##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547253_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1491436
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.364154412257715	31.0	31.0	33.0	30.0	34.0
2	31.77216521526904	31.0	31.0	34.0	30.0	34.0
3	31.92302385083906	31.0	31.0	34.0	30.0	34.0
4	35.58789582657251	37.0	35.0	37.0	33.0	37.0
5	35.44471502632362	37.0	35.0	37.0	33.0	37.0
6	35.50412756564814	37.0	35.0	37.0	33.0	37.0
7	35.937315446321534	37.0	35.0	37.0	35.0	37.0
8	35.92591904714651	37.0	35.0	37.0	35.0	37.0
9	37.60044547670835	39.0	37.0	39.0	35.0	39.0
10	37.111818408567316	39.0	37.0	39.0	33.0	39.0
11	36.83066118827761	39.0	37.0	39.0	32.0	39.0
12	36.17281867944719	38.0	35.0	39.0	32.0	39.0
13	35.97072217647958	38.0	35.0	39.0	32.0	39.0
14	36.95101767692345	39.0	35.0	41.0	32.0	41.0
15	37.1697773152854	39.0	35.0	41.0	32.0	41.0
16	37.28389820280589	39.0	35.0	41.0	32.0	41.0
17	37.188140825352214	39.0	35.0	41.0	32.0	41.0
18	37.13836597748747	38.0	35.0	41.0	32.0	41.0
19	37.110230006517206	38.0	35.0	41.0	32.0	41.0
20	36.93078348651903	38.0	35.0	41.0	32.0	41.0
21	36.76870814436556	38.0	35.0	41.0	32.0	41.0
22	36.722999176632456	38.0	35.0	41.0	31.0	41.0
23	36.644795351594034	38.0	35.0	40.0	31.0	41.0
24	36.5278483287248	38.0	35.0	40.0	31.0	41.0
25	36.35846727583349	38.0	35.0	40.0	31.0	41.0
26	36.228571658455344	38.0	35.0	40.0	31.0	41.0
27	36.08202966805146	38.0	35.0	40.0	30.0	41.0
28	36.033441595884774	37.0	35.0	40.0	30.0	41.0
29	36.02760628012198	37.0	35.0	40.0	30.0	41.0
30	35.95397455874741	37.0	35.0	40.0	30.0	41.0
31	35.87742350325458	37.0	35.0	40.0	30.0	41.0
32	35.74937912186644	38.0	35.0	40.0	30.0	41.0
33	35.67198994794279	38.0	35.0	40.0	29.0	41.0
34	35.6405102196809	38.0	35.0	40.0	29.0	41.0
35	35.51124687884696	38.0	35.0	40.0	29.0	41.0
36	35.35298598129588	37.0	35.0	40.0	27.0	41.0
37	35.29259116717043	37.0	34.0	40.0	27.0	41.0
38	35.20249008338273	37.0	34.0	40.0	27.0	41.0
39	35.142437892071804	37.0	34.0	40.0	27.0	41.0
40	35.00070670146087	37.0	34.0	40.0	26.0	41.0
41	34.997234209178266	37.0	34.0	40.0	26.0	41.0
42	34.81642591435369	37.0	34.0	40.0	26.0	41.0
43	34.77621031006359	36.0	34.0	40.0	26.0	41.0
44	34.5463452672458	36.0	34.0	40.0	24.0	41.0
45	34.388817220450626	36.0	34.0	40.0	24.0	41.0
46	34.4467653992528	36.0	34.0	40.0	24.0	41.0
47	34.31999898084799	36.0	33.0	40.0	24.0	41.0
48	34.308123848425275	36.0	34.0	40.0	24.0	41.0
49	34.24022217513859	36.0	33.0	40.0	24.0	41.0
50	33.55660853030234	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	3.0
14	14.0
15	38.0
16	89.0
17	246.0
18	585.0
19	1187.0
20	2149.0
21	3363.0
22	5251.0
23	7660.0
24	11208.0
25	16169.0
26	21566.0
27	26099.0
28	29097.0
29	32746.0
30	38686.0
31	46492.0
32	57954.0
33	73938.0
34	123609.0
35	215649.0
36	110915.0
37	132637.0
38	192136.0
39	341945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.269120498633534	25.090315642106	27.657707068891995	12.982856790368476
2	30.342032779147075	26.699167781922927	29.17168420233922	13.787115236590775
3	28.906101233978525	25.886259953494484	31.140927267412078	14.06671154511491
4	24.778066239516814	29.910636460431423	30.26385309191947	15.047444208132296
5	22.957471859335566	33.16240187309412	29.6639614438702	14.216164823700112
6	19.960963795965768	41.73695686573209	26.483536672039566	11.818542666262582
7	84.90689509975621	4.10966343845797	8.415245441306231	2.5681960204795917
8	82.93302562094517	3.8743868325560067	7.3181819400899535	5.874405606408857
9	76.60731000190421	5.475260084911455	12.781708366969818	5.135721546214521
10	45.557234772393855	22.69973367948742	16.197476794176886	15.54555475394184
11	38.67252768472801	22.246211034197913	24.056144547939034	15.025116733135047
12	34.58995223395439	19.80024620567024	26.701313365105843	18.908488195269527
13	21.08538348276426	34.406169624442484	29.228206909314245	15.28023998347901
14	16.064718834733775	37.252218667110085	28.555432482520203	18.127630015635937
15	13.98055297042582	23.12355340758839	45.81705148595046	17.078842136035338
