##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547245_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1604109
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392559358497458	31.0	31.0	33.0	30.0	34.0
2	31.76828818989233	31.0	31.0	34.0	30.0	34.0
3	31.886345005233434	31.0	31.0	34.0	30.0	34.0
4	35.576095514706296	37.0	35.0	37.0	33.0	37.0
5	35.45330149011071	37.0	35.0	37.0	33.0	37.0
6	35.492155458263746	37.0	35.0	37.0	33.0	37.0
7	35.99473352496619	37.0	35.0	37.0	35.0	37.0
8	35.997121766662985	37.0	35.0	37.0	35.0	37.0
9	37.718677471418715	39.0	37.0	39.0	35.0	39.0
10	37.0887290078168	39.0	37.0	39.0	33.0	39.0
11	36.8156983097782	39.0	37.0	39.0	32.0	39.0
12	36.26371898667734	38.0	35.0	39.0	32.0	39.0
13	36.012372600615045	38.0	35.0	39.0	31.0	39.0
14	37.08171639209056	39.0	35.0	41.0	32.0	41.0
15	37.31133046445098	39.0	35.0	41.0	32.0	41.0
16	37.39080573701663	39.0	35.0	41.0	32.0	41.0
17	37.32765229794234	39.0	35.0	41.0	32.0	41.0
18	37.27338977588181	39.0	35.0	41.0	32.0	41.0
19	37.20999071758839	38.0	35.0	41.0	32.0	41.0
20	37.03653741734508	38.0	35.0	41.0	32.0	41.0
21	36.90819638815068	38.0	35.0	41.0	32.0	41.0
22	36.82807340398938	38.0	35.0	41.0	32.0	41.0
23	36.745701196115725	38.0	35.0	41.0	32.0	41.0
24	36.67707431352857	38.0	35.0	41.0	31.0	41.0
25	36.52433905675986	38.0	35.0	40.0	31.0	41.0
26	36.403463230989914	38.0	35.0	40.0	31.0	41.0
27	36.30592185443757	38.0	35.0	40.0	31.0	41.0
28	36.372705969482126	38.0	35.0	40.0	31.0	41.0
29	36.31618861311794	38.0	35.0	40.0	31.0	41.0
30	36.30088354344998	38.0	35.0	40.0	31.0	41.0
31	36.15641331106552	38.0	35.0	40.0	31.0	41.0
32	35.90220178304592	38.0	35.0	40.0	30.0	41.0
33	35.77391000237515	38.0	35.0	40.0	30.0	41.0
34	35.62023528326317	38.0	35.0	40.0	29.0	41.0
35	35.42840667311261	38.0	35.0	40.0	28.0	41.0
36	35.234871196408726	38.0	34.0	40.0	27.0	41.0
37	35.19095460470579	38.0	34.0	40.0	27.0	41.0
38	35.14256013774625	37.0	34.0	40.0	27.0	41.0
39	35.079451583402374	37.0	34.0	40.0	26.0	41.0
40	34.9637106954702	37.0	34.0	40.0	26.0	41.0
41	34.97375614749372	37.0	34.0	40.0	26.0	41.0
42	34.78450591574513	37.0	34.0	40.0	25.0	41.0
43	34.77559941375555	37.0	34.0	40.0	26.0	41.0
44	34.448642205735396	36.0	34.0	40.0	24.0	41.0
45	34.39886129932567	36.0	34.0	40.0	24.0	41.0
46	34.40390522090456	36.0	34.0	40.0	24.0	41.0
47	34.32190954604706	36.0	33.0	40.0	24.0	41.0
48	34.290483377376475	36.0	34.0	40.0	24.0	41.0
49	34.300153543181914	36.0	34.0	40.0	24.0	41.0
50	33.74005133067641	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	2.0
13	4.0
14	9.0
15	49.0
16	91.0
17	269.0
18	556.0
19	1154.0
20	2170.0
21	3487.0
22	5606.0
23	8305.0
24	11947.0
25	17576.0
26	24065.0
27	28541.0
28	31160.0
29	33731.0
30	39161.0
31	48027.0
32	60472.0
33	78362.0
34	130299.0
35	212802.0
36	125702.0
37	152930.0
38	216543.0
39	371085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56274978820018	23.84357920814608	28.768119872153324	14.825551131500417
2	31.76261712888588	26.034203411364192	28.995785199135472	13.20739426061446
3	27.582227890997434	25.91257825995615	33.114582612528196	13.390611236518216
4	24.74582462912433	28.18630155432081	32.33614423957474	14.731729576980118
5	22.162272015181014	32.184845294178885	31.70414229955695	13.94874039108315
6	19.351677473288913	41.66181973918231	28.074089728316466	10.91241305921231
7	85.02907221392061	4.069112510434141	8.716552304113998	2.185262971531236
8	85.05400817525492	3.2715981270599443	7.950083192601001	3.724310505084131
9	79.33768839898036	5.38666636743513	11.284145902803363	3.9914993307811377
10	44.07692993431244	27.74805203387052	15.291230209418439	12.8837878223986
