##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547250_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1367565
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.371012712375645	31.0	31.0	33.0	30.0	34.0
2	31.812493007644974	31.0	31.0	34.0	30.0	34.0
3	31.986610508458465	33.0	31.0	34.0	30.0	34.0
4	35.677373287558545	37.0	35.0	37.0	33.0	37.0
5	35.55541126015948	37.0	35.0	37.0	33.0	37.0
6	35.61261073513873	37.0	35.0	37.0	33.0	37.0
7	35.91921700248251	37.0	35.0	37.0	35.0	37.0
8	35.901355328631546	37.0	35.0	37.0	35.0	37.0
9	37.58939136348181	39.0	37.0	39.0	35.0	39.0
10	37.14414159473224	39.0	37.0	39.0	33.0	39.0
11	36.9107691407721	39.0	37.0	39.0	33.0	39.0
12	36.51227619893753	39.0	35.0	39.0	32.0	39.0
13	36.42437178488774	39.0	35.0	39.0	32.0	39.0
14	37.43140472299306	40.0	35.0	41.0	32.0	41.0
15	37.54961190144527	40.0	35.0	41.0	32.0	41.0
16	37.582341607163094	40.0	35.0	41.0	33.0	41.0
17	37.46665716071997	39.0	35.0	41.0	32.0	41.0
18	37.41961734908396	39.0	36.0	41.0	32.0	41.0
19	37.40481220271066	39.0	35.0	41.0	32.0	41.0
20	37.237357639307824	39.0	35.0	41.0	32.0	41.0
21	37.13040038316277	39.0	35.0	41.0	32.0	41.0
22	37.10540851805947	39.0	35.0	41.0	32.0	41.0
23	37.02571870441259	39.0	35.0	41.0	32.0	41.0
24	36.931440918713186	39.0	35.0	41.0	31.0	41.0
25	36.71448450347881	38.0	35.0	41.0	31.0	41.0
26	36.63100620445829	38.0	35.0	41.0	31.0	41.0
27	36.47295448479597	38.0	35.0	40.0	31.0	41.0
28	36.40567212527375	38.0	35.0	40.0	30.0	41.0
29	36.34178777608377	38.0	35.0	40.0	30.0	41.0
30	36.27569731603251	38.0	35.0	40.0	30.0	41.0
31	36.19239304895928	38.0	35.0	40.0	30.0	41.0
32	36.090890743767204	38.0	35.0	40.0	30.0	41.0
33	35.995214852676106	38.0	35.0	40.0	30.0	41.0
34	35.85346656283248	38.0	35.0	41.0	29.0	41.0
35	35.68388339859531	38.0	35.0	40.0	28.0	41.0
36	35.54540807932347	38.0	35.0	40.0	27.0	41.0
37	35.496159231919506	38.0	34.0	40.0	27.0	41.0
38	35.358045138622295	38.0	34.0	40.0	27.0	41.0
39	35.2992676764907	38.0	34.0	40.0	27.0	41.0
40	35.16113237762008	37.0	34.0	40.0	26.0	41.0
41	35.122650842921544	37.0	34.0	40.0	26.0	41.0
42	34.9530099117775	37.0	34.0	40.0	26.0	41.0
43	34.938435833031704	37.0	34.0	40.0	26.0	41.0
44	34.70206388727409	37.0	34.0	40.0	26.0	41.0
45	34.54613930599277	36.0	34.0	40.0	24.0	41.0
46	34.59428034499274	36.0	34.0	40.0	25.0	41.0
47	34.47355847802481	36.0	33.0	40.0	24.0	41.0
48	34.41712825350166	36.0	33.0	40.0	24.0	41.0
49	34.32178872667844	36.0	33.0	40.0	24.0	41.0
50	33.74814871688	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	5.0
14	19.0
15	32.0
16	79.0
17	206.0
18	461.0
19	965.0
20	1671.0
21	2783.0
22	4555.0
23	6705.0
24	9471.0
25	13716.0
26	18680.0
27	23246.0
28	25949.0
29	28868.0
30	33648.0
31	40730.0
32	50166.0
33	64384.0
34	104529.0
35	146722.0
36	113263.0
37	148388.0
38	207791.0
39	320526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.23069470189717	24.24835382596074	27.361770738502372	14.159180733639717
2	33.17421841009385	25.15098002654353	28.430970374351496	13.243831189011127
3	30.255673404920426	25.030473871443036	30.6690358410752	14.044816882561339
4	26.583818685035084	27.726799091816478	30.301960053087058	15.387422170061388
5	23.29885599587588	32.087615579515415	29.016756059126987	15.596772365481716
6	21.40315085571801	40.89516768855594	26.467114908614946	11.234566547111106
7	85.98911203489413	3.5400876740776486	8.081736517094251	2.389063773933963
8	84.72708792635085	3.357061638752089	7.517741387063868	4.398109047833192
9	78.37397125547963	4.667858566137624	11.818743533214143	5.139426645168602
10	44.16119160698029	22.084580988837825	17.346451539780556	16.407775864401327
