##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547252_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1312898
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362343456993614	31.0	31.0	33.0	30.0	34.0
2	31.771418647907147	31.0	31.0	34.0	30.0	34.0
3	31.905666700688098	31.0	31.0	34.0	30.0	34.0
4	35.576303718948466	37.0	35.0	37.0	33.0	37.0
5	35.429945052852545	37.0	35.0	37.0	33.0	37.0
6	35.49426383466194	37.0	35.0	37.0	33.0	37.0
7	35.95297425999583	37.0	35.0	37.0	35.0	37.0
8	35.94698521895837	37.0	35.0	37.0	35.0	37.0
9	37.615816308654594	39.0	37.0	39.0	35.0	39.0
10	37.093698825042004	39.0	37.0	39.0	33.0	39.0
11	36.82479903236961	39.0	37.0	39.0	32.0	39.0
12	36.2461714466775	38.0	35.0	39.0	32.0	39.0
13	36.07532725314533	38.0	35.0	39.0	32.0	39.0
14	37.06692370618281	39.0	35.0	41.0	32.0	41.0
15	37.249699519688505	39.0	35.0	41.0	32.0	41.0
16	37.34008430205545	39.0	35.0	41.0	32.0	41.0
17	37.2528132421559	39.0	35.0	41.0	32.0	41.0
18	37.183038590964415	39.0	35.0	41.0	32.0	41.0
19	37.15402110445747	38.0	35.0	41.0	32.0	41.0
20	36.98660520466937	38.0	35.0	41.0	32.0	41.0
21	36.847611162481776	38.0	35.0	41.0	32.0	41.0
22	36.79906588325978	38.0	35.0	41.0	31.0	41.0
23	36.70630163196227	38.0	35.0	40.0	31.0	41.0
24	36.60267134232819	38.0	35.0	40.0	31.0	41.0
25	36.41396056662437	38.0	35.0	40.0	31.0	41.0
26	36.31461240705676	38.0	35.0	40.0	31.0	41.0
27	36.123270810070544	38.0	35.0	40.0	30.0	41.0
28	36.08264846164744	38.0	35.0	40.0	30.0	41.0
29	36.004455791691356	38.0	35.0	40.0	30.0	41.0
30	35.971891952002366	38.0	35.0	40.0	30.0	41.0
31	35.86535206847752	38.0	35.0	40.0	30.0	41.0
32	35.69295406040683	38.0	34.0	40.0	29.0	41.0
33	35.60579648990249	38.0	35.0	40.0	29.0	41.0
34	35.54698003957657	38.0	35.0	40.0	29.0	41.0
35	35.43846589757925	38.0	35.0	40.0	28.0	41.0
36	35.323102784831725	38.0	35.0	40.0	27.0	41.0
37	35.29638936154979	38.0	34.0	40.0	27.0	41.0
38	35.242293003721535	38.0	34.0	40.0	27.0	41.0
39	35.19140405423727	38.0	34.0	40.0	27.0	41.0
40	35.11009537679241	37.0	34.0	40.0	26.0	41.0
41	35.11695348762813	37.0	34.0	40.0	26.0	41.0
42	34.932112014794754	37.0	34.0	40.0	25.0	41.0
43	34.89402527842986	37.0	34.0	40.0	26.0	41.0
44	34.64723611430591	37.0	34.0	40.0	24.0	41.0
45	34.49925889139903	37.0	34.0	40.0	24.0	41.0
46	34.525904525713344	37.0	34.0	40.0	24.0	41.0
47	34.40909727945355	36.0	33.0	40.0	24.0	41.0
48	34.40756098341227	36.0	34.0	40.0	24.0	41.0
49	34.37245239157954	36.0	34.0	40.0	24.0	41.0
50	33.76784411279475	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	3.0
14	9.0
15	29.0
16	66.0
17	230.0
18	489.0
19	992.0
20	1798.0
21	2872.0
22	4489.0
23	6685.0
24	9991.0
25	14503.0
26	19868.0
27	23397.0
28	25684.0
29	28482.0
30	34018.0
31	40893.0
32	50576.0
33	64225.0
34	104424.0
35	177096.0
36	98868.0
37	118791.0
38	175122.0
39	309294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.29847253937473	26.130514327845727	28.576858217470054	12.994154915309492
2	30.68121057386027	27.267388631866297	28.977879469692237	13.073521324581193
3	28.129603365988824	25.550652068934525	31.982225580357348	14.3375189847193
4	24.010928495587624	29.283386828222756	31.2410408120052	15.46464386418442
5	21.895074864917152	32.8323297011649	30.60131099293319	14.671284440984753
6	19.601446570868415	41.305645983160915	27.49231090305568	11.600596542914987
7	84.3309228896685	4.328516000481378	8.745919332651889	2.5946417771982286
8	82.85053370482703	3.8479760042288125	7.9384689442744225	5.363021346669734
9	76.81480206383131	6.223712733205473	12.24900944323169	4.712475759731525
10	41.97020636789758	28.54608659621692	15.852488159780881	13.631218876104617
11	36.10090045075855	23.270657735787548	25.716849290653197	14.911592522800705
12	32.82638864557643	21.44172662308877	28.026701236501232	17.70518349483357
13	21.715929188710774	33.93660436682819	29.5360340254917	14.811432418969334
14	17.185950469876563	37.4360384431997	27.733456826044367	17.644554260879367
15	15.228067983956104	24.72362666406682	43.417843579623096	16.630461772353982