11	37.32134162952767	22.889716347205834	25.313304769189628	14.475637254076872
12	33.747893690516044	20.934425279080163	27.007516322145193	18.310164708258604
13	22.08141716055455	31.959798243136845	29.227377940027765	16.73140665628084
14	17.214166867712855	34.96564136227651	29.532344747146233	18.287847022864405
15	15.930027198899827	22.444983476808623	43.82856775942283	17.796421564868723
16	17.249014873677538	20.641552413208828	41.61219717612706	20.497235536986576
17	17.636644392619203	21.80550074839054	30.589442488010476	29.96841237097978
18	21.47578499964778	23.324474833069324	33.2584631094271	21.941277057855793
19	25.981837892562165	27.56146870318663	26.07640752592249	20.380285878328717
20	27.87503841696543	24.4710926751237	25.55973440707583	22.094134500835043
21	22.206782706162738	29.587577901501703	27.32376665176743	20.881872740568127
22	22.042267701259703	25.132893088935976	26.126466468301096	26.698372741503228
23	20.130489885662385	29.869790643902626	25.70760465778822	24.292114812646773
24	20.491375586073016	24.99013471029712	33.83504487537942	20.68344482825045
25	19.787682757219116	26.057643215018434	31.576844216945354	22.577829810817097
26	18.112796574297633	30.652966849509607	28.51103011079671	22.723206465396053
27	20.613437116804405	28.354930992844	28.728409353728455	22.303222536623135
28	19.205178700449906	27.7785362466017	34.278343927999906	18.73794112494849
29	19.716303567899686	24.511862971905277	33.04407618185547	22.727757278339563
30	21.297929255430898	27.96929635080908	30.194269840765187	20.53850455299484
31	26.75547609295877	26.187684253376798	23.832046326028966	23.224793327635464
32	27.06044290007724	27.19684260857585	25.636163128565453	20.10655136278146
33	25.184822228414657	27.959010266758682	24.92891692522142	21.92725057960525
34	19.38596442012357	28.830272755779063	28.93949226642329	22.844270557674072
35	21.768907225132455	26.441033620533265	28.401935279959154	23.38812387437512
36	26.100969447836775	25.91918628970974	27.17265472608158	20.807189536371908
37	21.961163487019896	28.49170474076263	27.837322775447305	21.709808996770168
38	20.739488401349284	29.040108870407185	25.977037720005313	24.243365008238218
39	20.824956408822594	27.22115517087679	27.462036557366115	24.491851862934503
40	23.219993155078615	24.030723597959987	28.180192243793904	24.56909100316749
41	18.85501546341302	23.08671044174679	28.968605001281084	29.089669093559106
42	22.774013486614688	25.884213603938385	25.410991397716735	25.93078151173019
43	22.026371025909086	25.768697763057247	27.512095499744717	24.692835711288946
44	22.21039842055621	28.400626141989104	27.751979447780666	21.63699598967402
45	19.93125155460134	29.886809437513286	26.309247064881504	23.872691943003872
46	23.27510162962741	28.333174366579826	26.798303606550427	21.593420397242333
47	22.4909903254704	25.296847034708986	27.551930697976264	24.66023194184435
48	23.11370361989117	23.41524173232617	28.49139304124595	24.979661606536713
49	22.086653712434753	22.577954490623767	29.26584166038592	26.06955013655556
50	20.66237394092297	26.331315390662358	29.012429953326112	23.993880715088565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2549.0
1	2234.5
2	1920.0
3	10754.0
4	19588.0
5	13757.0
6	7926.0
7	8147.5
8	8369.0
9	8992.5
10	9616.0
11	9860.5
12	10105.0
13	9849.0
14	9593.0
15	9151.5
16	8710.0
17	8357.5
18	8005.0
19	7656.5
20	7308.0
21	7037.0
22	6766.0
23	6956.0
24	7146.0
25	7879.0
26	8612.0
27	9493.0
28	10374.0
29	11949.0
30	13524.0
31	16612.5
32	19701.0
33	23141.0
34	26581.0
35	29370.0
36	32159.0
37	34645.0
38	37131.0
39	48996.0
40	60861.0
41	84330.5
42	107800.0
43	130542.5
44	153285.0
45	167783.5
