##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547251_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1618169
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404706183346732	31.0	31.0	33.0	30.0	34.0
2	31.823860795751248	31.0	31.0	34.0	30.0	34.0
3	31.951143545575277	33.0	31.0	34.0	30.0	34.0
4	35.62644692859646	37.0	35.0	37.0	33.0	37.0
5	35.496996296431334	37.0	35.0	37.0	33.0	37.0
6	35.560463091308755	37.0	35.0	37.0	33.0	37.0
7	35.97846578447616	37.0	35.0	37.0	35.0	37.0
8	35.97116803003889	37.0	35.0	37.0	35.0	37.0
9	37.652199492142046	39.0	37.0	39.0	35.0	39.0
10	37.11271257822885	39.0	37.0	39.0	33.0	39.0
11	36.8918870649481	39.0	37.0	39.0	33.0	39.0
12	36.38228083716843	38.0	35.0	39.0	32.0	39.0
13	36.25259722562971	38.0	35.0	39.0	32.0	39.0
14	37.260282455046415	39.0	35.0	41.0	32.0	41.0
15	37.419893719382834	39.0	35.0	41.0	32.0	41.0
16	37.48949213586467	39.0	35.0	41.0	33.0	41.0
17	37.386293397043204	39.0	35.0	41.0	32.0	41.0
18	37.328811143953445	39.0	35.0	41.0	32.0	41.0
19	37.30961908181408	39.0	35.0	41.0	32.0	41.0
20	37.135714502008135	39.0	35.0	41.0	32.0	41.0
21	37.001650631052755	38.0	35.0	41.0	32.0	41.0
22	36.95225097007791	38.0	35.0	41.0	32.0	41.0
23	36.88203518915515	38.0	35.0	41.0	32.0	41.0
24	36.80062465663352	38.0	35.0	41.0	32.0	41.0
25	36.63960562833672	38.0	35.0	40.0	31.0	41.0
26	36.546544273187784	38.0	35.0	40.0	31.0	41.0
27	36.39502857859717	38.0	35.0	40.0	31.0	41.0
28	36.36236820752344	38.0	35.0	40.0	31.0	41.0
29	36.32976716276236	38.0	35.0	40.0	31.0	41.0
30	36.290598818788396	38.0	35.0	40.0	31.0	41.0
31	36.225938081869074	38.0	35.0	40.0	30.0	41.0
32	36.08154587067234	38.0	35.0	40.0	30.0	41.0
33	35.99766958828157	38.0	35.0	40.0	30.0	41.0
34	35.910353615722464	38.0	35.0	41.0	30.0	41.0
35	35.757261447969896	38.0	35.0	41.0	29.0	41.0
36	35.62347628708744	38.0	35.0	40.0	29.0	41.0
37	35.57180492272439	38.0	35.0	40.0	28.0	41.0
38	35.492658677801884	38.0	35.0	40.0	28.0	41.0
39	35.434740129121245	38.0	35.0	40.0	27.0	41.0
40	35.35502163247473	38.0	35.0	40.0	27.0	41.0
41	35.36445760609677	38.0	35.0	40.0	27.0	41.0
42	35.20794428764857	38.0	34.0	40.0	27.0	41.0
43	35.18152862896274	37.0	34.0	40.0	27.0	41.0
44	34.96174565203017	37.0	34.0	40.0	26.0	41.0
45	34.80219803988335	37.0	34.0	40.0	25.0	41.0
46	34.83815720113289	37.0	34.0	40.0	26.0	41.0
47	34.72712491711311	37.0	34.0	40.0	26.0	41.0
48	34.7012394873465	37.0	34.0	40.0	26.0	41.0
49	34.64229755977281	36.0	34.0	40.0	26.0	41.0
50	34.04963573026056	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	3.0
14	17.0
15	26.0
16	64.0
17	206.0
18	495.0
19	1028.0
20	1833.0
21	3194.0
22	4911.0
23	7327.0
24	10869.0
25	16758.0
26	22278.0
27	26829.0
28	28807.0
29	32168.0
30	37982.0
31	46544.0
32	59193.0
33	75870.0
34	123925.0
35	215254.0
36	121718.0
37	151604.0
38	222065.0
39	407196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.38452287740032	26.011621777453403	28.22393705478229	12.379918290363985
2	32.18600776556713	25.939132439195163	28.346915557027724	13.527944238209976
3	29.18100643381501	25.86967121481131	31.28455680463536	13.66476554673832
4	24.873730741350254	29.208074063957472	30.762485253394424	15.155709941297848
5	22.472621833689807	31.70120055445383	30.588152411769105	15.238025200087261
6	19.67118391218717	41.59818906430664	27.11984965723605	11.610777366270149
7	85.40974397606183	3.7648107212534665	8.273610481970673	2.5518348207140296
8	84.0023508051384	3.298728377567485	7.610391745237982	5.088529072056133
9	77.58472693519651	5.120107973889007	12.451109865533203	4.84405522538128
10	41.201815137973846	23.22989749525544	19.049864383757196	16.51842298301352
11	35.0478843680728	24.796791929643938	24.49373334923608	15.661590353047178
12	31.730678316047335	20.550387505878557	27.918344746438724	19.800589431635384