16	16.161118380864316	22.167449413435012	41.25552784755556	20.415904358145113
17	16.60761155855215	24.106747058796646	28.64685603908301	30.6387853435682
18	20.150384873767802	24.19395870814031	34.16335465512172	21.49230176297016
19	24.539758610341398	29.014058974878477	25.94146689232522	20.504715522454905
20	25.980083753650323	25.477607552147997	25.826530316901998	22.715778377299685
21	20.961719798491583	30.830117800468887	27.7180710154178	20.49009138562173
22	21.183595374507387	26.001410619865368	25.7958348630282	27.01915914259904
23	18.56427536640318	31.797519685459193	24.167223957992167	25.470980990145463
24	20.094097180435952	25.893405275962035	34.17272324278047	19.839774300821542
25	18.383606342610012	27.339519140100755	31.078194954977462	23.19867956231177
26	17.16987915283594	31.882598648181354	28.09174817845712	22.855774020525583
27	20.12456413217173	29.431380046279298	27.35102041438101	23.09303540716796
28	16.86041109057977	28.519351846068773	33.62157608588024	20.99866097747121
29	17.389088870574867	23.65248480841619	33.12961098272676	25.82881533828218
30	19.6257439648777	28.005755207182887	30.018173536710393	22.35032729122902
31	24.379426276831865	26.001715289382727	24.956775012224863	24.66208342156055
32	24.63694818637853	25.792178828819907	25.696664935128243	23.874208049673317
33	22.564662296690223	25.556059952867628	28.15953714606923	23.719740604372923
34	17.177419723390543	24.94481673366857	29.299381977884035	28.578381565056844
35	17.698099928554996	23.349033969127838	31.9771223659416	26.975743736375563
36	24.49413435011707	22.305540872177428	30.003016228221842	23.19730854948366
37	18.267527256496695	26.129447984534977	31.016042373436477	24.586982385531854
38	18.60654826193657	28.01778965311852	29.43122771152062	23.944434373424286
39	19.27019463812116	26.093649316245436	28.265790640247758	26.37036540538564
40	22.117102775691638	23.165470584919774	29.35178513486958	25.36564150451901
41	16.722395799216695	22.551409172685158	28.528872768486202	32.197322259611944
42	21.450790541230162	25.27233646482819	26.173320395034494	27.10355259890715
43	21.266617817987385	23.91610010831001	29.034319497782768	25.78296257591984
44	20.867500750248684	27.381030361840754	27.501527155955756	24.249941731954806
45	18.35946128335941	29.06014023937884	26.737796843319128	25.842601633942618
46	22.853641333904083	27.43922223965609	27.203408033221166	22.503728393218665
47	20.5242143715658	24.541358125307525	28.42193376789362	26.51249373523305
48	21.039562860176495	22.445917352300025	30.546318145050112	25.96820164247337
49	21.219851047072964	20.65499376189163	31.092362087534596	27.032793103500808
50	19.069798263079083	25.376685774523228	30.57404307112967	24.97947289126802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1266.0
1	1199.5
2	1133.0
3	5349.5
4	9566.0
5	6876.5
6	4187.0
7	4607.0
8	5027.0
9	5841.0
10	6655.0
11	7079.5
12	7504.0
13	7502.5
14	7501.0
15	7262.5
16	7024.0
17	6763.0
18	6502.0
19	6356.0
20	6210.0
21	6060.5
22	5911.0
23	5657.0
24	5403.0
25	5706.5
26	6010.0
27	6714.0
28	7418.0
29	8590.5
30	9763.0
31	11952.0
32	14141.0
33	15778.5
34	17416.0
35	22815.5
36	28215.0
37	31606.0
38	34997.0
39	43971.0
40	52945.0
41	70430.5
42	87916.0
43	99770.0
44	111624.0
45	131604.5
46	151585.0
47	161092.0
48	170599.0
49	165310.0
50	160021.0
51	132797.0
52	105573.0
53	88071.0
54	70569.0
55	61269.5
56	51970.0
57	46989.5
58	42009.0
59	38552.5
60	35096.0
61	31278.5
62	27461.0
63	23216.5
64	18972.0
65	15777.5
66	12583.0
67	9944.5
68	7306.0
69	6874.0
70	6442.0
71	4977.5
72	3513.0
73	2773.5
74	2034.0
75	1649.0
76	1264.0
77	1070.5
78	877.0
79	615.5
80	354.0
81	238.5
82	123.0
83	152.5
84	182.0
85	99.5
86	17.0
87	10.5
88	4.0
89	3.5
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1312898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.431577839717754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16057728598521	23.785496916757346
2	8.683356035022479	5.284964501795391
3	3.1516100402327387	2.8772539877933574
