##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547248_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1454583
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.445506375366687	31.0	31.0	33.0	30.0	34.0
2	31.87297459134336	31.0	31.0	34.0	30.0	34.0
3	31.99548049165981	33.0	31.0	34.0	30.0	34.0
4	35.65079682630692	37.0	35.0	37.0	33.0	37.0
5	35.50719209560403	37.0	35.0	37.0	33.0	37.0
6	35.570096034396116	37.0	35.0	37.0	33.0	37.0
7	36.00690919665636	37.0	35.0	37.0	35.0	37.0
8	36.00874752420453	37.0	35.0	37.0	35.0	37.0
9	37.66813719120875	39.0	37.0	39.0	35.0	39.0
10	37.17382645060474	39.0	37.0	39.0	33.0	39.0
11	36.89765932916857	39.0	37.0	39.0	33.0	39.0
12	36.321103711510446	38.0	35.0	39.0	32.0	39.0
13	36.15601859776994	38.0	35.0	39.0	32.0	39.0
14	37.16760473620275	39.0	35.0	41.0	32.0	41.0
15	37.36107117985017	39.0	35.0	41.0	32.0	41.0
16	37.44618217042272	39.0	35.0	41.0	33.0	41.0
17	37.36111449123219	39.0	35.0	41.0	32.0	41.0
18	37.29124841965017	39.0	35.0	41.0	32.0	41.0
19	37.27171567383917	39.0	35.0	41.0	32.0	41.0
20	37.09980592375959	39.0	35.0	41.0	32.0	41.0
21	36.95760365685561	38.0	35.0	41.0	32.0	41.0
22	36.908239681063236	38.0	35.0	41.0	32.0	41.0
23	36.847909675831495	38.0	35.0	41.0	32.0	41.0
24	36.77467081630955	38.0	35.0	41.0	32.0	41.0
25	36.60183296518659	38.0	35.0	41.0	31.0	41.0
26	36.50074763695162	38.0	35.0	40.0	31.0	41.0
27	36.34594863270092	38.0	35.0	40.0	31.0	41.0
28	36.324783116535805	38.0	35.0	40.0	31.0	41.0
29	36.30270668638366	38.0	35.0	40.0	31.0	41.0
30	36.29739519848644	38.0	35.0	40.0	31.0	41.0
31	36.17999522887315	38.0	35.0	40.0	30.0	41.0
32	36.024456493716755	38.0	35.0	40.0	30.0	41.0
33	35.847653244950614	38.0	35.0	40.0	30.0	41.0
34	35.79332083490595	38.0	35.0	40.0	30.0	41.0
35	35.608765536239595	38.0	35.0	40.0	29.0	41.0
36	35.468728150954604	38.0	35.0	40.0	27.0	41.0
37	35.43486689999814	38.0	35.0	40.0	27.0	41.0
38	35.350573325826026	38.0	34.0	40.0	27.0	41.0
39	35.30285105765707	38.0	35.0	40.0	27.0	41.0
40	35.20877323604085	38.0	34.0	40.0	27.0	41.0
41	35.22740194268736	38.0	34.0	40.0	27.0	41.0
42	35.058712359487224	37.0	34.0	40.0	26.0	41.0
43	35.03007528618168	37.0	34.0	40.0	26.0	41.0
44	34.80462991799024	37.0	34.0	40.0	25.0	41.0
45	34.64033678380677	37.0	34.0	40.0	24.0	41.0
46	34.69290511438673	37.0	34.0	40.0	25.0	41.0
47	34.58411860993838	37.0	34.0	40.0	24.0	41.0
48	34.564497866398824	37.0	34.0	40.0	24.0	41.0
49	34.499368547549366	36.0	34.0	40.0	24.0	41.0
50	33.86085771661019	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	6.0
14	6.0
15	29.0
16	83.0
17	210.0
18	511.0
19	1097.0
20	1875.0
21	3159.0
22	4746.0
23	7001.0
24	10578.0
25	15636.0
26	21425.0
27	25442.0
28	27522.0
29	30296.0
30	35302.0
31	42457.0
32	52820.0
33	67497.0
34	111487.0
35	197737.0
36	103031.0
37	126286.0
38	191340.0
39	377000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.870084415946014	25.616001286966778	29.04839393833147	14.46552035875574
2	31.845965476016154	26.60755694243642	28.249195817632955	13.29728176391447
3	27.787620232052763	26.680292564948164	31.61359647404101	13.918490728958059
4	24.395239047892076	29.158184854353447	31.07241044340543	15.374165654349047
5	23.060217258142025	31.977480831276043	30.53225563615139	14.430046274430541
6	20.22428421066381	40.67550631349328	27.361243737896018	11.73896573794689
7	85.70236280776003	3.82027013927703	8.088641211948715	2.388725841014229
8	85.00944944358623	3.2864401687631437	7.334473178911069	4.369637208739549
9	78.20722502600401	5.549081764326958	11.726866050270077	4.5168271593989475
10	42.00358453247425	30.314804999095962	15.008562591478109	12.673047876951676
11	35.77967018726329	22.75580011590951	27.810788384024836	13.653741312802364