13	21.76515555544569	32.47652130278111	29.658274259363516	16.100048882409688
14	17.176574263874787	36.21469698158845	28.297785954371886	18.310942800164877
15	15.865153763296663	24.7985222804293	42.81851895568386	16.517805000590176
16	16.63441828387517	21.21125790940254	41.83314598166199	20.3211778250603
17	16.63571604696419	23.618608439538765	29.634914523761115	30.11076098973593
18	20.397127864889267	24.105640387376102	33.4883439245221	22.008887823212532
19	24.75983658072797	29.36090111725042	25.884317398244562	19.994944903777046
20	26.610137754461988	24.46926124527166	25.41638110728855	23.504219892977805
21	21.738520512999568	30.92550901667255	26.962882121706695	20.373088348621188
22	21.234432250277937	26.22303356447936	25.092867308668005	27.449666876574696
23	18.81867715918424	31.79636984764879	24.2903553337136	25.09459765945337
24	19.277405512032427	25.649978463312546	34.17912467733593	20.8934913473191
25	19.209242050737593	26.34730982981382	31.57482314888	22.86862497056859
26	17.307524739381364	31.03489190560442	29.360097740100077	22.29748561491414
27	20.86951362929336	29.69010035416573	26.642211042233537	22.79817497430738
28	18.715350498001136	28.37021349438779	33.085604779228866	19.828831228382203
29	19.174017052606988	24.72331381950835	31.72400410587522	24.378665022009443
30	21.60342955525659	27.042910845529732	29.712224124921438	21.64143547429224
31	26.00698690927833	26.06544804652666	23.8116661485914	24.11589889560361
32	25.583607151045413	25.710726135527253	27.02350619743673	21.682160515990603
33	25.856075601497743	26.15270716470282	25.261576510240896	22.729640723558543
34	20.683748112836177	25.490477199847483	28.399135071800284	25.426639615516056
35	20.25573348642818	25.6471975424075	28.997218461112528	25.09985051005179
36	27.335834514194744	24.233871740219964	26.570895870579648	21.85939787500564
37	21.469945351814303	28.158801707361842	27.9384291751974	22.43282376562646
38	21.568019162398983	28.5461530903138	27.502566172012937	22.383261575274275
39	20.531415445481898	26.948359534758115	28.3353592857112	24.184865734048792
40	22.916580406620074	23.06736811791599	30.235531641009068	23.780519834454868
41	17.475863151500246	22.219310838361135	30.68585543289978	29.61897057723884
42	21.87472383910457	25.17271063776404	27.777382955673975	25.175182567457416
43	21.073138837785176	25.344756944422986	29.190894152588516	24.391210065203325
44	21.863661953726712	27.97872162919942	27.438666789439175	22.71894962763469
45	19.650975886943822	28.57760839566201	26.64777288404363	25.123642833350534
46	23.392241477867888	26.8230944975463	27.062562686592067	22.722101337993745
47	22.042815058254114	24.51907062859318	28.681862030480126	24.756252282672577
48	22.191192638098986	22.451610431296114	29.73490407985816	25.622292850746742
49	22.235007591914073	21.632289334426748	29.891439027691174	26.241264045968006
50	20.315863176219544	25.22826725762266	30.052732440183938	24.403137125973863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1293.0
1	1350.0
2	1407.0
3	5942.5
4	10478.0
5	7817.5
6	5157.0
7	5853.0
8	6549.0
9	7337.0
10	8125.0
11	8430.0
12	8735.0
13	8821.5
14	8908.0
15	8720.0
16	8532.0
17	8275.0
18	8018.0
19	7715.0
20	7412.0
21	7514.5
22	7617.0
23	7688.0
24	7759.0
25	7919.5
26	8080.0
27	9583.0
28	11086.0
29	13028.0
30	14970.0
31	16800.5
32	18631.0
33	21973.0
34	25315.0
35	30612.0
36	35909.0
37	39442.5
38	42976.0
39	50746.5
40	58517.0
41	76643.5
42	94770.0
43	112654.0
44	130538.0
45	147664.0
46	164790.0
47	182075.5
48	199361.0
49	195812.0
50	192263.0
51	166347.5
52	140432.0
53	118953.5
54	97475.0
55	84424.0
56	71373.0
57	63828.0
58	56283.0
59	51702.5
60	47122.0
61	42461.0
62	37800.0
63	31421.0
64	25042.0
65	21926.0
66	18810.0
67	15414.0
