##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547242_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3744325
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246572880292174	31.0	31.0	33.0	30.0	34.0
2	31.63432794963044	31.0	31.0	34.0	30.0	34.0
3	31.699037343179345	31.0	31.0	34.0	30.0	34.0
4	35.42656446756091	37.0	35.0	37.0	33.0	37.0
5	35.314288156076195	37.0	35.0	37.0	33.0	37.0
6	35.349137427974334	37.0	35.0	37.0	33.0	37.0
7	35.93663103496625	37.0	35.0	37.0	35.0	37.0
8	35.927580538548334	37.0	35.0	37.0	35.0	37.0
9	37.61867172320779	39.0	37.0	39.0	35.0	39.0
10	36.90179217883063	39.0	37.0	39.0	32.0	39.0
11	36.707571858746235	39.0	35.0	39.0	32.0	39.0
12	36.50363309808844	39.0	35.0	39.0	32.0	39.0
13	36.435587989824604	39.0	35.0	39.0	32.0	39.0
14	37.54309975763322	40.0	36.0	41.0	32.0	41.0
15	37.667845873421776	40.0	36.0	41.0	32.0	41.0
16	37.68457145146321	40.0	36.0	41.0	32.0	41.0
17	37.620227677885865	40.0	36.0	41.0	32.0	41.0
18	37.438947206772916	39.0	36.0	41.0	32.0	41.0
19	37.29239742810787	38.0	36.0	41.0	32.0	41.0
20	37.00060678493453	38.0	35.0	41.0	32.0	41.0
21	36.90230174998164	38.0	35.0	41.0	32.0	41.0
22	36.83281285678994	38.0	35.0	40.0	31.0	41.0
23	36.70942693275824	38.0	35.0	40.0	31.0	41.0
24	36.6670155501993	38.0	35.0	40.0	31.0	41.0
25	36.4921776288116	38.0	35.0	40.0	31.0	41.0
26	36.40387626608267	38.0	35.0	40.0	31.0	41.0
27	36.27129776394944	38.0	35.0	40.0	30.0	41.0
28	36.312069598659306	38.0	35.0	40.0	31.0	41.0
29	36.21358268846855	38.0	35.0	40.0	30.0	41.0
30	36.12927670541419	38.0	35.0	40.0	30.0	41.0
31	35.89269174016678	38.0	35.0	40.0	30.0	41.0
32	35.43121871098262	38.0	34.0	40.0	26.0	41.0
33	35.09839076469056	38.0	34.0	40.0	24.0	41.0
34	34.7718376476401	38.0	34.0	40.0	22.0	41.0
35	34.42437956106908	38.0	34.0	40.0	19.0	41.0
36	34.247528726806564	38.0	34.0	40.0	18.0	41.0
37	34.19337584210772	38.0	33.0	40.0	18.0	41.0
38	34.14700246372844	38.0	33.0	40.0	18.0	41.0
39	34.01599487223999	38.0	33.0	40.0	17.0	41.0
40	33.95387927062955	38.0	33.0	40.0	16.0	41.0
41	33.94357968392167	38.0	33.0	40.0	15.0	41.0
42	33.73446909656614	38.0	33.0	40.0	15.0	41.0
43	33.75089608941531	38.0	33.0	40.0	15.0	41.0
44	33.49360111635608	38.0	33.0	40.0	15.0	41.0
45	33.408333144158156	37.0	33.0	40.0	15.0	41.0
46	33.40220734044187	37.0	33.0	40.0	14.0	41.0
47	33.30361867626341	37.0	33.0	40.0	13.0	41.0
48	33.2468546400219	37.0	33.0	40.0	13.0	41.0
49	33.23228058461805	37.0	32.0	40.0	12.0	41.0
50	32.671915231717335	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	5.0
13	17.0
14	28.0
15	121.0
16	307.0
17	932.0
18	2197.0
19	4562.0
20	7901.0
21	12745.0
22	19446.0
23	28062.0
24	41843.0
25	64805.0
26	95140.0
27	107488.0
28	101259.0
29	93814.0
30	98595.0
31	112559.0
32	135977.0
33	167805.0
34	239386.0
35	344725.0
36	278903.0
37	339546.0
38	505276.0
39	940874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.311558158012456	23.36151375748633	27.483431593144292	19.84349649135692
2	36.443738190461566	24.328897731900945	26.808623717225398	12.418740360412091
3	25.076428995880434	23.837433983428255	38.59360498888318	12.492532031808135
4	21.814799730258457	26.431973720229944	38.181621520567795	13.5716050289438
5	20.349996327776037	30.17147282888104	36.97852082818665	12.50001001515627
6	17.056505511674334	38.71741902746156	33.86970949370047	10.356365967163642
7	79.19443424382231	3.244216247254178	15.340201504944147	2.2211480039793554
8	78.36274896009294	2.9034605703297656	14.694397521582662	4.039392947994632
9	71.89175619103577	5.655785755777076	18.27982346617882	4.172634587008339
10	31.51366401153746	30.71691159287722	24.388775012852783	13.380649382732535
11	25.630200369893103	25.241051457872913	34.65612627109025	14.47262190114373
12	23.125182776601925	21.55998744767081	37.180586621086576	18.13424315464069
13	19.431112416790743	26.882442095704835	39.06084541272459	14.625600074779834
14	16.290225875157738	30.520000267070834	36.028843650057084	17.160930207714344
