##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547254_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1004244
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42526119150326	31.0	31.0	33.0	30.0	34.0
2	31.844738927989614	31.0	31.0	34.0	30.0	34.0
3	31.93669068473399	33.0	31.0	34.0	30.0	34.0
4	35.63358606075814	37.0	35.0	37.0	33.0	37.0
5	35.5122749053019	37.0	35.0	37.0	33.0	37.0
6	35.56472132270643	37.0	35.0	37.0	33.0	37.0
7	36.00673541489917	37.0	35.0	37.0	35.0	37.0
8	36.0083585264139	37.0	35.0	37.0	35.0	37.0
9	37.66545082669152	39.0	37.0	39.0	35.0	39.0
10	37.13696073862528	39.0	37.0	39.0	33.0	39.0
11	36.95105671530027	39.0	37.0	39.0	33.0	39.0
12	36.69722198987497	39.0	35.0	39.0	32.0	39.0
13	36.67354248569073	39.0	35.0	39.0	32.0	39.0
14	37.7884159626545	40.0	36.0	41.0	33.0	41.0
15	37.86352619482915	40.0	36.0	41.0	33.0	41.0
16	37.86485156993719	40.0	36.0	41.0	33.0	41.0
17	37.73948064414624	40.0	36.0	41.0	33.0	41.0
18	37.57937513194005	39.0	36.0	41.0	32.0	41.0
19	37.47569216246251	39.0	36.0	41.0	32.0	41.0
20	37.261879583049534	39.0	35.0	41.0	32.0	41.0
21	37.130765033199104	39.0	35.0	41.0	32.0	41.0
22	37.08630671430449	39.0	35.0	41.0	32.0	41.0
23	36.98532229219194	39.0	35.0	41.0	32.0	41.0
24	36.88827416444609	38.0	35.0	41.0	32.0	41.0
25	36.718889034935735	38.0	35.0	41.0	31.0	41.0
26	36.63726146235377	38.0	35.0	40.0	31.0	41.0
27	36.468233815686226	38.0	35.0	40.0	31.0	41.0
28	36.409709194179904	38.0	35.0	40.0	31.0	41.0
29	36.32536216298031	38.0	35.0	40.0	30.0	41.0
30	36.23888716288074	38.0	35.0	40.0	30.0	41.0
31	36.09612902840345	38.0	35.0	40.0	30.0	41.0
32	35.785885701084595	38.0	35.0	40.0	28.0	41.0
33	35.50427983637443	38.0	35.0	41.0	26.0	41.0
34	35.25795324642218	38.0	35.0	41.0	24.0	41.0
35	34.94918565607561	38.0	34.0	41.0	23.0	41.0
36	34.80172049820562	38.0	34.0	40.0	22.0	41.0
37	34.723474573908334	38.0	34.0	40.0	22.0	41.0
38	34.60504717976906	38.0	34.0	40.0	22.0	41.0
39	34.54127482962308	38.0	34.0	40.0	21.0	41.0
40	34.51438992914073	38.0	34.0	40.0	21.0	41.0
41	34.503003254189224	38.0	34.0	40.0	21.0	41.0
42	34.346439709871305	38.0	34.0	40.0	20.0	41.0
43	34.32781574995718	38.0	33.0	40.0	20.0	41.0
44	34.0910217038887	38.0	33.0	40.0	18.0	41.0
45	33.90799447146311	37.0	33.0	40.0	18.0	41.0
46	33.94412214561402	37.0	33.0	40.0	20.0	41.0
47	33.80778675302018	37.0	33.0	40.0	18.0	41.0
48	33.762584590995814	37.0	33.0	40.0	18.0	41.0
49	33.680960005735656	37.0	33.0	40.0	18.0	41.0
50	33.09423506637829	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	2.0
14	8.0
15	18.0
16	62.0
17	162.0
18	464.0
19	875.0
20	1586.0
21	2566.0
22	3982.0
23	5910.0
24	9030.0
25	14168.0
26	21192.0
27	25039.0
28	24645.0
29	24154.0
30	25448.0
31	29013.0
32	34954.0
33	43377.0
34	64003.0
35	91758.0
36	77766.0
37	96581.0
38	147039.0
39	260436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.10215843958241	23.306487268034463	26.18258112570252	18.408773166680607
2	36.38229354618997	24.408809014542282	25.855270233130597	13.353627206137153
3	27.566607318540115	24.953995244183684	34.45905576732348	13.020341669952721
4	23.432751403045472	27.15604972496724	34.719251496648226	14.691947375339062
5	21.672920126981094	30.496572546114294	33.57112414911117	14.259383177793444
6	19.3157240670594	38.82094391402886	30.791520785785124	11.07181123312661
7	81.61532456255651	3.107910029833387	12.932514408848844	2.344250998761257
8	80.80974344880329	2.697551591047594	12.534304412075153	3.958400548073974
9	74.09265079004705	5.3342613946411435	16.02648360358638	4.5466042117254375
10	32.27890831311912	32.05127439148255	21.44538578273806	14.22443151266027
11	27.092021460919856	25.655617559079268	32.27711592003537	14.975245059965506
12	24.875627835466283	22.229756911666886	33.51864686271464	19.37596839015219
13	21.023177634120792	26.246111502782192	35.916868808775554	16.81384205432146