16	14.925682362501643	19.815801683746404	43.635395685768614	21.62312026798334
17	15.466369324597235	22.95116920873574	29.89172850863195	31.690732958035074
18	19.52963452672458	23.31953902145315	34.87625348992515	22.274572961897125
19	24.584427357258374	29.645120541545193	25.267192155747882	20.503259945448548
20	26.905881311702277	24.584695555156237	24.63504971048037	23.87437342266111
21	20.33965922775097	31.967043842310368	26.21600926891935	21.47728766101931
22	21.299606553683834	25.429787131328464	24.49779943624802	28.772806878739683
23	18.609179341252325	31.925204970243442	23.235257832049115	26.23035785645512
24	19.189894839604246	25.21918473203007	35.335341241595344	20.255579186770333
25	18.097860048972937	26.05495643125149	31.71433437304718	24.13284914672839
26	17.084340192941568	31.102373819594003	28.531764018033627	23.281521969430806
27	20.05664339602906	29.031148503858027	26.717137041079873	24.195071059033037
28	17.555899146862487	27.95963085241338	34.071458647907114	20.41301135281702
29	17.521033420140053	24.052926173164654	32.09168881534307	26.334351591352227
30	20.09848226809598	28.202551098404488	28.59821004722965	23.100756586269878
31	25.342488715573445	26.848621060508126	23.719019790322886	24.089870433595543
32	25.960886018575387	25.012203004352852	24.732338497930854	24.294572479140907
33	23.420716678422675	27.406606787016003	24.661601302368993	24.51107523219233
34	17.790035911698524	24.765125690944835	29.856862781909516	27.587975615447125
35	17.597067524184745	24.826677108504825	32.25005967403228	25.32619569327816
36	25.037279507803216	23.00018237457055	27.08322717166543	24.879310945960807
37	20.235665492853865	26.757567874183003	31.298493532407694	21.708273100555438
38	19.74955680297378	27.2375080124122	26.686026084927544	26.326909099686475
39	21.939794935887292	26.25174663880985	27.298321885753058	24.510136539549805
40	24.018328644340087	22.19639327466951	27.428330816743056	26.356947264247342
41	18.957099064257534	22.138730726628562	27.355649186421676	31.54852102269222
42	23.449816150341015	25.084281189404038	24.43779015660075	27.028112503654196
43	22.56268455367847	24.235568941610637	28.101306391960502	25.100440112750395
44	22.125991326479983	27.192651913994297	27.5173725188342	23.16398424069152
45	18.577599038778732	29.38255479953548	26.141115005940584	25.898731155745203
46	23.923990033766117	27.001963208612374	26.869071150220325	22.204975607401188
47	21.736366830356786	24.259907900841874	28.10935232889645	25.894372939904898
48	22.375817668341117	21.30430001689647	29.40756425351138	26.912318061251035
49	22.519169444749892	19.4617134090903	31.143005801120534	26.876111345039277
50	20.153932183479544	25.282881732772978	29.75602037231232	24.807165711435154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1406.0
1	1468.0
2	1530.0
3	5764.5
4	9999.0
5	7307.5
6	4616.0
7	5132.5
8	5649.0
9	6443.0
10	7237.0
11	7567.0
12	7897.0
13	7848.0
14	7799.0
15	7532.0
16	7265.0
17	6918.5
18	6572.0
19	6403.0
20	6234.0
21	5870.5
22	5507.0
23	5598.0
24	5689.0
25	5940.5
26	6192.0
27	6706.0
28	7220.0
29	8792.0
30	10364.0
31	12820.0
32	15276.0
33	17476.5
34	19677.0
35	24838.5
36	30000.0
37	31725.0
38	33450.0
39	43491.5
40	53533.0
41	69930.5
42	86328.0
43	105672.5
44	125017.0
45	141831.0
46	158645.0
47	175162.5
48	191680.0
49	186757.0
50	181834.0
51	153890.5
52	125947.0
53	105576.0
54	85205.0
55	74487.0
56	63769.0
57	58242.5
58	52716.0
59	49901.0
60	47086.0
61	40395.0
62	33704.0
63	30633.5
64	27563.0
65	23397.0
66	19231.0
67	16205.0
68	13179.0
69	11453.0
70	9727.0
71	7935.0
72	6143.0
73	5021.5
74	3900.0
75	3612.5
76	3325.0
77	2381.5
78	1438.0
79	1056.0
80	674.0
81	724.0
82	774.0
83	529.0
84	284.0
85	199.5
86	115.0
87	70.0
88	25.0
89	19.0
90	13.0
91	7.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1491436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.86329716838105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94227322028746	20.41707471443824