15	15.303425851121363	24.543515853992375	45.03097353995714	15.122084754929126
16	16.134283215265768	24.254838989671036	42.21033163520795	17.40054615985525
17	16.968639207333766	25.031454267458088	36.20978413999853	21.790122385209617
18	19.09858252154928	24.260260527598433	38.82536905850854	17.815787892343746
19	21.090717285492044	28.20265334873442	33.88616105706636	16.820468308707177
20	21.769370981418547	25.629212207807818	33.565569228098525	19.035847582675114
21	19.661060404745847	29.027207841199683	34.113144558765605	17.19858719528887
22	18.69434410741589	25.214985344487996	33.48272385543456	22.607946692661564
23	16.55548062734939	29.465844978734484	33.23549638452859	20.743178009387538
24	16.93747204102208	25.74592216220547	38.05967697782645	19.256928818946005
25	16.820949036208127	27.625913883009623	36.15351231530383	19.399624765478425
26	16.77194153819447	29.095337610917856	35.44451937265061	18.688201478237065
27	19.01974321139324	27.484232805645874	33.70089401961635	19.79512996334453
28	17.843509844898612	27.29469263485408	37.49565008379348	17.366147436453833
29	18.10499889833281	25.398623249851443	35.30556241779226	21.190815434023488
30	19.591728816275296	27.833374506753554	33.128561222650276	19.446335454320874
31	21.71779960339981	25.903173469183365	30.881427226536157	21.497599700880667
32	21.540758347632753	27.42467600969467	30.44353788733617	20.59102775533641
33	21.431766740333703	27.253109706021778	29.565702763515457	21.74942079012906
34	18.48026012699218	28.197258517890404	30.172193920132468	23.150287434984946
35	18.998030352600267	26.503815774538804	29.811888658169362	24.686265214691566
36	20.690565055116743	27.988729610811024	29.57387513103163	21.746830203040602
37	18.79473603386458	28.214724950424973	30.46848230321887	22.52205671249157
38	18.10232818999419	29.31324070426579	31.21294225260895	21.37148885313107
39	18.029230902766187	27.309434944883254	31.790536345002103	22.870797807348453
40	20.183691319530222	26.176974488058598	32.63394603833802	21.005388154073167
41	17.578655698957757	25.543669419721848	32.0833528072483	24.794322074072095
42	20.517155962690204	26.884685490709277	30.438784026493426	22.159374520107093
43	20.270836532619363	25.954905089702418	31.86237839931096	21.911879978367264
44	20.69836352346551	28.6833007284357	29.936130010081925	20.682205738016865
45	18.411035366855174	28.75789361233333	30.470218263638976	22.360852757172523
46	21.62314969987915	28.66017239422326	28.741388634800664	20.975289271096926
47	20.332556602324853	25.75438830763889	30.582815327195156	23.3302397628411
48	20.862665500457357	24.823913522463993	30.942800104157627	23.370620872921023
49	20.849632443764897	23.654009734731897	30.829214878516154	24.667142942987052
50	19.503114713599913	25.74872640596102	31.88499929893906	22.863159581500003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4181.0
1	3996.0
2	3811.0
3	41975.5
4	80140.0
5	59229.5
6	38319.0
7	39642.0
8	40965.0
9	42907.0
10	44849.0
11	45708.0
12	46567.0
13	45877.0
14	45187.0
15	43491.5
16	41796.0
17	39684.0
18	37572.0
19	36555.5
20	35539.0
21	34066.0
22	32593.0
23	32373.0
24	32153.0
25	33437.0
26	34721.0
27	38567.0
28	42413.0
29	48730.0
30	55047.0
31	61411.5
32	67776.0
33	79244.5
34	90713.0
35	101098.0
36	111483.0
37	123658.0
38	135833.0
39	158083.0
40	180333.0
41	207353.0
42	234373.0
43	267430.5
44	300488.0
45	333755.5
46	367023.0
47	390813.0
48	414603.0
49	397541.5
50	380480.0
51	320105.5
52	259731.0
53	222805.0
54	185879.0
55	158259.5
56	130640.0
57	111265.5
58	91891.0
59	78726.0
60	65561.0
61	55301.0
62	45041.0
63	36047.5
64	27054.0
65	21562.5
66	16071.0
67	12360.5
68	8650.0
69	7360.5
70	6071.0
71	4939.0
72	3807.0
73	3118.5
74	2430.0
75	1758.5
76	1087.0
77	929.0
78	771.0
79	558.5
80	346.0
81	271.0
82	196.0
83	131.5
84	67.0
85	54.0
86	41.0
87	24.5
88	8.0
89	8.0
90	8.0
91	8.5
92	9.0
