FastQCFastQC Report
Thu 26 May 2016
SRR1547241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547241_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2903708
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273910.9433111042845906No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC32420.11165034500714259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT302.5290574E-644.019
TACCGTA254.4445238E-544.044
CGTTTTT176800.042.593891
TCGTAAG2050.041.853661
CGACGGT5750.040.9391328
CGGTCTA6100.038.9508231
TAGCCGT2950.038.77966344
ACGGTCT6100.038.22950730
CGTTAGG5500.038.02
AGGGCGA27050.037.818856
GTCGACA357.2940475E-637.7142879
CTATACG700.037.7142871
AGGGTAC21250.037.6847046
TCACGAC6600.037.66666825
CTCACGA6850.037.5766424
CACGACG6450.037.51938226
GGGCGAT59650.037.4350367
GTTTTTT205600.037.3871572
TAGGGTA23850.037.1740045
GCGTAAG2900.037.1724171
Produced by FastQC (version 0.11.5)