##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547241_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2903708
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409910018500483	31.0	31.0	33.0	30.0	34.0
2	31.80849623998005	31.0	31.0	34.0	30.0	34.0
3	31.932140559587946	33.0	31.0	34.0	30.0	34.0
4	35.625426523603615	37.0	35.0	37.0	33.0	37.0
5	35.524015155793904	37.0	35.0	37.0	33.0	37.0
6	35.56231308382248	37.0	35.0	37.0	33.0	37.0
7	36.03472973177744	37.0	35.0	37.0	35.0	37.0
8	36.03247881674053	37.0	35.0	37.0	35.0	37.0
9	37.75711469610581	39.0	38.0	39.0	35.0	39.0
10	36.988544647051285	39.0	37.0	39.0	32.0	39.0
11	36.88799321419371	39.0	37.0	39.0	32.0	39.0
12	36.5723244210506	39.0	35.0	39.0	32.0	39.0
13	36.46166969957034	39.0	35.0	39.0	32.0	39.0
14	37.55602457271875	40.0	36.0	41.0	32.0	41.0
15	37.70396059107872	40.0	36.0	41.0	33.0	41.0
16	37.73234188837169	40.0	36.0	41.0	33.0	41.0
17	37.645618292197426	40.0	36.0	41.0	33.0	41.0
18	37.57823720566944	39.0	36.0	41.0	33.0	41.0
19	37.51732095651491	39.0	36.0	41.0	33.0	41.0
20	37.345731388968865	39.0	35.0	41.0	32.0	41.0
21	37.26405031084393	39.0	35.0	41.0	32.0	41.0
22	37.19939091671752	39.0	35.0	41.0	32.0	41.0
23	37.1092124276959	39.0	35.0	41.0	32.0	41.0
24	37.06250559629274	39.0	35.0	41.0	32.0	41.0
25	36.8904896773367	38.0	35.0	41.0	31.0	41.0
26	36.81261511143683	38.0	35.0	41.0	31.0	41.0
27	36.7288980159162	38.0	35.0	41.0	31.0	41.0
28	36.779028400927366	38.0	35.0	41.0	31.0	41.0
29	36.70062726692905	38.0	35.0	41.0	31.0	41.0
30	36.64415051375689	38.0	35.0	41.0	31.0	41.0
31	36.51725001274233	38.0	35.0	40.0	31.0	41.0
32	36.27677232008177	38.0	35.0	41.0	30.0	41.0
33	36.144076126111855	38.0	35.0	41.0	30.0	41.0
34	35.97120819311033	38.0	35.0	41.0	29.0	41.0
35	35.757066826278674	38.0	35.0	41.0	28.0	41.0
36	35.59030970056218	38.0	35.0	41.0	27.0	41.0
37	35.541231418586165	38.0	35.0	41.0	27.0	41.0
38	35.44970189840025	38.0	35.0	40.0	27.0	41.0
39	35.36110586877193	38.0	35.0	40.0	26.0	41.0
40	35.21662474325931	38.0	34.0	40.0	25.0	41.0
41	35.19300184453809	38.0	34.0	40.0	25.0	41.0
42	35.00758271837251	38.0	34.0	40.0	25.0	41.0
43	35.04641582418067	38.0	34.0	40.0	25.0	41.0
44	34.79963550053931	38.0	34.0	40.0	24.0	41.0
45	34.713723280715556	37.0	34.0	40.0	24.0	41.0
46	34.704900079484574	37.0	34.0	40.0	24.0	41.0
47	34.61168030669751	37.0	34.0	40.0	24.0	41.0
48	34.568630523454836	37.0	34.0	40.0	23.0	41.0
49	34.559315881624464	37.0	34.0	40.0	24.0	41.0
50	34.03554283006418	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	4.0
13	14.0
14	20.0
15	62.0
16	157.0
17	472.0
18	1033.0
19	1977.0
20	3674.0
21	6060.0
22	9250.0
23	13970.0
24	20457.0
25	30284.0
26	43973.0
27	53258.0
28	56477.0
29	59266.0
30	67433.0
31	80604.0
32	99886.0
33	127913.0
34	200402.0
35	305927.0
36	222418.0
37	282566.0
38	428705.0
39	787439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.76896299490169	23.38861207807397	27.7205214849427	16.121903442081642
2	34.51431755534647	24.244138873467993	28.09521480810054	13.146328763084995
3	28.33408180161366	24.7498026661083	33.51425143299533	13.40186409928271
4	24.87943002533313	27.54013144572388	33.04106335761034	14.539375171332654
5	22.163867716726337	31.569221147580954	31.910887733890597	14.356023401802108
6	18.937028103376786	40.31903345653213	29.490740804516157	11.253197635574926
7	85.8002939689528	3.251359985232675	8.995945873345391	1.9524001724691327
8	85.66074825705616	2.6128660319839323	8.64725378722654	3.079131923733378
9	80.1943583858983	4.528933350047595	11.91562650238936	3.36108176166474
