FastQCFastQC Report
Thu 26 May 2016
SRR1547245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547245_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1604109
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128100.7985741617309049No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22530.1404518022154355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21700.13527759023856858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC302.5283462E-644.00000420
CGAACCG207.857414E-444.034
GACCGTT254.4436307E-544.010
CAATCGA207.857414E-444.028
TACGTAG1150.042.0869521
CGTTTTT81600.042.031861
CGGTCTA5450.041.9816531
CACGACG5400.041.55555326
TCGATAG700.040.8571431
TCACGAC5650.040.49557525
CGACGGT5600.040.46428728
CAACCCG1650.040.023
CTCACGT1050.039.80952544
ACGTAAG1050.039.8095251
GCGTAAG1350.039.111111
CTCACGA5950.038.4537824
TAGCATA6650.038.3759430
TACGGGA6450.038.201554
GGTACCT7300.037.9726038
TCTCACG6050.037.8181823
Produced by FastQC (version 0.11.5)