11	34.299576254145144	23.268656334433828	24.11351562814199	18.31825178327904
12	30.374717106682315	18.187947190809943	29.382369393776532	22.05496630873121
13	24.619378237963094	22.45458168350316	32.15876393443822	20.76727614409553
14	20.929096606011413	25.625034276250126	32.04176766735036	21.4041014503881
15	20.456870422978067	20.530066212574905	38.120674337234426	20.8923890272126
16	23.916961899434398	18.47722046118466	35.2043230120689	22.401494627312047
17	22.680238233648858	20.982329907536386	29.950825006489634	26.386606852325116
18	24.40110707717732	21.118118700025228	31.480112462661737	23.000661760135717
19	27.049975686713246	25.84308606903511	25.815518823602535	21.29141942064911
20	27.943095940595146	24.07234756666045	24.953110089831195	23.031446402913208
21	25.902315429248336	26.924131576926875	26.261420846541117	20.91213214728368
22	24.47145108276389	21.975116356443753	27.103720846906725	26.449711713885627
23	23.98767151835562	25.6041943161751	25.33780844054944	25.07032572491984
24	24.394745405154417	23.33958532135584	29.675664410832393	22.590004862657352
25	25.125533338451923	22.412243659350743	27.24572506608461	25.216497936112724
26	21.303411538025614	24.332298647596275	28.386292424857317	25.97799738952079
27	23.42989181501428	22.15989733577563	27.82434472950097	26.585866119709117
28	23.152830030016855	24.307583186173968	29.376665825756	23.16292095805318
29	24.6171845579552	22.940701173253192	27.887668959062275	24.554445309729335
30	25.73208586063551	23.003221053478264	28.759144903532924	22.50554818235331
31	29.064724528633008	21.852709012003086	24.02766961716628	25.05489684219763
32	28.676955025903705	22.5505186225152	25.328375616515487	23.44415073506561
33	28.626719753722856	22.852368991601864	23.842523024499748	24.67838823017553
34	23.71514333870785	23.581840716894625	26.85832117668996	25.844694767707566
35	23.831262133792546	26.167238851535394	26.12928818739877	23.872210827273292
36	26.974513094441583	26.058871059145268	22.450194323487366	24.516421522925786
37	25.359964608629205	28.38022324350214	23.345362012043303	22.914450135825355
38	24.65491585409103	28.855447455879613	22.56697122257443	23.92266546745493
39	25.034861231458834	26.42229071378691	22.46386826220326	26.078979792550992
40	25.362012043303245	23.421263340316546	26.342660129500246	24.874064486879966
41	23.222442808934126	22.852661482269582	26.46930858862284	27.455587120173448
42	24.40074146384267	24.38355763711414	25.01533747938855	26.200363419654643
43	23.36459327344587	22.491069894301184	27.71597693711085	26.428359895142094
44	24.52029702427307	23.678143269241318	26.675807000032904	25.125752706452708
45	22.95437511196908	23.862266144570825	27.649947168873148	25.53341157458695
46	25.049558887511747	23.870967741935484	26.378124622961245	24.701348747591524
47	23.824607971101923	23.615184653014666	27.12602326032035	25.434184115563063
48	23.742491216139637	22.842570554233255	27.751148939904134	25.663789289722978
49	25.199460354718056	21.184879694932235	27.173918607159443	26.441741343190266
50	22.959713066654967	22.256199888122318	28.664377927191758	26.119709118030954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	724.0
1	968.0
2	1212.0
3	7450.5
4	13689.0
5	9467.5
6	5246.0
7	5378.0
8	5510.0
9	5761.5
10	6013.0
11	5966.0
12	5919.0
13	5878.0
14	5837.0
15	5600.0
16	5363.0
17	5120.0
18	4877.0
19	4669.5
20	4462.0
21	4264.5
22	4067.0
23	4244.0
24	4421.0
25	4835.5
26	5250.0
27	6058.5
28	6867.0
29	7737.0
30	8607.0
31	10038.5
32	11470.0
33	13344.0
34	15218.0
35	17747.0
36	20276.0
37	22183.5
38	24091.0
39	28611.5
40	33132.0
41	40957.0
42	48782.0
43	56910.5
44	65039.0
45	78151.0
46	91263.0