10	34.96973524886111	26.284977690594236	24.840032124442267	13.90525493610239
11	32.066585207603524	24.455317132438935	27.114641003847495	16.36345665611005
12	29.472419403052925	21.722259951758236	29.27312250405344	19.532198141135403
13	22.80694202034089	28.09752220264572	30.685282404429092	18.410253372584297
14	19.276456172590358	31.13388123048185	30.72247622694844	18.86718636997935
15	18.556308003421833	23.725663875293247	38.99424460035238	18.723783520932546
16	19.917774101252604	21.601311151121255	38.29345099438373	20.187463753242408
17	19.889706540740324	22.339367457058355	31.74723491480548	26.02369108739584
18	22.390818911543448	23.562183249830905	33.06145108254687	20.98554675607878
19	25.08923073532187	27.339043733047536	27.811164207971323	19.760561323659264
20	26.5460576614453	25.170850512517102	27.22491379987244	21.058178026165166
21	23.17009837077282	28.309389236107762	29.13443776027066	19.386074632848757
22	22.02811026453073	23.29810710994356	29.61637327169261	25.057409353833098
23	19.917808539977162	28.37234322459421	28.302983633340546	23.40686460208809
24	20.541080576972615	25.80410977963349	33.42874007992539	20.226069563468503
25	21.281134328933902	26.192509715164196	30.892018067932455	21.63433788796945
26	19.02694761318976	28.56213503561653	29.513332607824204	22.89758474336951
27	20.52482549898268	26.48503224153393	29.94181921873687	23.04832304074652
28	19.58251311771018	26.70998599032685	33.78359669773958	19.923904194223386
29	20.65913652474698	24.584978930388317	32.3684750670522	22.387409477812508
30	21.89531454264685	25.243895047298142	32.294259615636285	20.56653079441872
31	25.723488725450355	24.851121393748958	27.623955301290625	21.80143457951006
32	25.247752184448295	25.25970242186887	28.936621726427035	20.555923667255797
33	24.641389561209323	25.525156110738408	27.054752061846436	22.77870226620583
34	20.67577042870702	27.027717663070806	30.385389991004608	21.91112191721757
35	21.230337210215353	26.18138600713295	29.474795675047215	23.113481107604482
36	24.328548187352173	27.791844083496	27.081752021897522	20.79785570725431
37	22.08593288305849	29.443215364630326	27.133513424903605	21.337338327407576
38	21.536084206814184	30.96154985280889	26.301818226901602	21.20054771347532
39	22.451534382933822	28.66042315549635	25.647895725052244	23.240146736517584
40	23.364918235580163	26.38016632526411	28.55218224422015	21.70273319493558
41	21.25871471924863	25.2383504126448	28.579251081720336	24.923683786386235
42	23.2360829670201	27.07903136265768	26.200499499260943	23.484386171061278
43	22.560532946150232	25.629023303996135	28.190541197668633	23.619902552185
44	22.693500861656887	27.128623126016805	28.682946081355286	21.494929930971022
45	21.02687322554472	27.864165405061392	27.95639230941954	23.15256905997435
46	23.26087884869966	27.212756930104543	27.85052767013763	21.675836551058165
47	22.396156913849463	26.571232369094965	27.914790330157164	23.11782038689841
48	22.659234330724715	25.691564027787916	28.418215605701402	23.230986035785968
49	22.925376794085356	24.21007897488315	28.462779315275505	24.40176491575599
50	20.964435817926596	25.53280150758961	30.007424989014048	23.49533768546975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3104.0
1	3217.5
2	3331.0
3	23754.5
4	44178.0
5	30509.5
6	16841.0
7	17126.5
8	17412.0
9	18219.5
10	19027.0
11	19028.5
12	19030.0
13	18483.0
14	17936.0
15	16847.0
16	15758.0
17	15055.5
18	14353.0
19	13690.0
20	13027.0
21	12942.5
22	12858.0
23	13233.0
24	13608.0
25	14861.5
26	16115.0
27	20064.0
28	24013.0
29	27545.0
30	31077.0
31	36924.0