14	18.180043893715073	29.09103763627166	34.026790301958485	18.702128168054777
15	17.58576600905756	24.54463257933331	40.73312860221221	17.13647280939692
16	18.98433050135226	23.90265712316927	39.44111192100724	17.671900454471224
17	18.596078243932748	24.617025344438204	34.57854863957365	22.208347772055397
18	21.179713296768515	23.34263386189014	35.09107348413334	20.38657935720801
19	22.444346194749485	27.87061710102326	31.067947630257187	18.61708907397007
20	25.00716957233501	25.15295087648022	30.089998048283086	19.749881502901683
21	22.457789142877626	28.415703753271117	30.861324538657936	18.26518256519332
22	21.620343263191018	24.326458510083206	30.7347616714663	23.318436555259478
23	19.714133218620177	29.438363584945492	30.14197744771191	20.705525748722422
24	19.622721171348793	25.40069943161224	35.13190021548548	19.844679181553488
25	20.5975838541231	26.079916832960915	32.83026834116012	20.492230971755866
26	19.45055185791501	28.15212239256595	32.13661221774788	20.260713531771163
27	20.805999338806107	26.107599348365536	30.83284540410498	22.253555908723378
28	19.37517177100386	27.7825906851323	33.66910830435631	19.17312923950753
29	19.789314150744243	24.793775217974915	32.33656362397983	23.080347007301015
30	21.12046474761114	25.47627867331047	31.539048279103483	21.86420829997491
31	22.815969027447515	26.55061917223304	28.200019118859558	22.433392681459885
32	22.475215186747445	25.643767849247794	28.73295732909532	23.148059634909444
33	20.98563695675553	27.717865379330124	28.289439618260104	23.007058045654244
34	20.051999314907533	28.10581890456901	28.592055317233662	23.250126463289796
35	19.47106480098462	28.904628755561397	29.707222547508373	21.91708389594561
36	22.955377378406045	28.85274893352612	26.539466504156362	21.65240718391148
37	21.12265545026906	29.766769828846375	27.735092268412853	21.37548245247171
38	21.125841926862396	30.671231294386626	27.197473920680633	21.00545285807035
39	21.10463194203799	28.8784399010599	26.491669355256292	23.525258801645816
40	22.9528879435675	27.237105723310272	28.291132433950317	21.518873899171915
41	19.245522004612425	27.11801116063427	28.228597830806056	25.407869003947248
42	22.00859552061053	28.281174694596135	26.549922130478247	23.160307654315087
43	21.327784880965183	27.127271858233655	28.60649403929722	22.938449221503937
44	22.42204085859612	28.432731487566766	27.61649559270456	21.52873206113255
45	20.59867920545206	27.36117915566336	28.424466563902794	23.615675074981777
46	23.180721019991157	27.505367221511907	26.782435344398376	22.531476414098567
47	22.021441004377422	26.48171161590211	28.627405291941006	22.869442087779465
48	22.33142543047307	25.11351822863766	28.574131386396136	23.980924954493133
49	21.84996873269843	24.719988369360436	28.37408040277064	25.055962495170498
50	20.391657804278644	25.824401241132634	30.567869959890224	23.216070994698498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	643.0
1	714.0
2	785.0
3	9271.0
4	17757.0
5	12972.0
6	8187.0
7	8373.0
8	8559.0
9	8906.0
10	9253.0
11	9390.5
12	9528.0
13	9446.5
14	9365.0
15	8992.0
16	8619.0
17	8253.0
18	7887.0
19	7613.0
20	7339.0
21	7314.5
22	7290.0
23	7304.5
24	7319.0
25	7685.0
26	8051.0
27	8851.5
28	9652.0
29	11417.5
30	13183.0
31	14824.5
32	16466.0
33	19015.0
34	21564.0
35	23914.0
36	26264.0
37	28979.0
38	31694.0
39	36420.0
40	41146.0
41	46893.5
42	52641.0
43	60433.5
44	68226.0
45	76412.0
46	84598.0
47	94138.0
48	103678.0
49	103367.0
50	103056.0
51	89863.0
52	76670.0
53	67696.5
54	58723.0
55	51344.0
56	43965.0
57	38965.0
58	33965.0
59	32124.5
60	30284.0
61	27414.0
62	24544.0
63	20899.0
64	17254.0
65	14150.0
66	11046.0
67	9146.0
68	7246.0
69	6388.5
70	5531.0
71	4981.5
72	4432.0
73	3891.0
74	3350.0
75	2787.5
76	2225.0
77	1714.0
78	1203.0
79	891.5
80	580.0
81	443.5