47	108939.0
48	126615.0
49	130892.5
50	135170.0
51	122630.5
52	110091.0
53	98655.0
54	87219.0
55	83983.0
56	80747.0
57	82193.5
58	83640.0
59	81048.5
60	78457.0
61	74939.5
62	71422.0
63	66720.5
64	62019.0
65	52378.5
66	42738.0
67	36791.0
68	30844.0
69	26498.0
70	22152.0
71	19108.0
72	16064.0
73	13096.5
74	10129.0
75	8224.5
76	6320.0
77	4946.5
78	3573.0
79	2728.5
80	1884.0
81	1286.5
82	689.0
83	467.5
84	246.0
85	184.5
86	123.0
87	76.5
88	30.0
89	36.5
90	43.0
91	28.0
92	13.0
93	6.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1367565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.11005752760018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4503421628653	21.67253743310526
2	6.911167701896479	4.023689787701977
3	2.9487411064376525	2.5751406972719835
4	1.8451826852453297	2.1485349646569327
5	1.3428880562521197	1.9545774285313195
6	1.1046796782462736	1.9294373389991928
7	0.9592740016248587	1.9547164960421606
8	0.7795533776573353	1.815427493555205
9	0.7275006752059536	1.9059827855951956
>10	8.677900499791749	52.17336648627452
>50	0.20642774838810887	3.7538028015753926
>100	0.04102433695294158	2.1879304399481527
>500	0.004051786236995274	0.8310891956725395
>1k	0.0010129465592488185	0.4852811934639283
>5k	2.532366398122046E-4	0.5884854576063404
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7983	0.5837382501014577	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2947	0.21549249944243964	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1385	0.10127489369792296	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10690533905152588	0.0
2	0.0	0.0	0.0	0.32049664915378795	0.0
3	0.0	0.0	0.0	0.5365741299316669	0.0
4	0.0	0.0	0.0	0.8456636430443891	0.0
5	0.0	0.0	0.0	1.396716061028178	0.0
6	0.0	0.0	0.0	2.663785633589628	0.0
7	0.0	0.0	0.0	3.3590359507591963	0.0
8	0.0	0.0	0.0	5.510158566503238	0.0
9	0.0	0.0	0.0	6.741251786935173	0.0
10	0.0	0.0	0.0	8.40896045160559	0.0
11	0.0	0.0	0.0	9.265738740023327	0.0
12	0.0	0.0	0.0	10.013344886714709	0.0
13	0.0	0.0	0.0	10.444987989601957	0.0
14	0.0	0.0	0.0	10.694482529166804	0.0
15	0.0	0.0	0.0	10.867783249790687	0.0
16	0.0	0.0	0.0	11.150329234807852	0.0
17	0.0	0.0	0.0	11.416203251764998	0.0
18	0.0	0.0	0.0	11.832856207931616	0.0
19	0.0	0.0	0.0	12.038915883340097	0.0
20	0.0	0.0	0.0	12.272762172181944	0.0
21	0.0	0.0	0.0	12.508582773030898	0.0
22	0.0	0.0	0.0	12.757565453927235	0.0
23	0.0	0.0	0.0	13.039160844274312	0.0
24	0.0	0.0	0.0	13.27132531177677	0.0
25	0.0	0.0	0.0	13.461444245794533	0.0
26	0.0	0.0	0.0	13.649881358472907	0.0
27	0.0	0.0	0.0	13.872393633940616	0.0
28	0.0	0.0	0.0	14.056589631937056	0.0
29	0.0	0.0	0.0	14.257384475326583	0.0
30	0.0	0.0	0.0	14.565304025768429	0.0
31	0.0	0.0	0.0	14.78635384789754	0.0
32	0.0	0.0	0.0	15.010840435372359	0.0
33	0.0	0.0	0.0	15.236935721519636	0.0
34	0.0	0.0	0.0	15.466394650345688	0.0
35	0.0	0.0	0.0	15.733365507306782	0.0
36	0.0	0.0	0.0	15.969771089491175	0.0
37	0.0	0.0	0.0	16.203105519664515	0.0
38	0.0	0.0	0.0	16.496839272721953	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCAT	20	7.857007E-4	44.0	32
TACTACG	35	1.4463512E-7	44.0	1
CACGACG	150	0.0	42.533333	26
CGTTTTT	5200	0.0	41.588463	1
CTCACGA	160	0.0	41.25	24
TCACGAC	165	0.0	40.0	25
TATAGCG	50	1.3478711E-9	39.6	1
CATATGC	1235	0.0	39.19028	33
GCCAGTA	1280	0.0	38.84375	25
CGGTCTA	160	0.0	38.5	31
TAACGGG	345	0.0	38.260868	3
AGGGTAC	885	0.0	38.0339	6
TAGCATA	1265	0.0	37.739132	30
TACGGGT	175	0.0	37.714287	4
ATACGAG	35	7.2912226E-6	37.714287	1
CCAGTAG	1335	0.0	37.573032	26
CGTATGG	165	0.0	37.333332	2
GGTACCT	1430	0.0	37.23077	8
TGCCAGT	1345	0.0	37.130116	24
ACGGGAT	320	0.0	37.125	5
>>END_MODULE