##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547247_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2630668
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300904941254466	31.0	31.0	33.0	30.0	34.0
2	31.69556401643993	31.0	31.0	34.0	30.0	34.0
3	31.810000729852646	31.0	31.0	34.0	30.0	34.0
4	35.50303991229604	37.0	35.0	37.0	33.0	37.0
5	35.380498413330756	37.0	35.0	37.0	33.0	37.0
6	35.41980439949093	37.0	35.0	37.0	33.0	37.0
7	35.95241322736278	37.0	35.0	37.0	35.0	37.0
8	35.944501928787666	37.0	35.0	37.0	35.0	37.0
9	37.64130441393593	39.0	37.0	39.0	35.0	39.0
10	36.972667018415095	39.0	37.0	39.0	32.0	39.0
11	36.73618487775728	39.0	35.0	39.0	32.0	39.0
12	36.29072653789836	38.0	35.0	39.0	32.0	39.0
13	36.12333407332282	38.0	35.0	39.0	32.0	39.0
14	37.17964600626153	39.0	35.0	41.0	32.0	41.0
15	37.364211295382006	39.0	35.0	41.0	32.0	41.0
16	37.418519934860655	39.0	35.0	41.0	32.0	41.0
17	37.34226857969155	39.0	35.0	41.0	32.0	41.0
18	37.28033868203818	39.0	35.0	41.0	32.0	41.0
19	37.21581590683431	39.0	35.0	41.0	32.0	41.0
20	37.02871666055922	39.0	35.0	41.0	32.0	41.0
21	36.91883392355098	38.0	35.0	41.0	31.0	41.0
22	36.84853618928728	38.0	35.0	41.0	31.0	41.0
23	36.755950199721134	38.0	35.0	41.0	31.0	41.0
24	36.68678791850587	38.0	35.0	41.0	31.0	41.0
25	36.49494577042789	38.0	35.0	40.0	31.0	41.0
26	36.380225478851756	38.0	35.0	40.0	31.0	41.0
27	36.27519246062217	38.0	35.0	40.0	30.0	41.0
28	36.34017253412441	38.0	35.0	40.0	30.0	41.0
29	36.271659137527045	38.0	35.0	40.0	30.0	41.0
30	36.243698558693076	38.0	35.0	40.0	30.0	41.0
31	36.11659167937573	38.0	35.0	40.0	30.0	41.0
32	35.85120889447091	38.0	35.0	40.0	29.0	41.0
33	35.72619539979959	38.0	35.0	40.0	29.0	41.0
34	35.59393773748721	38.0	35.0	40.0	28.0	41.0
35	35.3951984819065	38.0	35.0	40.0	27.0	41.0
36	35.21507008866189	38.0	34.0	40.0	25.0	41.0
37	35.171580374262355	38.0	34.0	40.0	26.0	41.0
38	35.12253427646514	38.0	34.0	40.0	26.0	41.0
39	35.02096387685561	38.0	34.0	40.0	25.0	41.0
40	34.91223940079098	38.0	34.0	40.0	24.0	41.0
41	34.89117288840705	37.0	34.0	40.0	24.0	41.0
42	34.68887256012541	37.0	34.0	40.0	24.0	41.0
43	34.7098371972442	37.0	34.0	40.0	24.0	41.0
44	34.44006997462242	37.0	34.0	40.0	23.0	41.0
45	34.324892384747905	37.0	33.0	40.0	23.0	41.0
46	34.30810387323676	36.0	33.0	40.0	23.0	41.0
47	34.190612422396136	36.0	33.0	40.0	23.0	41.0
48	34.14794227169677	36.0	33.0	40.0	23.0	41.0
49	34.13953071995402	36.0	33.0	40.0	23.0	41.0
50	33.595493235938555	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	5.0
13	12.0
14	31.0
15	69.0
16	207.0
17	532.0
18	1220.0
19	2450.0
20	4260.0
21	6909.0
22	10193.0
23	14983.0
24	21530.0
25	30749.0
26	42958.0
27	51299.0
28	55067.0
29	59096.0
30	67489.0
31	81074.0
32	99223.0
33	125736.0
34	196895.0
35	305127.0
36	203536.0
37	253367.0
38	368017.0
39	628630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.929912858635145	24.20480273451458	28.323338406822906	15.541946000027371
2	33.34415441249143	25.074239698814143	28.51195969996974	13.06964618872469
3	28.338999828180523	25.149163634483713	33.1586882115113	13.353148325824469
4	25.041320303436237	27.668599762493784	32.43761660536411	14.852463328705864
5	22.08876224593905	32.03254838694963	31.281864530225782	14.596824836885535
6	19.27544638852185	40.88353224352142	28.921893602689508	10.919127765267225
7	85.05938415641958	3.7755429419447837	8.958675134984727	2.2063977666509036
8	84.59303872628549	3.209831115138816	8.57690898281349	3.620221175762202
9	78.97819109062793	5.558854252988215	11.593215107341557	3.869739549042296
10	39.000360364743855	31.805191685153734	16.05485754948933	13.139590400613077
11	34.45767386838628	24.051115534153304	26.206917786661034	15.284292810799386
12	31.677923630043775	21.331578139088627	28.31721828828267	18.673279942584927
