##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547246_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	685104
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462332726126252	31.0	31.0	33.0	30.0	34.0
2	31.85173054018076	31.0	31.0	34.0	30.0	34.0
3	31.98183633433756	33.0	31.0	34.0	30.0	34.0
4	35.642587110862	37.0	35.0	37.0	33.0	37.0
5	35.52920870407996	37.0	35.0	37.0	33.0	37.0
6	35.56630088278568	37.0	35.0	37.0	33.0	37.0
7	36.027627922184074	37.0	35.0	37.0	35.0	37.0
8	36.02602086690488	37.0	35.0	37.0	35.0	37.0
9	37.75162749013288	39.0	38.0	39.0	35.0	39.0
10	37.123150645741376	39.0	37.0	39.0	33.0	39.0
11	36.91945748382727	39.0	37.0	39.0	33.0	39.0
12	36.31149577290455	38.0	35.0	39.0	32.0	39.0
13	36.049909210864335	38.0	35.0	39.0	32.0	39.0
14	37.10582188981527	39.0	35.0	41.0	32.0	41.0
15	37.32689197552489	39.0	35.0	41.0	32.0	41.0
16	37.41089382050024	39.0	35.0	41.0	33.0	41.0
17	37.34726114575305	39.0	35.0	41.0	32.0	41.0
18	37.322377332492586	39.0	35.0	41.0	32.0	41.0
19	37.28694037693547	39.0	35.0	41.0	32.0	41.0
20	37.12971315303954	39.0	35.0	41.0	32.0	41.0
21	37.01037068824587	39.0	35.0	41.0	32.0	41.0
22	36.93476026997361	38.0	35.0	41.0	32.0	41.0
23	36.868536747705456	38.0	35.0	41.0	32.0	41.0
24	36.78849780471286	38.0	35.0	41.0	32.0	41.0
25	36.635103575515544	38.0	35.0	41.0	31.0	41.0
26	36.52031078493192	38.0	35.0	40.0	31.0	41.0
27	36.4303857516523	38.0	35.0	40.0	31.0	41.0
28	36.526981305028144	38.0	35.0	40.0	31.0	41.0
29	36.47267130245919	38.0	35.0	40.0	31.0	41.0
30	36.468433989584064	38.0	35.0	40.0	31.0	41.0
31	36.36108094537472	38.0	35.0	40.0	31.0	41.0
32	36.18745475139541	38.0	35.0	40.0	30.0	41.0
33	36.130956759849596	38.0	35.0	40.0	30.0	41.0
34	36.06446028632149	38.0	35.0	40.0	30.0	41.0
35	35.89134058478713	38.0	35.0	40.0	30.0	41.0
36	35.75422271655106	38.0	35.0	40.0	29.0	41.0
37	35.70698755225484	38.0	35.0	40.0	29.0	41.0
38	35.65948089633107	38.0	35.0	40.0	29.0	41.0
39	35.58407044769845	38.0	35.0	40.0	29.0	41.0
40	35.46402444008501	37.0	35.0	40.0	29.0	41.0
41	35.458793117541276	37.0	35.0	40.0	29.0	41.0
42	35.25679166958593	37.0	34.0	40.0	28.0	41.0
43	35.273076788341626	37.0	34.0	40.0	28.0	41.0
44	34.94032146944114	37.0	34.0	40.0	27.0	41.0
45	34.865980931362245	36.0	34.0	40.0	27.0	41.0
46	34.89652082019664	36.0	34.0	40.0	27.0	41.0
47	34.79527779723954	36.0	34.0	40.0	27.0	41.0
48	34.77648940890726	36.0	34.0	40.0	27.0	41.0
49	34.76193541418529	36.0	34.0	40.0	27.0	41.0
50	34.240239438099906	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	8.0
15	12.0
16	43.0
17	79.0
18	203.0
19	434.0
20	774.0
21	1315.0
22	1887.0
23	2984.0
24	4257.0
25	6087.0
26	8436.0
27	10414.0
28	11790.0
29	13398.0
30	15835.0
31	19570.0
32	24494.0
33	32846.0
34	54804.0
35	93631.0
36	52413.0
37	64367.0
38	94910.0
39	170111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.069431794296925	24.46124967888087	28.525012260912213	13.944306265909992
2	32.26342277960718	24.975186249095028	29.305331745253277	13.45605922604451
3	29.084927251920877	25.765285270557463	31.439606249562107	13.71018122795955
4	26.34534318877134	28.1717812186179	30.584699549265515	14.898176043345243
5	23.16947499941615	32.54411009131461	29.251617272706042	15.034797636563207
6	19.851584576940144	41.422470165113616	27.347672762091594	11.378272495854644
7	87.398117658049	4.049896074172681	6.603668932016162	1.9483173357621615
8	87.34206777365189	3.403862771199701	6.10038767836708	3.1536817767813354
9	82.12227632593007	5.263872346388285	9.002574791564493	3.6112765361171446
10	43.95580816927065	27.253380508652704	14.481596954622947	14.309214367453702
11	39.49882061701581	23.58503234545412	21.667513253462246	15.248633784067819
