FastQCFastQC Report
Thu 26 May 2016
SRR1547250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547250_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1367565
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79830.5837382501014577No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC29470.21549249944243964No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC13850.10127489369792296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAT207.857007E-444.032
TACTACG351.4463512E-744.01
CACGACG1500.042.53333326
CGTTTTT52000.041.5884631
CTCACGA1600.041.2524
TCACGAC1650.040.025
TATAGCG501.3478711E-939.61
CATATGC12350.039.1902833
GCCAGTA12800.038.8437525
CGGTCTA1600.038.531
TAACGGG3450.038.2608683
AGGGTAC8850.038.03396
TAGCATA12650.037.73913230
TACGGGT1750.037.7142874
ATACGAG357.2912226E-637.7142871
CCAGTAG13350.037.57303226
CGTATGG1650.037.3333322
GGTACCT14300.037.230778
TGCCAGT13450.037.13011624
ACGGGAT3200.037.1255
Produced by FastQC (version 0.11.5)