13	22.653979901682767	29.84234422587723	30.064835243367842	17.43884062907216
14	18.21491727576418	33.24258325261873	29.831814580935333	18.710684890681758
15	17.14420063649233	23.519957668546542	40.70540258215784	18.630439112803288
16	18.640246507731117	21.544907985348207	39.26516002779522	20.549685479125454
17	18.211610130962935	22.447834542405197	31.32767038638095	28.012884940250913
18	21.764015831720307	23.65422014484534	32.531699172985725	22.05006485044863
19	25.388532494408267	27.143105857523643	26.895716221127103	20.572645426940987
20	27.398934415137145	24.61051717662586	26.240825524163448	21.74972288407355
21	23.206463149283756	28.64295304462593	27.407335323195475	20.74324848289484
22	22.424646515637853	24.653662111676578	26.735680823273782	26.186010549411787
23	20.857249945641183	29.66349231449959	25.781816633645903	23.697441106213326
24	20.98216118491577	25.328547730082246	33.20913167301993	20.48015941198205
25	20.826763392415916	26.13955846955982	30.641532873019322	22.392145265004935
26	19.210862032000996	29.858461805138464	28.258754050302056	22.67192211255848
27	21.72208731774591	27.16321481844155	27.997451597845107	23.117246265967427
28	19.807098425190865	27.13257621258175	33.34191163613197	19.718413726095424
29	20.278195500154332	24.5350610567354	31.662642340272505	23.524101102837758
30	22.427535515694114	26.492054489582113	29.894004108462184	21.18640588626159
31	26.729294612623107	25.00174860529721	25.032235158522475	23.23672162355721
32	27.541901904763357	25.678154750048275	25.866433924767396	20.913509420420972
33	24.342182289821444	25.613494367210155	25.48827902266649	24.556044320301915
34	20.554285071320287	26.99169944668046	29.142750054358817	23.311265427640432
35	20.5247488470609	25.700848605753368	29.796576382880698	23.977826164305036
36	24.908160208737858	27.880257029773425	26.080486021041043	21.131096740447674
37	20.806198273594386	31.036527604395538	27.37772307261882	20.779551049391255
38	20.19851991965539	31.081801276329813	25.6511273942588	23.068551409756
39	20.773126825581944	30.647919083670004	25.188393214195028	23.390560876553028
40	22.836671142082544	26.573022517474648	27.235173727737592	23.355132612705216
41	19.420808707142065	26.008755190696814	27.621273379993216	26.94916272216791
42	22.726318942565157	26.957373564433063	25.04641406669333	25.26989342630845
43	22.158896523620616	26.058172296922304	27.075100316725635	24.70783086273144
44	22.114953312238566	28.054281270004427	27.73755563225766	22.093209785499347
45	19.919845453702255	29.287960320344492	26.96353169613193	23.828662529821322
46	23.756665607366646	28.281675984958955	26.015103388188855	21.946555019485544
47	22.47402560870471	25.824809516062082	27.70303968421709	23.998125191016122
48	22.976673605335225	24.306335881228648	28.2674970767881	24.44949343664803
49	22.116321785949424	24.39330998818551	28.206409930861664	25.2839582950034
50	20.463281569548116	26.54675542485787	29.248730740633178	23.74123226496084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2844.0
1	3038.0
2	3232.0
3	18346.0
4	33460.0
5	23444.5
6	13429.0
7	13886.5
8	14344.0
9	15192.5
10	16041.0
11	16247.5
12	16454.0
13	16044.5
14	15635.0
15	15145.0
16	14655.0
17	14371.0
18	14087.0
19	13262.5
20	12438.0
21	11879.5
22	11321.0
23	11755.0
24	12189.0
25	13728.5
26	15268.0
27	16709.0
28	18150.0
29	22644.0
30	27138.0
31	30019.0
32	32900.0
33	40493.5
34	48087.0
35	53480.0
36	58873.0
37	65520.0
38	72167.0
39	90444.0
40	108721.0
41	135770.0
42	162819.0
43	194475.5
44	226132.0
45	248509.5
46	270887.0
47	263482.0
48	256077.0
49	234910.5
50	213744.0
51	193027.5
52	172311.0
53	158511.5
54	144712.0
55	135651.5
56	126591.0
57	119072.0
58	111553.0
59	104021.0
60	96489.0
61	87597.5
62	78706.0
63	70744.5
64	62783.0
65	52603.0
66	42423.0
67	37859.5
68	33296.0
69	29933.0
70	26570.0
71	22454.0
72	18338.0
73	15064.0