12	33.963892057036276	21.010351420303962	27.60255000917789	17.42320651348187
13	21.39699144015845	34.54027717909531	29.285987805439774	14.776743575306462
14	16.298141804214676	37.143978721049265	28.82791837935683	17.729961095379227
15	14.725663643807193	25.022979094352127	44.23638939819866	16.014967863642017
16	15.823847796928742	21.55703730897446	43.667841573839375	18.95127332025742
17	16.36585880626956	23.41818926798952	30.34890411891243	29.867047806828484
18	20.465315489043938	24.151732833396238	33.62654451481971	21.756407162740114
19	24.196694172831666	29.145947670225762	26.79950198785494	19.857856169087633
20	27.32549466066907	25.592008156289463	25.909281216678593	21.173215966362868
21	21.842823682113703	31.170376664652345	26.645643459328205	20.341156193905746
22	20.672316395833032	26.818476498075395	26.082183003651217	26.427024102440356
23	18.44006151591212	32.34205267076543	25.130707563611015	24.08717824971143
24	19.658830056449165	25.982154335641212	34.712285239137266	19.646730368772356
25	18.43951153010863	27.517439706087586	31.4941120582325	22.548936705571286
26	17.709474124199172	32.87746385046436	28.72603350926004	20.687028516076428
27	20.302657187661342	29.786337390166118	28.304056901531226	21.60694852064131
28	18.044140485623714	28.91735982064963	34.60380053939858	18.43469915432808
29	18.286065490934515	24.750461128722115	33.60805124217731	23.35542213816606
30	21.900434695029432	27.339519298658104	30.223301111040072	20.536744895272392
31	25.254798110523772	26.27302807746275	26.358344625229364	22.113829186784116
32	28.2287088464529	26.279009173075718	25.928324475124487	19.56395750534689
33	25.364589026545755	26.82218890224896	26.74147848558659	21.071743585618698
34	20.19740365451817	25.857788795826707	30.819210729123053	23.12559682053207
35	21.719145624553565	25.148238361097302	31.610709048572684	21.521906965776445
36	26.14220020445722	24.99712976158803	27.92766036726677	20.93300966668798
37	22.77525586370802	27.540195368707042	30.32649219742015	19.358056570164784
38	20.127486709249318	30.215945050918375	27.579106864304066	22.077461375528245
39	22.14263469324198	28.893779179324934	27.5191584117235	21.444427715709587
40	24.16747617702118	26.271653112954024	27.605368686420782	21.955502023604016
41	19.041539740255455	24.446731468743963	28.822212276645608	27.689516514354974
42	23.317335621274278	27.269327360487505	25.871950930266614	23.541386087971606
43	22.90216508786367	26.225935543038798	27.90029857354307	22.971600795554465
44	22.396728134455028	29.97140761304099	27.293526735841134	20.338337516662854
45	19.43746077054386	30.2135388630281	26.440292509949586	23.908707856478454
46	23.24597496327126	28.59960552268245	26.698510844688823	21.455908669357473
47	22.20354562097866	25.646456750835117	28.703277846640585	23.44671978154564
48	23.634058695859913	22.465476359891458	29.450639805360023	24.449825138888603
49	21.69982737320593	22.286180987953248	30.007569179620553	26.006422459220268
50	19.57310101932994	27.240796846931385	29.791287262397535	23.394814871341133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1268.0
1	1210.5
2	1153.0
3	6089.0
4	11025.0
5	8099.5
6	5174.0
7	5631.0
8	6088.0
9	6761.5
10	7435.0
11	7854.0
12	8273.0
13	8229.0
14	8185.0
15	7877.5
16	7570.0
17	7325.0
18	7080.0
19	7046.0
20	7012.0
21	6780.0
22	6548.0
23	7073.5
24	7599.0
25	8443.5
26	9288.0
27	10356.5
28	11425.0
29	14066.0
30	16707.0
31	19428.0
32	22149.0
33	25426.0
34	28703.0
35	33077.5
36	37452.0
37	40612.5
38	43773.0
39	53843.0
40	63913.0
41	81798.0
42	99683.0
43	116049.5
44	132416.0
45	141876.5
46	151337.0
47	160697.0
48	170057.0
49	164820.0
50	159583.0
51	139765.0
52	119947.0
53	102007.0
54	84067.0
55	70063.5
56	56060.0
57	51399.0
58	46738.0
59	41295.0
60	35852.0