2	8.514669178268429	4.404348384960225
3	2.8945860351849957	2.245906164223063
4	1.4984967279692336	1.5502426472525979
5	0.9581585264234844	1.2390569351654332
6	0.6731802737526661	1.0446396880774385
7	0.49608832009605824	0.8981335751085106
8	0.388375420875043	0.8035735138389039
9	0.34264240022724385	0.7975675997608105
>10	3.436913028337907	20.46394961050233
>50	1.292788073516304	24.523450140486442
>100	0.5558352596806408	18.951704055618865
>500	0.002084707958454505	0.41165951268522144
>1k	0.003648238927295384	1.8216000833097847
>5k	2.605884948068131E-4	0.42709337457207736
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6337	0.42489251969243064	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3353	0.22481688788523277	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	2909	0.19504692122223147	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2588	0.17352403991857512	No Hit
GAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	2533	0.16983631882293307	No Hit
AAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	2288	0.15340919757870938	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	2082	0.13959700583866821	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	2026	0.13584223526855996	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	1698	0.11385000764364007	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1676	0.11237491920538327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18565999479696077	0.0
2	0.0	0.0	0.0	0.6873912122276785	0.0
3	0.0	0.0	0.0	1.048854929075066	0.0
4	0.0	0.0	0.0	1.8159009169686129	0.0
5	0.0	0.0	0.0	2.955607883945406	0.0
6	0.0	0.0	0.0	4.912178598344147	0.0
7	0.0	0.0	0.0	5.874137408510992	0.0
8	0.0	0.0	0.0	9.215346820111623	0.0
9	0.0	0.0	0.0	10.890980236496906	0.0
10	0.0	0.0	0.0	13.241801860757015	0.0
11	0.0	0.0	0.0	14.579640024781487	0.0
12	6.704947446621914E-5	0.0	0.0	15.781703003011863	0.0
13	6.704947446621914E-5	0.0	0.0	16.331441644160392	0.0
14	6.704947446621914E-5	0.0	0.0	16.564036271083708	0.0
15	6.704947446621914E-5	0.0	0.0	16.793881869553907	0.0
16	6.704947446621914E-5	0.0	0.0	17.29051732692519	0.0
17	6.704947446621914E-5	0.0	0.0	17.805256142402357	0.0
18	6.704947446621914E-5	0.0	0.0	18.609849835996986	0.0
19	6.704947446621914E-5	0.0	0.0	18.929407631302986	0.0
20	1.3409894893243827E-4	0.0	0.0	19.3418289487447	0.0
21	1.3409894893243827E-4	0.0	0.0	19.758005036756522	0.0
22	1.3409894893243827E-4	0.0	0.0	20.14414296020748	0.0
23	1.3409894893243827E-4	0.0	0.0	20.601018079220296	0.0
24	1.3409894893243827E-4	0.0	0.0	20.942903349523547	0.0
25	1.3409894893243827E-4	0.0	0.0	21.205334992584326	0.0
26	1.3409894893243827E-4	0.0	0.0	21.4588490555411	0.0
27	2.011484233986574E-4	0.0	0.0	21.717257730133912	0.0
28	2.011484233986574E-4	0.0	0.0	21.970101298346023	0.0
29	2.011484233986574E-4	0.0	0.0	22.242053966781008	0.0
30	2.011484233986574E-4	0.0	0.0	22.612435263732404	0.0
31	2.6819789786487654E-4	0.0	0.0	22.90564261557318	0.0
32	2.6819789786487654E-4	0.0	0.0	23.159357826953354	0.0
33	2.6819789786487654E-4	0.0	0.0	23.4268181805991	0.0
34	2.6819789786487654E-4	0.0	0.0	23.693406891076787	0.0
35	2.6819789786487654E-4	0.0	0.0	24.005388095768105	0.0
36	2.6819789786487654E-4	0.0	0.0	24.254342794461177	0.0
37	2.6819789786487654E-4	0.0	0.0	24.508862599534943	0.0
38	2.6819789786487654E-4	0.0	0.0	24.780480020597597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	65	0.0	44.000004	1
CGTCACT	65	0.0	44.000004	41
AACCGGA	25	4.4434797E-5	44.0	17
GTCGATC	30	2.528226E-6	44.0	44
GATCGCC	20	7.857237E-4	44.0	14
ACGTTAC	20	7.857237E-4	44.0	10
CGTCTAG	20	7.857237E-4	44.0	38
CTAAGCG	55	1.8189894E-12	44.0	1
TCGATTG	60	0.0	44.0	1
ACTTACG	35	1.4464786E-7	44.0	1
ACTCGTT	25	4.4434797E-5	44.0	10
ACTCGAT	20	7.857237E-4	44.0	16
TACTCGA	20	7.857237E-4	44.0	15
ATCGGTC	20	7.857237E-4	44.0	10
ATACGCC	20	7.857237E-4	44.0	23
TAATGCG	50	2.7284841E-11	44.0	1
GTACGAC	20	7.857237E-4	44.0	22
TACCGAA	20	7.857237E-4	44.0	42
CGTTTTT	5035	0.0	41.90268	1
CACGACG	405	0.0	41.827164	26
>>END_MODULE