SRR1547254_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1547254_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1004244
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10762	1.0716519092969439	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	1798	0.1790401535881718	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	1398	0.13920919617144836	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	1327	0.13213920122997996	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1243	0.12377470017246806	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	1212	0.12068780097267198	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	1086	0.10814104938640409	Illumina PCR Primer Index 5 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1081	0.10764316241869505	Illumina PCR Primer Index 5 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1054	0.10495457279306622	Illumina PCR Primer Index 5 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	20	7.856004E-4	44.000004	1
CGCCTAT	20	7.856004E-4	44.000004	35
CGTTTTT	9070	0.0	42.811466	1
CGTATGG	60	3.6379788E-12	40.333332	2
ATCGTAG	45	2.3504981E-8	39.11111	1
ACGGGAA	410	0.0	38.634148	5
TTAACGG	40	4.123849E-7	38.500004	2
CGTTCTG	150	0.0	38.13333	1
AGGGCGA	1125	0.0	38.13333	6
GTTTTTT	10190	0.0	38.041218	2
CGAGGGA	685	0.0	37.897808	4
CGGGAAC	280	0.0	37.714287	6
TCGACGG	35	7.289287E-6	37.714287	2
AAGGGCG	445	0.0	37.573032	5
GGGCGAT	2285	0.0	37.164112	7
ACGGGCG	155	0.0	36.903225	5
GCGATAA	90	0.0	36.666668	9
TACGGTT	210	0.0	36.666664	33
CTAACGG	60	1.9826984E-10	36.666664	2
GTGTACG	30	1.3009783E-4	36.666664	1