68	12018.0
69	10718.0
70	9418.0
71	7612.0
72	5806.0
73	5012.0
74	4218.0
75	3224.5
76	2231.0
77	1799.0
78	1367.0
79	1081.5
80	796.0
81	667.0
82	538.0
83	352.5
84	167.0
85	108.5
86	50.0
87	31.5
88	13.0
89	9.5
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1618169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.150915530680255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50877043847301	22.59451619938813
2	8.876195209649136	5.174984335806214
3	3.213695822386252	2.8104652637904493
4	1.6459955151764714	1.9192910490715138
5	1.0670362002722769	1.5552541071157582
6	0.7893812135534176	1.3806711046680928
7	0.6027510723290833	1.2299521916844434
8	0.5110241176285655	1.1917456709704577
9	0.4230929771023904	1.1100192873422237
>10	4.020789543993261	25.716374606005388
>50	1.068515717861366	22.35483107100737
>100	0.2671988782871865	10.53582056205521
>500	0.0019222938303406452	0.3793056888302236
>1k	0.0034174112539389254	1.64971824216113
>5k	2.1358820337118284E-4	0.3970506201034695
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6377	0.3940873913664148	No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2903	0.1794002974967386	TruSeq Adapter, Index 19 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2659	0.16432152636714706	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	2547	0.15740012322569522	No Hit
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	2015	0.12452345830379892	No Hit
GAGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	1865	0.1152537219536402	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT	1728	0.10678736275382857	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18768126196954707	0.0
2	0.0	0.0	0.0	0.6504882988118051	0.0
3	0.0	0.0	0.0	1.0448228831475574	0.0
4	0.0	0.0	0.0	1.653226578929642	0.0
5	0.0	0.0	0.0	2.657509815105839	0.0
6	0.0	0.0	0.0	4.438040773244327	0.0
7	0.0	0.0	0.0	5.339738927145434	0.0
8	0.0	0.0	0.0	8.163609610615454	0.0
9	0.0	0.0	0.0	9.522305766579388	0.0
10	0.0	0.0	0.0	11.544220659276009	0.0
11	0.0	0.0	0.0	12.872079492315079	0.0
12	0.0	0.0	0.0	14.02165039621943	0.0
13	0.0	0.0	0.0	14.614295540206246	0.0
14	0.0	0.0	0.0	14.872797587891005	0.0
15	0.0	0.0	0.0	15.13024906545608	0.0
16	0.0	0.0	0.0	15.678893860900809	0.0
17	0.0	0.0	0.0	16.25602764606169	0.0
18	0.0	0.0	0.0	17.109832162153644	0.0
19	0.0	0.0	0.0	17.456149512195573	0.0
20	0.0	0.0	0.0	17.865192078206913	0.0
21	0.0	0.0	0.0	18.341718324847406	0.0
22	0.0	0.0	0.0	18.80829505447206	0.0
23	0.0	0.0	0.0	19.382215331031556	0.0
24	0.0	0.0	0.0	19.791319695285228	0.0
25	0.0	0.0	0.0	20.13417634375643	0.0
26	0.0	0.0	0.0	20.46078005449369	0.0
27	0.0	0.0	0.0	20.817912096944138	0.0
28	0.0	0.0	0.0	21.15230238621553	0.0
29	0.0	0.0	0.0	21.50708609545727	0.0
30	0.0	0.0	0.0	21.96223015025007	0.0
31	0.0	0.0	0.0	22.350570305079383	0.0
32	0.0	0.0	0.0	22.696084277970964	0.0
33	0.0	0.0	0.0	23.05117697842438	0.0
34	0.0	0.0	0.0	23.386308846603786	0.0
35	0.0	0.0	0.0	23.7744636067061	0.0
36	0.0	0.0	0.0	24.128320342312826	0.0
37	0.0	0.0	0.0	24.498059226199487	0.0
38	0.0	0.0	0.0	24.865449776877448	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.857434E-4	44.000004	18
CCTTACG	20	7.857434E-4	44.000004	1
GGCGTAA	20	7.857434E-4	44.000004	8
TCGACTT	20	7.857434E-4	44.000004	11
GATCGGA	25	4.4436485E-5	44.0	10
CGCGACA	25	4.4436485E-5	44.0	17
GCGATCG	30	2.5283607E-6	44.0	9
TCGGTAC	50	2.7284841E-11	44.0	36
TACGTAG	45	4.802132E-10	44.0	1
GTCGGAA	25	4.4436485E-5	44.0	17
CGACGGT	510	0.0	42.274506	28
CGGTCTA	510	0.0	42.274506	31
CGTTTTT	6100	0.0	41.908195	1
CGCATCG	180	0.0	41.555557	21
GCTACGA	180	0.0	41.555557	10
CACGACG	535	0.0	41.121494	26
TCACGAC	545	0.0	40.77064	25
TTCGGTA	55	7.8216544E-11	40.000004	35
CTAAGCG	50	1.3478711E-9	39.6	1
TCGATTG	50	1.3478711E-9	39.6	1
>>END_MODULE