FastQCFastQC Report
Thu 26 May 2016
SRR1547248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547248_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1454583
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70300.4833000248181094No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25660.17640794646988173No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG17370.11941566758308052No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC17360.11934691935764409No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT16730.11501578115514893No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT15020.10325983460551925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGCG207.857171E-444.0000041
TGCGCAA207.857171E-444.00000413
CTACGCG302.5281824E-644.0000041
TTACGAG408.314601E-944.0000041
TACTACG302.5281824E-644.0000041
TCGTTAG850.044.01
CGTGCCG254.443426E-544.02
TACGAAG502.7284841E-1144.01
CGTTTTT61900.041.58321
CGGTCTA1700.041.41176631
ACGGGAT6450.039.2248045
CGACGGT1800.039.1111128
GACCGAC452.3515895E-839.111119
CGAGGGA8550.038.85384
ATTACGG850.038.8235322
TATTACG404.1255407E-738.5000041
CCGCATC404.1255407E-738.50000420
CGACAGG1550.038.3225822
TAGGGAC12850.038.178995
GCTACGA2200.038.010
Produced by FastQC (version 0.11.5)