82	307.0
83	217.5
84	128.0
85	82.5
86	37.0
87	19.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1004244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.470136667038844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11236420731396	30.398076448543932
2	8.192903785877988	6.631358718287643
3	3.7604144709585667	4.565534626932114
4	2.3303007039601895	3.772303518582629
5	1.6594844600276142	3.3579781447072357
6	1.298908827690781	3.154021066480148
7	0.9922952744718131	2.8110827760351778
8	0.814015563727049	2.6354656890504273
9	0.6713085701690812	2.445115462244744
>10	5.079576235897682	34.72775795579095
>50	0.06663148505576723	1.7699059286257128
>100	0.018328803420519756	1.3246594150057718
>500	0.0012384326531991942	0.30562894716270317
>1k	0.0019814922451187105	1.0223383509716004
>5k	0.0	0.0
>10k+	2.476865306398388E-4	1.0787729515792102
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10762	1.0716519092969439	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	1798	0.1790401535881718	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	1398	0.13920919617144836	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	1327	0.13213920122997996	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1243	0.12377470017246806	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	1212	0.12068780097267198	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	1086	0.10814104938640409	Illumina PCR Primer Index 5 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1081	0.10764316241869505	Illumina PCR Primer Index 5 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	1054	0.10495457279306622	Illumina PCR Primer Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13423032649435795	0.0
2	0.0	0.0	0.0	0.44282066908042267	0.0
3	0.0	0.0	0.0	0.8011001310438499	0.0
4	0.0	0.0	0.0	1.1988122408498334	0.0
5	0.0	0.0	0.0	1.971433237340726	0.0
6	0.0	0.0	0.0	3.5869768701630282	0.0
7	0.0	0.0	0.0	4.522307327701236	0.0
8	0.0	0.0	0.0	6.7725572669590255	0.0
9	0.0	0.0	0.0	8.058798459338567	0.0
10	0.0	0.0	0.0	9.732594867382828	0.0
11	0.0	0.0	0.0	10.5151736032279	0.0
12	0.0	0.0	0.0	11.280226717809615	0.0
13	0.0	0.0	0.0	11.814857743735587	0.0
14	0.0	0.0	0.0	12.091284588207646	0.0
15	0.0	0.0	0.0	12.310354853999625	0.0
16	0.0	0.0	0.0	12.666543190698675	0.0
17	0.0	0.0	0.0	13.021735753462305	0.0
18	0.0	0.0	0.0	13.618901382532531	0.0
19	0.0	0.0	0.0	13.872226271702893	0.0
20	0.0	0.0	0.0	14.204914343526076	0.0
21	0.0	0.0	0.0	14.4903031534169	0.0
22	0.0	0.0	0.0	14.812037711950483	0.0
23	0.0	0.0	0.0	15.171611680029953	0.0
24	0.0	0.0	0.0	15.453316126359729	0.0
25	0.0	0.0	0.0	15.708632563400926	0.0
26	0.0	0.0	0.0	15.946622533965849	0.0
27	0.0	0.0	0.0	16.228924444656876	0.0
28	0.0	0.0	0.0	16.49728552025205	0.0
29	0.0	0.0	0.0	16.769032227227648	0.0
30	0.0	0.0	0.0	17.115262824572515	0.0
31	0.0	0.0	0.0	17.41499077913336	0.0
32	0.0	0.0	0.0	17.734036748041312	0.0
33	0.0	0.0	0.0	18.031673577337777	0.0
34	0.0	0.0	0.0	18.325626043073196	0.0
35	0.0	0.0	0.0	18.61698949657653	0.0
36	0.0	0.0	0.0	18.900984222957767	0.0
37	0.0	0.0	0.0	19.18796627114526	0.0
38	0.0	0.0	0.0	19.475844515874627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	20	7.856004E-4	44.000004	1
CGCCTAT	20	7.856004E-4	44.000004	35
CGTTTTT	9070	0.0	42.811466	1
CGTATGG	60	3.6379788E-12	40.333332	2
ATCGTAG	45	2.3504981E-8	39.11111	1
ACGGGAA	410	0.0	38.634148	5
TTAACGG	40	4.123849E-7	38.500004	2
CGTTCTG	150	0.0	38.13333	1
AGGGCGA	1125	0.0	38.13333	6
GTTTTTT	10190	0.0	38.041218	2
CGAGGGA	685	0.0	37.897808	4
CGGGAAC	280	0.0	37.714287	6
TCGACGG	35	7.289287E-6	37.714287	2
AAGGGCG	445	0.0	37.573032	5
GGGCGAT	2285	0.0	37.164112	7
ACGGGCG	155	0.0	36.903225	5
GCGATAA	90	0.0	36.666668	9
TACGGTT	210	0.0	36.666664	33
CTAACGG	60	1.9826984E-10	36.666664	2
GTGTACG	30	1.3009783E-4	36.666664	1
>>END_MODULE