4	1.6007090961470767	1.9484841383257605
5	1.0192100691228545	1.5508085276768133
6	0.7704969420482304	1.4068462600323133
7	0.5794098828672626	1.2342649866103794
8	0.46055678031019154	1.1212375607695506
9	0.39377805284883766	1.0784958720157705
>10	3.949737060403966	26.971196236189826
>50	1.0547669936563289	22.351053984135998
>100	0.16950450237574666	7.377195779122564
>500	0.002514903591672666	0.5192729067175923
>1k	0.0035208650283417324	1.987242490173766
>5k	2.514903591672666E-4	0.5061858518835896
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6614	0.503771046951096	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	3482	0.265214814859951	TruSeq Adapter, Index 10 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3406	0.2594260940301531	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	3380	0.2574457421673275	RNA PCR Primer, Index 35 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2298	0.17503263772204694	RNA PCR Primer, Index 10 (95% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1768	0.13466392667214055	No Hit
AAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1692	0.12887520584234266	TruSeq Adapter, Index 10 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1474	0.11227071714634343	RNA PCR Primer, Index 35 (95% over 22bp)
AAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	1334	0.101607284038821	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	1316	0.10023627121071095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2804482907278402	0.0
2	0.0	0.0	0.0	0.9613084946431482	0.0
3	0.0	0.0	0.0	1.4335462465477136	0.0
4	0.0	0.0	0.0	2.239854124235089	0.0
5	0.0	0.0	0.0	3.5690510610877615	0.0
6	0.0	0.0	0.0	5.403085388202283	0.0
7	0.0	0.0	0.0	6.307649185237543	0.0
8	0.0	0.0	0.0	9.01235282558127	0.0
9	0.0	0.0	0.0	10.207647509555198	0.0
10	0.0	0.0	0.0	12.080451032753496	0.0
11	0.0	0.0	0.0	13.420996909127746	0.0
12	0.0	0.0	0.0	14.596792743990775	0.0
13	0.0	0.0	0.0	15.186404427457425	0.0
14	7.616737933944602E-5	0.0	0.0	15.449105718799176	0.0
15	1.5233475867889204E-4	0.0	0.0	15.75217572119083	0.0
16	1.5233475867889204E-4	0.0	0.0	16.37118801308251	0.0
17	2.2850213801833805E-4	0.0	0.0	17.023333114986848	0.0
18	2.2850213801833805E-4	0.0	0.0	17.947167258994988	0.0
19	2.2850213801833805E-4	0.0	0.0	18.33714424121295	0.0
20	2.2850213801833805E-4	0.0	0.0	18.839544275335935	0.0
21	2.2850213801833805E-4	0.0	0.0	19.341334970424207	0.0
22	2.2850213801833805E-4	0.0	0.0	19.855845617862165	0.0
23	2.2850213801833805E-4	0.0	0.0	20.435784044152708	0.0
24	2.2850213801833805E-4	0.0	0.0	20.869938106387547	0.0
25	2.2850213801833805E-4	0.0	0.0	21.2257159352821	0.0
26	2.2850213801833805E-4	0.0	0.0	21.570373326793096	0.0
27	2.2850213801833805E-4	0.0	0.0	21.94123229679686	0.0
28	2.2850213801833805E-4	0.0	0.0	22.294801271690567	0.0
29	2.2850213801833805E-4	0.0	0.0	22.65431130217275	0.0
30	2.2850213801833805E-4	0.0	0.0	23.117028131659886	0.0
31	2.2850213801833805E-4	0.0	0.0	23.49032445780251	0.0
32	2.2850213801833805E-4	0.0	0.0	23.844198102213575	0.0
33	2.2850213801833805E-4	0.0	0.0	24.203327295799063	0.0
34	2.2850213801833805E-4	0.0	0.0	24.575633446010276	0.0
35	2.2850213801833805E-4	0.0	0.0	24.992497513135064	0.0
36	2.2850213801833805E-4	0.0	0.0	25.369069036589284	0.0
37	2.2850213801833805E-4	0.0	0.0	25.73459629003929	0.0
38	2.2850213801833805E-4	0.0	0.0	26.10263706700749	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCGA	20	7.8568904E-4	44.000004	1
TCGTAAG	35	1.4462967E-7	44.0	1
TATAGCG	70	0.0	44.0	1
ACGTAAG	25	4.4431865E-5	44.0	1
CCGAAAT	25	4.4431865E-5	44.0	35
ATTGCGA	25	4.4431865E-5	44.0	12
CGCAAGA	50	2.7284841E-11	44.0	32
CTACGGG	195	0.0	42.871796	3
GACGGTC	195	0.0	42.871796	29
CGTTTTT	5030	0.0	42.250496	1
CGACGGT	200	0.0	41.800003	28
ACATACG	310	0.0	41.161293	17
TACGAAA	305	0.0	41.114754	20
CATACGA	305	0.0	41.114754	18
CGGTCTA	205	0.0	40.78049	31
ATACGAA	310	0.0	40.451614	19
TTAACGG	120	0.0	40.333332	2
CGCGAGG	110	0.0	40.0	2
TAATGCG	55	7.8216544E-11	40.0	1
TGCGTAG	110	0.0	40.0	1
>>END_MODULE