93	7.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3744325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.435886217037066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34308063664402	23.84441088149057
2	8.113792669446026	4.939009409517774
3	3.1325509473079465	2.86025892604009
4	1.7107780095974794	2.082761793708721
5	1.1308583883214958	1.7209338617267484
6	0.8163128291926232	1.4907122632088576
7	0.6151629391689165	1.3106120455038268
8	0.5021119927483888	1.222577878359976
9	0.41890962860889663	1.147489721240547
>10	4.188746301011717	27.729251789297372
>50	0.7465213205047786	15.913115440665662
>100	0.2750456289648951	11.910512641394675
>500	0.003552874482722714	0.7192051101496252
>1k	0.002487012137805369	1.743134321942493
>5k	0.0	0.0
>10k+	8.882186206447747E-5	1.3660139157530116
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50530	1.3495089235042363	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	4778	0.1276064444192211	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	4318	0.11532118606157318	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	4308	0.11505411522771128	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	4178	0.11158219438750641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6707083386191103E-5	0.0	0.0	0.12090296648928713	0.0
2	2.6707083386191103E-5	0.0	0.0	0.4325746296061373	0.0
3	2.6707083386191103E-5	0.0	0.0	0.761926381924646	0.0
4	2.6707083386191103E-5	0.0	0.0	1.1725744960707203	0.0
5	2.6707083386191103E-5	0.0	0.0	1.9132420396199583	0.0
6	2.6707083386191103E-5	0.0	0.0	3.3618342424869634	0.0
7	2.6707083386191103E-5	0.0	0.0	4.332396359824535	0.0
8	2.6707083386191103E-5	0.0	0.0	6.981899274235009	0.0
9	2.6707083386191103E-5	0.0	0.0	8.54853678566898	0.0
10	2.6707083386191103E-5	0.0	0.0	10.67183003612133	0.0
11	2.6707083386191103E-5	0.0	0.0	11.646585165550533	0.0
12	2.6707083386191103E-5	0.0	0.0	12.712411449326648	0.0
13	2.6707083386191103E-5	0.0	0.0	13.340962656820656	0.0
14	2.6707083386191103E-5	0.0	0.0	13.642378799917209	0.0
15	2.6707083386191103E-5	0.0	0.0	13.889686392073338	0.0
16	2.6707083386191103E-5	0.0	0.0	14.319510192090696	0.0
17	2.6707083386191103E-5	0.0	0.0	14.762046563799883	0.0
18	2.6707083386191103E-5	0.0	0.0	15.543121924512429	0.0
19	2.6707083386191103E-5	0.0	0.0	15.83417571925514	0.0
20	2.6707083386191103E-5	0.0	0.0	16.21021145333271	0.0
21	2.6707083386191103E-5	0.0	0.0	16.54439718774412	0.0
22	2.6707083386191103E-5	0.0	0.0	16.88755650217329	0.0
23	2.6707083386191103E-5	0.0	0.0	17.28426351879177	0.0
24	2.6707083386191103E-5	0.0	0.0	17.592009240650853	0.0
25	5.341416677238221E-5	0.0	0.0	17.861029691599956	0.0
26	5.341416677238221E-5	0.0	0.0	18.120408885446643	0.0
27	5.341416677238221E-5	0.0	0.0	18.427139738137047	0.0
28	5.341416677238221E-5	0.0	0.0	18.70711009327449	0.0
29	8.012125015857331E-5	0.0	0.0	19.01514959305082	0.0
30	8.012125015857331E-5	0.0	0.0	19.427907566784402	0.0
31	8.012125015857331E-5	0.0	0.0	19.74233005949003	0.0
32	8.012125015857331E-5	0.0	0.0	20.055123420109098	0.0
33	8.012125015857331E-5	0.0	0.0	20.359557463628292	0.0
34	8.012125015857331E-5	0.0	0.0	20.665086497566318	0.0
35	8.012125015857331E-5	0.0	0.0	20.983702002363575	0.0
36	8.012125015857331E-5	0.0	0.0	21.290459562137368	0.0
37	8.012125015857331E-5	0.0	0.0	21.6035199935903	0.0
38	8.012125015857331E-5	0.0	0.0	21.928465077150086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	65	0.0	44.000004	1
CGGTCGA	20	7.858763E-4	44.0	24
CGTTTTT	44160	0.0	43.347374	1
CGACGGT	505	0.0	42.69307	28
GCTACGA	115	0.0	40.17391	10
GACGGTC	545	0.0	39.55963	29
GTTTTTT	49410	0.0	38.946365	2
ACGGTCT	555	0.0	38.846848	30
CGTTAGG	700	0.0	38.657146	2
CTAAGCG	40	4.127869E-7	38.5	1
CGCGTAA	80	0.0	38.5	31
CGTAAGG	555	0.0	38.45045	2
TCGATAG	195	0.0	38.35897	1
AGGGCGA	3875	0.0	38.03871	6
TCACGAC	570	0.0	37.82456	25
TATCCGG	210	0.0	37.714287	2
TACGGGA	1375	0.0	37.6	4
GGCACCG	1245	0.0	36.931725	8
CGGTCTA	585	0.0	36.854702	31
TGCGGGT	850	0.0	36.75294	4
>>END_MODULE