FastQCFastQC Report
Thu 26 May 2016
SRR1547247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547247_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2630668
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216160.8216924370540106No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32280.12270647607375768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA254.444409E-544.027
CACGACG5400.042.3703726
CGTTTTT150750.042.1174131
CGGTCTA5400.041.96296331
CGACGGT5500.040.828
TCACGAC5650.040.49557525
TAGGGCG12150.039.473255
ACGGGTA2400.039.4166645
ACGGTCT5700.039.36842330
TAGGGTA14300.038.769235
CTCACGA5850.038.73504324
AATAGCG1600.038.51
TAAGGGA29700.038.370374
AAATGCG1950.038.3589741
GCGTAAG1550.038.3225821
TGCGTAG1500.038.133331
GACGGTC5900.038.03389729
ATAACGC2850.037.82456211
GTTTTTT173300.037.7287942
TATCGTG357.293789E-637.7142871
Produced by FastQC (version 0.11.5)