.0
8	35.931126967428824	37.0	35.0	37.0	35.0	37.0
9	37.61243542733075	39.0	37.0	39.0	35.0	39.0
10	37.126049043299396	39.0	37.0	39.0	33.0	39.0
11	36.89960441362807	39.0	37.0	39.0	33.0	39.0
12	36.46061781382233	39.0	35.0	39.0	32.0	39.0
13	36.35953658921868	39.0	35.0	39.0	32.0	39.0
14	37.39218422830409	39.0	35.0	41.0	32.0	41.0
15	37.54297254422198	39.0	35.0	41.0	32.0	41.0
16	37.59963852749107	39.0	35.0	41.0	33.0	41.0
17	37.50193760019897	39.0	35.0	41.0	32.0	41.0
18	37.42527467541416	39.0	36.0	41.0	32.0	41.0
19	37.36932373887939	39.0	35.0	41.0	32.0	41.0
20	37.18178487764382	39.0	35.0	41.0	32.0	41.0
21	37.05409063868546	39.0	35.0	41.0	32.0	41.0
22	37.026493699858506	39.0	35.0	41.0	32.0	41.0
23	36.94090487441729	38.0	35.0	41.0	32.0	41.0
24	36.837199571140005	38.0	35.0	41.0	32.0	41.0
25	36.641627987483744	38.0	35.0	40.0	31.0	41.0
26	36.56366184591289	38.0	35.0	40.0	31.0	41.0
27	36.417983887501556	38.0	35.0	40.0	31.0	41.0
28	36.36857004097025	38.0	35.0	40.0	31.0	41.0
29	36.28830499474008	38.0	35.0	40.0	30.0	41.0
30	36.23688296760359	38.0	35.0	40.0	30.0	41.0
31	36.1041145015847	38.0	35.0	40.0	30.0	41.0
32	35.895556963429264	38.0	35.0	40.0	30.0	41.0
33	35.743982180127915	38.0	35.0	40.0	29.0	41.0
34	35.623646788759565	38.0	35.0	40.0	28.0	41.0
35	35.399352340748756	38.0	35.0	40.0	27.0	41.0
36	35.2621557944833	38.0	35.0	40.0	26.0	41.0
37	35.20069118383507	38.0	34.0	40.0	26.0	41.0
38	35.11102045823488	38.0	34.0	40.0	26.0	41.0
39	35.01853827978745	38.0	34.0	40.0	25.0	41.0
40	34.912339975733786	38.0	34.0	40.0	24.0	41.0
41	34.89710132993651	38.0	34.0	40.0	24.0	41.0
42	34.706484658844566	37.0	34.0	40.0	24.0	41.0
43	34.65228226784928	37.0	34.0	40.0	24.0	41.0
44	34.398913733938755	37.0	34.0	40.0	23.0	41.0
45	34.23876511176911	37.0	33.0	40.0	23.0	41.0
46	34.259598935244156	37.0	34.0	40.0	23.0	41.0
47	34.13821744142667	36.0	33.0	40.0	23.0	41.0
48	34.101573606961246	36.0	33.0	40.0	23.0	41.0
49	34.03419536656438	36.0	33.0	40.0	23.0	41.0
50	33.471615753546665	35.0	33.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	6.0
14	7.0
15	24.0
16	75.0
17	219.0
18	503.0
19	986.0
20	1698.0
21	2795.0
22	4352.0
23	6541.0
24	9745.0
25	14243.0
26	19160.0
27	22204.0
28	23680.0
29	26008.0
30	29657.0
31	35520.0
32	43672.0
33	55114.0
34	87845.0
35	141378.0
36	92975.0
37	117778.0
38	166883.0
39	287058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.910493962014296	26.10861652320919	27.950260979454377	14.030628535322132
2	32.094003018152605	26.833830196986554	27.950260979454377	13.121905805406458
3	28.363240380058684	26.058033898760808	32.13341041161821	13.4453153095623
4	23.89323201123909	29.72829904582013	31.2725815287716	15.105887414169183
5	22.518846648943143	32.73519239882635	30.005663237355186	14.740297714875325
6	19.161571993694817	41.245004755774985	27.96093206467854	11.632491185851654
7	83.46435521437958	4.115677924801619	9.74505348986499	2.6749133709538104
8	81.71169248453113	3.7352159256284176	8.785328014035418	5.767763575805037
9	75.40844208877671	5.5666094181149655	13.809560620166502	5.215387872941825
10	40.32956008241103	24.082622767894655	18.41745285396914	17.17036429572518
11	31.879993143617686	24.185720575532798	26.674352088675878	17.259934192173638
12	28.491629499921018	20.081974100351893	30.297563631597168	21.12883276812992
13	21.061361260767715	28.91015450387016	32.60587901173987	17.422605223622252
14	16.78360047456921	32.18516937617004	31.00958549135726	20.021644657903494
15	15.385940383083557	23.453532885427837	42.68375272658831	18.476774004900296
16	16.604544705965388	21.112952176733337	40.04202895141043	22.24047416589084
17	15.733380835067035	23.930622821670198	31.23342620818531	29.102570135077453
