FastQCFastQC Report
Thu 26 May 2016
SRR1547251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547251_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1618169
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63770.3940873913664148No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC29030.1794002974967386TruSeq Adapter, Index 19 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26590.16432152636714706No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG25470.15740012322569522No Hit
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG20150.12452345830379892No Hit
GAGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC18650.1152537219536402No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT17280.10678736275382857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.857434E-444.00000418
CCTTACG207.857434E-444.0000041
GGCGTAA207.857434E-444.0000048
TCGACTT207.857434E-444.00000411
GATCGGA254.4436485E-544.010
CGCGACA254.4436485E-544.017
GCGATCG302.5283607E-644.09
TCGGTAC502.7284841E-1144.036
TACGTAG454.802132E-1044.01
GTCGGAA254.4436485E-544.017
CGACGGT5100.042.27450628
CGGTCTA5100.042.27450631
CGTTTTT61000.041.9081951
CGCATCG1800.041.55555721
GCTACGA1800.041.55555710
CACGACG5350.041.12149426
TCACGAC5450.040.7706425
TTCGGTA557.8216544E-1140.00000435
CTAAGCG501.3478711E-939.61
TCGATTG501.3478711E-939.61
Produced by FastQC (version 0.11.5)