FastQCFastQC Report
Thu 26 May 2016
SRR1547253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547253_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1491436
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63370.42489251969243064No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33530.22481688788523277No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG29090.19504692122223147No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC25880.17352403991857512No Hit
GAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC25330.16983631882293307No Hit
AAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC22880.15340919757870938No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT20820.13959700583866821No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT20260.13584223526855996No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT16980.11385000764364007No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG16760.11237491920538327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG650.044.0000041
CGTCACT650.044.00000441
AACCGGA254.4434797E-544.017
GTCGATC302.528226E-644.044
GATCGCC207.857237E-444.014
ACGTTAC207.857237E-444.010
CGTCTAG207.857237E-444.038
CTAAGCG551.8189894E-1244.01
TCGATTG600.044.01
ACTTACG351.4464786E-744.01
ACTCGTT254.4434797E-544.010
ACTCGAT207.857237E-444.016
TACTCGA207.857237E-444.015
ATCGGTC207.857237E-444.010
ATACGCC207.857237E-444.023
TAATGCG502.7284841E-1144.01
GTACGAC207.857237E-444.022
TACCGAA207.857237E-444.042
CGTTTTT50350.041.902681
CACGACG4050.041.82716426
Produced by FastQC (version 0.11.5)