18	19.30617780212615	23.162052612651372	35.00813355157243	22.52363603365005
19	23.213895601496304	28.809661449318224	27.54618815391906	20.430254795266407
20	24.468882443292003	24.686925483895905	26.966504555797172	23.87768751701492
21	20.18070264474865	31.021937062443495	27.950429028040585	20.846931264767267
22	20.190617511334878	25.77319154513953	26.191884597674882	27.84430634585071
23	18.52651638641764	30.662481136546198	24.811281437689264	25.999721039346895
24	19.242907509419123	25.53657913575973	33.580896908914305	21.63961644590684
25	19.781839325385757	26.196673982381785	30.354700150907632	23.66678654132483
26	17.83449230841621	28.82503789495619	29.818457112320313	23.522012684307285
27	20.477224375111334	27.0065001193145	27.67020801054001	24.846067495034163
28	19.00763948872898	26.753250899900177	33.76096096903537	20.478148642335473
29	18.990498532935842	23.49907405229	32.48152305794652	25.028904356827645
30	21.367377736251107	26.329852486951026	30.96740529621924	21.335364480578626
31	24.922529601758463	24.75851418162019	26.36724329738214	23.95171291923921
32	25.978714966070992	23.81618173446307	28.57195672412808	21.63314657533786
33	22.580184382908786	23.77349739356643	29.74779268182017	23.89852554170462
34	21.043043964871124	23.088699404771905	29.725862341320124	26.142394289036847
35	18.76296074721123	22.32433041040826	32.420689469739486	26.492019372641018
36	25.020081806051763	21.798170286993376	28.100916536989175	25.080831369965683
37	20.221202354024594	24.90236377141359	30.696006829494543	24.180427045067272
38	20.246997812007407	25.371723472690423	27.03607667048697	27.3452020448152
39	19.746297049402923	24.315454084084788	28.261907082575714	27.67634178393657
40	21.753469363062248	21.720615864458733	29.771487532475387	26.75442724000363
41	17.680559803450375	21.4762732201134	29.986421674234453	30.856745302201773
42	22.272571445856425	23.588055778686734	27.84909573055762	26.290277044899224
43	21.274110770906084	22.58211694165017	31.226872313323227	24.91689997412052
44	22.599005824563996	25.293076734345433	28.372146955127665	23.735770485962902
45	19.09578097219468	26.39295473107185	28.73815677588705	25.773107520846427
46	23.115167057099548	24.573240615326704	28.123014926075427	24.18857740149832
47	21.292932212561297	23.618724645669555	29.521095139026592	25.567248002742556
48	21.095055002302267	21.948657795941962	30.144303320976164	26.81198388077961
49	22.1582984072355	20.451765014300936	30.38511694501114	27.004819633452428
50	19.605136236988837	22.83847505990932	31.54238353392733	26.014005169174514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1598.0
1	1534.0
2	1470.0
3	6243.0
4	11016.0
5	8088.0
6	5160.0
7	5779.5
8	6399.0
9	7181.0
10	7963.0
11	8357.0
12	8751.0
13	8721.0
14	8691.0
15	8489.0
16	8287.0
17	8077.0
18	7867.0
19	7459.5
20	7052.0
21	7032.0
22	7012.0
23	6964.0
24	6916.0
25	6930.5
26	6945.0
27	7272.0
28	7599.0
29	8619.5
30	9640.0
31	11394.0
32	13148.0
33	15251.0
34	17354.0
35	20085.0
36	22816.0
37	24311.0
38	25806.0
39	32027.5
40	38249.0
41	48725.0
42	59201.0
43	70565.5
44	81930.0
45	97365.5
46	112801.0
47	127980.0
48	143159.0
49	143355.5
50	143552.0
51	119536.5
52	95521.0
53	80976.5
54	66432.0
55	59594.5
56	52757.0
57	48111.0
58	43465.0
59	41076.0
60	38687.0
61	36397.0
62	34107.0
63	31117.0
64	28127.0
65	24091.5
66	20056.0
67	17002.5
68	13949.0
69	11901.5
70	9854.0
71	8638.5
72	7423.0
73	5978.5
74	4534.0
75	3258.0
76	1982.0
77	1679.5
78	1377.0
79	1111.5
80	846.0
81	600.5
82	355.0
83	274.5
84	194.0
85	119.0
86	44.0
87	34.5
88	25.0
89	15.0
90	5.0
91	5.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1190132.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.601471602518494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14357014453131	25.475953829863496