12	35.73778579602513	20.89390807818959	25.50386510661155	17.864441019173732
13	23.48242018730003	32.67810434620145	26.675803965529322	17.163671500969198
14	18.225700039702	36.08371867628856	27.083333333333332	18.6072479506761
15	16.78956771526659	22.875359069571918	42.272122188747986	18.062951026413508
16	18.558934118031715	20.001050935332447	40.96852448679324	20.47149045984259
17	18.391806207524695	21.084244143954788	28.613757911207642	31.910191737312875
18	22.129049020294726	23.32930474789229	31.71372521544174	22.827921016371235
19	27.54530698988767	26.15559097596861	24.828201265793222	21.470900768350496
20	29.873858567458374	23.792738036852796	23.72544898292814	22.60795441276069
21	24.64034657511852	28.02859127957215	25.543275181578274	21.787786963731055
22	23.857253789205725	24.872428127700317	24.40797309605549	26.862344987038465
23	21.677146827343	29.98420677736519	23.733330997921485	24.605315397370326
24	21.47148462131297	25.386802587636332	32.22970527102454	20.912007520026158
25	21.84354492164693	24.539777902333075	30.46530161844041	23.151375557579577
26	19.307141689436932	30.800579182138772	26.294693944277075	23.597585184147224
27	21.365661271865292	28.43276349284196	27.566325696536587	22.63524953875616
28	19.24642098134006	27.356722483009875	33.87120787500876	19.525648660641302
29	19.762692963404096	25.082615194189493	32.10928559751512	23.045406244891286
30	21.944405520913612	26.783378873864404	29.88553562670777	21.386679978514213
31	27.633906676942477	25.288423363460144	23.833461780985076	23.2442081786123
32	27.85577080268105	26.12274924682968	25.8382668908662	20.183213059623064
33	25.651725869357062	26.709667437352575	25.0275870524767	22.611019640813655
34	20.568701978093838	26.727474952707908	28.915755856045212	23.788067213153038
35	22.27968308461197	24.658591980195705	29.247530301968755	23.81419463322357
36	27.890510053948013	24.67873490740092	25.419644316775265	22.011110721875802
37	22.39864312571522	28.769500688946497	27.318626077208712	21.51323010812957
38	21.908936453443566	29.305477708493893	24.45541114925617	24.330174688806373
39	21.607522361568464	28.45611761134076	25.556849762955697	24.379510264135078
40	23.432792685490085	24.710262967374298	26.562244564328918	25.294699782806696
41	18.761968985730633	24.26887012774703	28.024796235316096	28.94436465120624
42	23.094449893738762	27.014000794040026	24.413081809477102	25.478467502744106
43	22.906741166304677	26.619900044372823	26.012838926644715	24.46051986267778
44	22.513954085803032	28.681484854854155	27.282719120016814	21.521841939326
45	20.568993904575073	29.699578458161096	25.213690184263992	24.517737452999835
46	23.706035871926016	27.91109087087508	26.18522151381396	22.197651743384945
47	23.284056143300873	25.85709614890586	26.816950419206425	24.041897288586842
48	23.518181181251315	24.134875872860178	27.676965832924637	24.66997711296387
49	21.953017352110045	23.92833788738644	28.734615474438918	25.384029286064596
50	20.34333473458044	27.311473878418457	28.1592283799248	24.185963007076296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	799.0
1	838.0
2	877.0
3	3386.0
4	5895.0
5	4143.5
6	2392.0
7	2502.5
8	2613.0
9	2819.5
10	3026.0
11	3016.0
12	3006.0
13	2900.0
14	2794.0
15	2693.0
16	2592.0
17	2425.5
18	2259.0
19	2310.5
20	2362.0
21	2364.0
22	2366.0
23	2393.5
24	2421.0
25	2860.5
26	3300.0
27	3992.0
28	4684.0
29	5338.0
30	5992.0
31	6900.5
32	7809.0
33	9560.0
34	11311.0
35	12907.0
36	14503.0
37	15832.0
38	17161.0
39	21505.5
40	25850.0
41	34465.0
42	43080.0
43	51375.5
44	59671.0
45	64131.0
46	68591.0
47	65567.0
48	62543.0
49	58902.0
50	55261.0
51	51410.5
52	47560.0
