FastQCFastQC Report
Thu 26 May 2016
SRR1547246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547246_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences685104
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35500.5181695041920642No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8960.13078306359326466No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC7200.10509353324458769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1300.044.00000428
TCACGCC207.854253E-444.034
TACCCGA207.854253E-444.040
CAACCCG207.854253E-444.023
ATTAACG207.854253E-444.01
TGACGTA207.854253E-444.010
TTATACG207.854253E-444.01
CTCACGA1400.042.42857424
GACGGTC1350.042.37037329
CGTTTTT22450.041.9420931
GCGTTAG800.041.251
AATACGG650.040.6153872
ACGGGTA557.8216544E-1140.05
TCACGAC1500.039.625
CAGCGAT1000.039.621
GTATGCG501.3460522E-939.61
GCGTAAG501.3460522E-939.61
CGGTCTA1450.039.44827331
TAGGGTA3200.039.18755
TGTAGCG452.3484972E-839.111111
Produced by FastQC (version 0.11.5)