FastQCFastQC Report
Thu 26 May 2016
SRR1547243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547243_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2662329
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160920.604433186131391No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA58290.218943639197109No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC207.858351E-444.00000434
AATGCGT207.858351E-444.00000420
TACGCTA351.4470425E-744.01
AGTCGTA302.5289773E-644.010
CGTTTTT94150.041.17261
TACGTAG1550.041.1612931
CTATGCG700.040.8571431
CGGTCTA5100.040.5490231
GCGATAC1700.040.117659
CGCGAAC557.8216544E-1140.00000444
TAATGCG950.039.3684231
AATACGG2350.039.3191452
GGCGATA12600.039.2857178
TTAGTAG5000.039.161
CACGACG5350.039.0654226
TACGTTC404.1272688E-738.50000431
ACATACG5450.037.94495417
CGCATCG1800.037.8888921
TAACGTA357.293822E-637.71428727
TTGCGAC357.293822E-637.71428743
Produced by FastQC (version 0.11.5)