53	44087.0
54	40614.0
55	38184.5
56	35755.0
57	34210.5
58	32666.0
59	31757.0
60	30848.0
61	28140.0
62	25432.0
63	22489.0
64	19546.0
65	16147.0
66	12748.0
67	10879.5
68	9011.0
69	7929.5
70	6848.0
71	5529.5
72	4211.0
73	3597.0
74	2983.0
75	2411.0
76	1839.0
77	1404.0
78	969.0
79	767.5
80	566.0
81	401.0
82	236.0
83	160.0
84	84.0
85	49.5
86	15.0
87	13.0
88	11.0
89	6.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	685104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.51258495014093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53577654076587	21.252076604433082
2	6.559676734685234	3.740666802863516
3	2.8860084292571435	2.4686268151805124
4	1.8348264726230037	2.0926258267772333
5	1.356268488785651	1.9335360250850069
6	1.1361197183856935	1.9436225990401366
7	0.9035482388708536	1.8033747142148806
8	0.7743940204438898	1.7663980234230066
9	0.7074856769880749	1.8155020919517404
>10	9.042710103297635	54.46585808253248
>50	0.22507259621723047	4.022403776272849
>100	0.0355377783500454	1.764858843532463
>500	0.0020601610637707483	0.4091277032851923
>1k	5.150402659426871E-4	0.5213220914079084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3550	0.5181695041920642	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	896	0.13078306359326466	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	720	0.10509353324458769	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.057801443284523225	0.0
2	0.0	0.0	0.0	0.2406933837782293	0.0
3	0.0	0.0	0.0	0.4213958756626731	0.0
4	0.0	0.0	0.0	0.6191760666993624	0.0
5	0.0	0.0	0.0	0.956059226044513	0.0
6	0.0	0.0	0.0	1.7013475326373806	0.0
7	0.0	0.0	0.0	2.1417186295803265	0.0
8	0.0	0.0	0.0	3.5854410425278496	0.0
9	0.0	0.0	0.0	4.412176837385273	0.0
10	0.0	0.0	0.0	5.5020843550760175	0.0
11	0.0	0.0	0.0	6.096446670870408	0.0
12	0.0	0.0	0.0	6.663367897428712	0.0
13	0.0	0.0	0.0	6.951207407926388	0.0
14	0.0	0.0	0.0	7.084909736332002	0.0
15	0.0	0.0	0.0	7.204891520119573	0.0
16	0.0	0.0	0.0	7.4251500502113545	0.0
17	0.0	0.0	0.0	7.659567014643033	0.0
18	0.0	0.0	0.0	8.072642985590509	0.0
19	0.0	0.0	0.0	8.226488241201336	0.0
20	0.0	0.0	0.0	8.426603844087905	0.0
21	0.0	0.0	0.0	8.614458534762605	0.0
22	0.0	0.0	0.0	8.791949835353465	0.0
23	0.0	0.0	0.0	9.015565519979448	0.0
24	0.0	0.0	0.0	9.175103341974356	0.0
25	0.0	0.0	0.0	9.30895163362059	0.0
26	0.0	0.0	0.0	9.441340292860646	0.0
27	0.0	0.0	0.0	9.602191784021112	0.0
28	1.459632406174829E-4	0.0	0.0	9.745819612788715	0.0
29	1.459632406174829E-4	0.0	0.0	9.904773581821154	0.0
30	1.459632406174829E-4	0.0	0.0	10.128681192928372	0.0
31	1.459632406174829E-4	0.0	0.0	10.294641397510452	0.0
32	1.459632406174829E-4	0.0	0.0	10.463082977183026	0.0
33	1.459632406174829E-4	0.0	0.0	10.615176673906443	0.0
34	1.459632406174829E-4	0.0	0.0	10.766832480908008	0.0
35	1.459632406174829E-4	0.0	0.0	10.947826899273688	0.0
36	1.459632406174829E-4	0.0	0.0	11.106342978584273	0.0
37	1.459632406174829E-4	0.0	0.0	11.262815572526215	0.0
38	1.459632406174829E-4	0.0	0.0	11.427316144702118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	130	0.0	44.000004	28
TCACGCC	20	7.854253E-4	44.0	34
TACCCGA	20	7.854253E-4	44.0	40
CAACCCG	20	7.854253E-4	44.0	23
ATTAACG	20	7.854253E-4	44.0	1
TGACGTA	20	7.854253E-4	44.0	10
TTATACG	20	7.854253E-4	44.0	1
CTCACGA	140	0.0	42.428574	24
GACGGTC	135	0.0	42.370373	29
CGTTTTT	2245	0.0	41.942093	1
GCGTTAG	80	0.0	41.25	1
AATACGG	65	0.0	40.615387	2
ACGGGTA	55	7.8216544E-11	40.0	5
TCACGAC	150	0.0	39.6	25
CAGCGAT	100	0.0	39.6	21
GTATGCG	50	1.3460522E-9	39.6	1
GCGTAAG	50	1.3460522E-9	39.6	1
CGGTCTA	145	0.0	39.448273	31
TAGGGTA	320	0.0	39.1875	5
TGTAGCG	45	2.3484972E-8	39.11111	1
>>END_MODULE