FastQCFastQC Report
Thu 26 May 2016
SRR1547252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547252_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1312898
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.503771046951096No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC34820.265214814859951TruSeq Adapter, Index 10 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34060.2594260940301531No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG33800.2574457421673275RNA PCR Primer, Index 35 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG22980.17503263772204694RNA PCR Primer, Index 10 (95% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC17680.13466392667214055No Hit
AAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC16920.12887520584234266TruSeq Adapter, Index 10 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG14740.11227071714634343RNA PCR Primer, Index 35 (95% over 22bp)
AAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT13340.101607284038821No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT13160.10023627121071095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA207.8568904E-444.0000041
TCGTAAG351.4462967E-744.01
TATAGCG700.044.01
ACGTAAG254.4431865E-544.01
CCGAAAT254.4431865E-544.035
ATTGCGA254.4431865E-544.012
CGCAAGA502.7284841E-1144.032
CTACGGG1950.042.8717963
GACGGTC1950.042.87179629
CGTTTTT50300.042.2504961
CGACGGT2000.041.80000328
ACATACG3100.041.16129317
TACGAAA3050.041.11475420
CATACGA3050.041.11475418
CGGTCTA2050.040.7804931
ATACGAA3100.040.45161419
TTAACGG1200.040.3333322
CGCGAGG1100.040.02
TAATGCG557.8216544E-1140.01
TGCGTAG1100.040.01
Produced by FastQC (version 0.11.5)