61	32346.0
62	28840.0
63	24639.0
64	20438.0
65	15943.5
66	11449.0
67	9199.5
68	6950.0
69	5928.0
70	4906.0
71	3672.5
72	2439.0
73	2460.5
74	2482.0
75	2018.0
76	1554.0
77	1377.5
78	1201.0
79	785.5
80	370.0
81	279.5
82	189.0
83	114.5
84	40.0
85	68.0
86	96.0
87	73.5
88	51.0
89	31.5
90	12.0
91	6.5
92	1.0
93	3.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1454583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.2889002969383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18649485736472	21.336234627297053
2	7.822016807132355	4.269084735416209
3	2.8036081352488638	2.29522148623474
4	1.5342617571374875	1.6747326447972104
5	0.9955841763582095	1.358419866292431
6	0.7438938318223713	1.2180026764864844
7	0.6003010683190146	1.1467089201052196
8	0.48245067310831236	1.0532438653314806
9	0.3714858449733897	0.9123696166682438
>10	4.95183537776546	33.24247531018107
>50	1.3884189865287797	25.017657779235396
>100	0.11381815557632044	4.2847199130606946
>500	0.0025349255064139806	0.46319800569385067
>1k	0.0030419106076967767	1.2416279874849052
>5k	2.5349255064139806E-4	0.4863025657150195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7030	0.4833000248181094	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2566	0.17640794646988173	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	1737	0.11941566758308052	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	1736	0.11934691935764409	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1673	0.11501578115514893	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	1502	0.10325983460551925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1273904617337065	0.0
2	0.0	0.0	0.0	0.45559448996722773	0.0
3	0.0	0.0	0.0	0.7961731987793065	0.0
4	0.0	0.0	0.0	1.2787169931176152	0.0
5	0.0	0.0	0.0	2.0426472741672357	0.0
6	0.0	0.0	0.0	3.69391090092487	0.0
7	0.0	0.0	0.0	4.559451059169535	0.0
8	0.0	0.0	0.0	7.106091574011246	0.0
9	0.0	0.0	0.0	8.528629854741874	0.0
10	0.0	0.0	0.0	10.409168813330005	0.0
11	0.0	0.0	0.0	11.498278200693944	0.0
12	0.0	0.0	0.0	12.467284438220439	0.0
13	0.0	0.0	0.0	13.02799496487997	0.0
14	0.0	0.0	0.0	13.286832033648132	0.0
15	0.0	0.0	0.0	13.512119968403315	0.0
16	0.0	0.0	0.0	13.909828452553068	0.0
17	0.0	0.0	0.0	14.313036794737735	0.0
18	0.0	0.0	0.0	14.918639912607256	0.0
19	0.0	0.0	0.0	15.187926711641756	0.0
20	0.0	0.0	0.0	15.519568151147098	0.0
21	0.0	0.0	0.0	15.838284924270392	0.0
22	0.0	0.0	0.0	16.17315753037125	0.0
23	0.0	0.0	0.0	16.55952255732399	0.0
24	0.0	0.0	0.0	16.83857160437046	0.0
25	0.0	0.0	0.0	17.084690251432885	0.0
26	0.0	0.0	0.0	17.315271799546675	0.0
27	0.0	0.0	0.0	17.581121187309353	0.0
28	0.0	0.0	0.0	17.822496206816663	0.0
29	0.0	0.0	0.0	18.080783289781333	0.0
30	0.0	0.0	0.0	18.450167505051276	0.0
31	0.0	0.0	0.0	18.71498566943241	0.0
32	0.0	0.0	0.0	18.97286026304446	0.0
33	0.0	0.0	0.0	19.212654073366732	0.0
34	0.0	0.0	0.0	19.474584812279534	0.0
35	0.0	0.0	0.0	19.758446235106558	0.0
36	0.0	0.0	0.0	20.011164711810878	0.0
37	0.0	0.0	0.0	20.27515789748677	0.0
38	0.0	0.0	0.0	20.524163970017526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTGCG	20	7.857171E-4	44.000004	1
TGCGCAA	20	7.857171E-4	44.000004	13
CTACGCG	30	2.5281824E-6	44.000004	1
TTACGAG	40	8.314601E-9	44.000004	1
TACTACG	30	2.5281824E-6	44.000004	1
TCGTTAG	85	0.0	44.0	1
CGTGCCG	25	4.443426E-5	44.0	2
TACGAAG	50	2.7284841E-11	44.0	1
CGTTTTT	6190	0.0	41.5832	1
CGGTCTA	170	0.0	41.411766	31
ACGGGAT	645	0.0	39.224804	5
CGACGGT	180	0.0	39.11111	28
GACCGAC	45	2.3515895E-8	39.11111	9
CGAGGGA	855	0.0	38.8538	4
ATTACGG	85	0.0	38.823532	2
TATTACG	40	4.1255407E-7	38.500004	1
CCGCATC	40	4.1255407E-7	38.500004	20
CGACAGG	155	0.0	38.322582	2
TAGGGAC	1285	0.0	38.17899	5
GCTACGA	220	0.0	38.0	10
>>END_MODULE