46	182282.0
47	173234.0
48	164186.0
49	147443.0
50	130700.0
51	115829.5
52	100959.0
53	92015.5
54	83072.0
55	77530.0
56	71988.0
57	67306.5
58	62625.0
59	60571.5
60	58518.0
61	53773.5
62	49029.0
63	43563.5
64	38098.0
65	32393.5
66	26689.0
67	21789.0
68	16889.0
69	15570.5
70	14252.0
71	12774.0
72	11296.0
73	8978.5
74	6661.0
75	5333.0
76	4005.0
77	3366.5
78	2728.0
79	2044.5
80	1361.0
81	1035.5
82	710.0
83	483.5
84	257.0
85	194.0
86	131.0
87	76.0
88	21.0
89	15.0
90	9.0
91	9.0
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1604109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.318019248211538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49717591531966	21.170693433367383
2	7.711124093739068	4.213052728362232
3	2.85997551469767	2.34386598479774
4	1.6321841338947491	1.7835215034544893
5	1.1236811091115855	1.534837108378099
6	0.8290548867793068	1.3588882412916568
7	0.6532261982383081	1.2491392099837102
8	0.5272262805880644	1.1522222145014176
9	0.46387581397431055	1.140495157343701
>10	5.389952387119503	34.00550577277588
>50	1.1339895106154587	21.37482674515433
>100	0.1734856325457785	6.611614313348359
>500	0.003442175029518909	0.6871334253922968
>1k	0.001376870011807564	0.5711588941473603
>5k	0.0	0.0
>10k+	2.294783353012606E-4	0.8030452677014253
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12810	0.7985741617309049	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2253	0.1404518022154355	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2170	0.13527759023856858	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07954571665641175	0.0
2	0.0	0.0	0.0	0.31057739841868603	0.0
3	0.0	0.0	0.0	0.5535783416214235	0.0
4	0.0	0.0	0.0	0.8612881044866652	0.0
5	0.0	0.0	0.0	1.4202900176982987	0.0
6	0.0	0.0	0.0	2.645580817762384	0.0
7	0.0	0.0	0.0	3.406564017781834	0.0
8	0.0	0.0	0.0	5.627236054407774	0.0
9	0.0	0.0	0.0	6.947158827735522	0.0
10	0.0	0.0	0.0	8.690057845196305	0.0
11	0.0	0.0	0.0	9.500725948174345	0.0
12	0.0	0.0	0.0	10.341130185043536	0.0
13	0.0	0.0	0.0	10.83093480555249	0.0
14	0.0	0.0	0.0	11.061654787798085	0.0
15	0.0	0.0	0.0	11.23745331520489	0.0
16	0.0	0.0	0.0	11.550087930433655	0.0
17	0.0	0.0	0.0	11.862036806725728	0.0
18	0.0	0.0	0.0	12.42558953288087	0.0
19	0.0	0.0	0.0	12.645337692139375	0.0
20	0.0	0.0	0.0	12.916453931746533	0.0
21	0.0	0.0	0.0	13.173543693103149	0.0
22	0.0	0.0	0.0	13.425334562676227	0.0
23	0.0	0.0	0.0	13.721386763617684	0.0
24	0.0	0.0	0.0	13.954101622770024	0.0
25	0.0	0.0	0.0	14.145048746687413	0.0
26	0.0	0.0	0.0	14.333938653794723	0.0
27	0.0	0.0	0.0	14.5546842515066	0.0
28	0.0	0.0	0.0	14.763148888261334	0.0
29	0.0	0.0	0.0	14.968122490429266	0.0
30	0.0	0.0	0.0	15.262990233207345	0.0
31	0.0	0.0	0.0	15.485294328502615	0.0
32	0.0	0.0	0.0	15.700366995010938	0.0
33	0.0	0.0	0.0	15.92366852875958	0.0
34	0.0	0.0	0.0	16.13556186019778	0.0
35	0.0	0.0	0.0	16.375757507750407	0.0
36	0.0	0.0	0.0	16.594757588168882	0.0
37	0.0	0.0	0.0	16.825913949737828	0.0
38	0.0	0.0	0.0	17.04809336522643	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATC	30	2.5283462E-6	44.000004	20
CGAACCG	20	7.857414E-4	44.0	34
GACCGTT	25	4.4436307E-5	44.0	10
CAATCGA	20	7.857414E-4	44.0	28
TACGTAG	115	0.0	42.086952	1
CGTTTTT	8160	0.0	42.03186	1
CGGTCTA	545	0.0	41.98165	31
CACGACG	540	0.0	41.555553	26
TCGATAG	70	0.0	40.857143	1
TCACGAC	565	0.0	40.495575	25
CGACGGT	560	0.0	40.464287	28
CAACCCG	165	0.0	40.0	23
CTCACGT	105	0.0	39.809525	44
ACGTAAG	105	0.0	39.809525	1
GCGTAAG	135	0.0	39.11111	1
CTCACGA	595	0.0	38.45378	24
TAGCATA	665	0.0	38.37594	30
TACGGGA	645	0.0	38.20155	4
GGTACCT	730	0.0	37.972603	8
TCTCACG	605	0.0	37.81818	23
>>END_MODULE