2	7.993279354046534	5.211853397438909
3	3.0106924420483896	2.944590124600728
4	1.6389368581760173	2.137270137605852
5	1.0946617737348274	1.7843792365389248
6	0.8017133756522838	1.5682221509812304
7	0.6446006931641937	1.471045183520934
8	0.5366098459376715	1.3995416523175055
9	0.4347909638655713	1.275734273534556
>10	4.995277735650718	37.095666966932015
>50	0.6333386402777486	13.291975361180668
>100	0.06682953076123474	3.5543880132057346
>500	0.002331262699180437	0.5244179033288572
>1k	0.0031083502655739155	1.6848086295191675
>5k	2.590291887978263E-4	0.5801531394314411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6870	0.577246893621884	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	2947	0.24761959177637438	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	2341	0.19670087015557935	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	2049	0.17216577656932172	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	1743	0.14645434287961337	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	1645	0.13821996215545837	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1587	0.1333465531554483	No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	1587	0.1333465531554483	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	1350	0.11343279568988986	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1303	0.10948365391401964	No Hit
AAGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	1282	0.10771914375884356	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2617356730177829	0.0
2	0.0	0.0	0.0	0.841167198260361	0.0
3	0.0	0.0	0.0	1.2575075705888086	0.0
4	0.0	0.0	0.0	1.9678489444868301	0.0
5	0.0	0.0	0.0	3.118981760006453	0.0
6	0.0	0.0	0.0	5.22849566266599	0.0
7	0.0	0.0	0.0	6.270817018616422	0.0
8	0.0	0.0	0.0	9.402402422588418	0.0
9	0.0	0.0	0.0	11.058101118195292	0.0
10	0.0	0.0	0.0	13.52673484957971	0.0
11	0.0	0.0	0.0	15.009595574272433	0.0
12	0.0	0.0	0.0	16.302897493723385	0.0
13	0.0	0.0	0.0	16.9876114582248	0.0
14	0.0	0.0	0.0	17.288502451828872	0.0
15	0.0	0.0	0.0	17.591494052760535	0.0
16	0.0	0.0	0.0	18.161682905761715	0.0
17	0.0	0.0	0.0	18.753718074969836	0.0
18	0.0	0.0	0.0	19.668154456816556	0.0
19	0.0	0.0	0.0	20.02761038271385	0.0
20	0.0	0.0	0.0	20.498818618438964	0.0
21	0.0	0.0	0.0	20.95246577690542	0.0
22	0.0	0.0	0.0	21.412414757354647	0.0
23	0.0	0.0	0.0	21.944540605579885	0.0
24	0.0	0.0	0.0	22.34222758483933	0.0
25	0.0	0.0	0.0	22.648580157495136	0.0
26	0.0	0.0	0.0	22.938127871530217	0.0
27	0.0	0.0	0.0	23.271368217979184	0.0
28	0.0	0.0	0.0	23.57217518729015	0.0
29	0.0	0.0	0.0	23.90071017332531	0.0
30	0.0	0.0	0.0	24.312177136653748	0.0
31	0.0	0.0	0.0	24.644073094413056	0.0
32	0.0	0.0	0.0	24.93832616886194	0.0
33	0.0	0.0	0.0	25.244594717224643	0.0
34	0.0	0.0	0.0	25.559181670604605	0.0
35	0.0	0.0	0.0	25.907210292639807	0.0
36	8.402429310362212E-5	0.0	0.0	26.21919249293356	0.0
37	8.402429310362212E-5	0.0	0.0	26.538568831020424	0.0
38	8.402429310362212E-5	0.0	0.0	26.85836529057281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	55	1.8189894E-12	44.000004	1
TATAACG	55	1.8189894E-12	44.000004	1
ACGCATT	20	7.856596E-4	44.0	15
CCGGATA	20	7.856596E-4	44.0	20
TATCTCG	20	7.856596E-4	44.0	1
CTCGCTA	20	7.856596E-4	44.0	12
GCGTATG	35	1.4461511E-7	44.0	1
TCACGAC	130	0.0	43.999996	25
CGTTTTT	5545	0.0	42.651035	1
CAACCCG	80	0.0	41.25	23
CGAATAT	75	0.0	41.066666	14
ATAACGG	145	0.0	40.965515	2
CGGTCTA	135	0.0	40.74074	31
ATAGCGG	190	0.0	40.526314	2
CGTTTAT	115	0.0	40.173916	39
TCGTTTA	110	0.0	40.000004	38
CGCATCG	110	0.0	40.000004	21
TACGGGA	265	0.0	39.849056	4
GCAATAG	100	0.0	39.6	1
TAGGGAC	935	0.0	39.294117	5
>>END_MODULE