74	11790.0
75	9250.5
76	6711.0
77	5505.5
78	4300.0
79	3001.0
80	1702.0
81	1200.5
82	699.0
83	530.0
84	361.0
85	266.0
86	171.0
87	102.0
88	33.0
89	21.5
90	10.0
91	10.5
92	11.0
93	9.5
94	8.0
95	7.5
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2630668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.57704657244162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48546718849754	20.074264495400403
2	7.632107502638448	3.9041353808172934
3	2.8524546001564652	2.188720924619317
4	1.506928277622823	1.541710989523527
5	0.9729350517149901	1.2442402564837596
6	0.7409170414807446	1.1370281805761198
7	0.5616804838947932	1.0056289526786069
8	0.4931338198840342	1.0090325342095985
9	0.40791190457033155	0.9389863602583858
>10	4.657616710403981	27.879388042260533
>50	1.3121429566686234	23.57129435811063
>100	0.3695211711672434	12.877655621906964
>500	0.004639208964585879	0.8498539441817989
>1k	0.002394430433334647	0.950674341829633
>5k	0.0	0.0
>10k+	1.4965190208341545E-4	0.8273856171433485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21616	0.8216924370540106	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3228	0.12270647607375768	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07712869887040098	0.0
2	0.0	0.0	0.0	0.2818295581198388	0.0
3	0.0	0.0	0.0	0.5035983255963884	0.0
4	0.0	0.0	0.0	0.7696524228827051	0.0
5	0.0	0.0	0.0	1.281081459157902	0.0
6	3.801315863499309E-5	0.0	0.0	2.3360986639135004	0.0
7	3.801315863499309E-5	0.0	0.0	3.009805874401483	0.0
8	3.801315863499309E-5	0.0	0.0	4.937339109306078	0.0
9	3.801315863499309E-5	0.0	0.0	6.05040240729731	0.0
10	3.801315863499309E-5	0.0	0.0	7.523260251768752	0.0
11	3.801315863499309E-5	0.0	0.0	8.262312081950288	0.0
12	3.801315863499309E-5	0.0	0.0	9.029569675838989	0.0
13	3.801315863499309E-5	0.0	0.0	9.439503578558753	0.0
14	3.801315863499309E-5	0.0	0.0	9.630291621747784	0.0
15	3.801315863499309E-5	0.0	0.0	9.797131374996768	0.0
16	3.801315863499309E-5	0.0	0.0	10.090326867548471	0.0
17	3.801315863499309E-5	0.0	0.0	10.39872762355417	0.0
18	3.801315863499309E-5	0.0	0.0	10.914946317817376	0.0
19	3.801315863499309E-5	0.0	0.0	11.113755137478389	0.0
20	3.801315863499309E-5	0.0	0.0	11.367226879256524	0.0
21	3.801315863499309E-5	0.0	0.0	11.605835476008375	0.0
22	3.801315863499309E-5	0.0	0.0	11.85413742821215	0.0
23	3.801315863499309E-5	0.0	0.0	12.143873723328067	0.0
24	3.801315863499309E-5	0.0	0.0	12.365186332900997	0.0
25	3.801315863499309E-5	0.0	0.0	12.551336770736558	0.0
26	3.801315863499309E-5	0.0	0.0	12.741668656021968	0.0
27	3.801315863499309E-5	0.0	0.0	12.94914447585176	0.0
28	3.801315863499309E-5	0.0	0.0	13.146584821801914	0.0
29	3.801315863499309E-5	0.0	0.0	13.356075339039363	0.0
30	3.801315863499309E-5	0.0	0.0	13.64953692370151	0.0
31	3.801315863499309E-5	0.0	0.0	13.880961033471346	0.0
32	3.801315863499309E-5	0.0	0.0	14.11303136693798	0.0
33	3.801315863499309E-5	0.0	0.0	14.327615647432514	0.0
34	3.801315863499309E-5	0.0	0.0	14.543454362162006	0.0
35	3.801315863499309E-5	0.0	0.0	14.79008373538584	0.0
36	3.801315863499309E-5	0.0	0.0	15.003679673755867	0.0
37	3.801315863499309E-5	0.0	0.0	15.231530546614016	0.0
38	3.801315863499309E-5	0.0	0.0	15.466299814343733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	4.444409E-5	44.0	27
CACGACG	540	0.0	42.37037	26
CGTTTTT	15075	0.0	42.117413	1
CGGTCTA	540	0.0	41.962963	31
CGACGGT	550	0.0	40.8	28
TCACGAC	565	0.0	40.495575	25
TAGGGCG	1215	0.0	39.47325	5
ACGGGTA	240	0.0	39.416664	5
ACGGTCT	570	0.0	39.368423	30
TAGGGTA	1430	0.0	38.76923	5
CTCACGA	585	0.0	38.735043	24
AATAGCG	160	0.0	38.5	1
TAAGGGA	2970	0.0	38.37037	4
AAATGCG	195	0.0	38.358974	1
GCGTAAG	155	0.0	38.322582	1
TGCGTAG	150	0.0	38.13333	1
GACGGTC	590	0.0	38.033897	29
ATAACGC	285	0.0	37.824562	11
GTTTTTT	17330	0.0	37.728794	2
TATCGTG	35	7.293789E-6	37.714287	1
>>END_MODULE