FastQCFastQC Report
Thu 26 May 2016
SRR1547242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547242_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3744325
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505301.3495089235042363No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT47780.1276064444192211No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC43180.11532118606157318TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG43080.11505411522771128TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC41780.11158219438750641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG650.044.0000041
CGGTCGA207.858763E-444.024
CGTTTTT441600.043.3473741
CGACGGT5050.042.6930728
GCTACGA1150.040.1739110
GACGGTC5450.039.5596329
GTTTTTT494100.038.9463652
ACGGTCT5550.038.84684830
CGTTAGG7000.038.6571462
CTAAGCG404.127869E-738.51
CGCGTAA800.038.531
CGTAAGG5550.038.450452
TCGATAG1950.038.358971
AGGGCGA38750.038.038716
TCACGAC5700.037.8245625
TATCCGG2100.037.7142872
TACGGGA13750.037.64
GGCACCG12450.036.9317258
CGGTCTA5850.036.85470231
TGCGGGT8500.036.752944
Produced by FastQC (version 0.11.5)