FastQCFastQC Report
Thu 26 May 2016
SRR1547249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547249_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277997
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66680.5217539634287092No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC28800.22535264167286775No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCT27950.21870160884571715No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27860.21799738184048947No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG22220.17386582284621951No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCT19740.15446045647994477No Hit
GAGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC19650.1537562294747171No Hit
TAGAGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCT19160.1499221046684773No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG16350.12793457261636765No Hit
AAGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC16210.12683910838601342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15230.1191708587735339No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCT12810.10023497707741097No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA207.856812E-444.00000413
CACGCTA207.856812E-444.00000435
TCTAGCG408.314601E-944.0000041
CGAATTA207.856812E-444.00000436
TAATGCG408.314601E-944.0000041
ATCGACG207.856812E-444.0000041
TAAACGG1150.044.02
CTACGGC302.5279405E-644.03
GTCGTAA254.4431217E-544.032
CGTCAAA254.4431217E-544.035
TAACTAC254.4431217E-544.023
TAGTAAG1650.044.01
CGTTTTT57450.042.3916471
TCGATAG850.041.4117661
CGCATGG800.041.2500042
GCGTAAG800.041.2500041
AACGAAG603.6379788E-1240.3333321
ATTAGCG557.8216544E-1140.0000041
ATATACG557.8216544E-1140.0000041
TATACGG1050.039.8095252
Produced by FastQC (version 0.11.5)