32	42771.0
33	49893.0
34	57015.0
35	64995.5
36	72976.0
37	79024.0
38	85072.0
39	101835.5
40	118599.0
41	147033.0
42	175467.0
43	205148.0
44	234829.0
45	255378.0
46	275927.0
47	277461.5
48	278996.0
49	262581.0
50	246166.0
51	222110.0
52	198054.0
53	182644.0
54	167234.0
55	155409.0
56	143584.0
57	138743.0
58	133902.0
59	120844.0
60	107786.0
61	96307.5
62	84829.0
63	75364.5
64	65900.0
65	54195.5
66	42491.0
67	34980.0
68	27469.0
69	25676.5
70	23884.0
71	20355.5
72	16827.0
73	13803.0
74	10779.0
75	8063.0
76	5347.0
77	4396.0
78	3445.0
79	2371.0
80	1297.0
81	1088.5
82	880.0
83	583.0
84	286.0
85	203.0
86	120.0
87	81.5
88	43.0
89	43.0
90	43.0
91	29.0
92	15.0
93	9.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2903708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.916889152185828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73052996194737	18.590724688405547
2	7.3102137608869455	3.496751443958331
3	2.7778885946702356	1.9931536078754788
4	1.5471990133448978	1.480167491941648
5	1.0315827014396806	1.2336124560822623
6	0.7571198966006683	1.0864771585147548
7	0.6320116632761827	1.058102702542554
8	0.5422684167645304	1.0375498891590864
9	0.43672188878213036	0.9400526103901895
>10	5.428694735329227	30.601935985256656
>50	1.4247830407236843	23.78211497133808
>100	0.37301474593849826	11.84028858152629
>500	0.00521776164831013	0.8507044050977739
>1k	0.002608880824155065	1.0588654397993809
>5k	0.0	0.0
>10k+	1.449378235641703E-4	0.9494985681118587
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27391	0.9433111042845906	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	3242	0.11165034500714259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.066019034971836	0.0
2	0.0	0.0	0.0	0.2412432655074133	0.0
3	0.0	0.0	0.0	0.4577250880598187	0.0
4	0.0	0.0	0.0	0.6738969620912295	0.0
5	0.0	0.0	0.0	1.0382931066071381	0.0
6	0.0	0.0	0.0	1.9680009146925241	0.0
7	0.0	0.0	0.0	2.53754854138226	0.0
8	0.0	0.0	0.0	4.16818771033451	0.0
9	0.0	0.0	0.0	5.110396775433342	0.0
10	0.0	0.0	0.0	6.410424188658088	0.0
11	0.0	0.0	0.0	7.04306355873249	0.0
12	0.0	0.0	0.0	7.650218272636229	0.0
13	0.0	0.0	0.0	8.010412892756435	0.0
14	0.0	0.0	0.0	8.184431767932589	0.0
15	0.0	0.0	0.0	8.320671362271964	0.0
16	0.0	0.0	0.0	8.555922289706816	0.0
17	0.0	0.0	0.0	8.788728067698267	0.0
18	0.0	0.0	0.0	9.194416242955558	0.0
19	0.0	0.0	0.0	9.341159648284194	0.0
20	0.0	0.0	0.0	9.53739150079829	0.0
21	0.0	0.0	0.0	9.724910355999983	0.0
22	0.0	0.0	0.0	9.918938130142562	0.0
23	0.0	0.0	0.0	10.14461509215114	0.0
24	0.0	0.0	0.0	10.322525543202003	0.0
25	0.0	0.0	0.0	10.468098031895769	0.0
26	0.0	0.0	0.0	10.619249593967437	0.0
27	0.0	0.0	0.0	10.820165113021007	0.0
28	0.0	0.0	0.0	10.984127880627115	0.0
29	0.0	0.0	0.0	11.153256456916466	0.0
30	0.0	0.0	0.0	11.396600484621732	0.0
31	0.0	0.0	0.0	11.571790276432754	0.0
32	0.0	0.0	0.0	11.746704558447338	0.0
33	0.0	0.0	0.0	11.928265514301025	0.0
34	0.0	0.0	0.0	12.096636438650167	0.0
35	0.0	0.0	0.0	12.287048146714477	0.0
36	0.0	0.0	0.0	12.457657588159691	0.0
37	0.0	0.0	0.0	12.6424902228461	0.0
38	0.0	0.0	0.0	12.8485026731338	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	30	2.5290574E-6	44.0	19
TACCGTA	25	4.4445238E-5	44.0	44
CGTTTTT	17680	0.0	42.59389	1
TCGTAAG	205	0.0	41.85366	1
CGACGGT	575	0.0	40.93913	28
CGGTCTA	610	0.0	38.95082	31
TAGCCGT	295	0.0	38.779663	44
ACGGTCT	610	0.0	38.229507	30
CGTTAGG	550	0.0	38.0	2
AGGGCGA	2705	0.0	37.81885	6
GTCGACA	35	7.2940475E-6	37.714287	9
CTATACG	70	0.0	37.714287	1
AGGGTAC	2125	0.0	37.684704	6
TCACGAC	660	0.0	37.666668	25
CTCACGA	685	0.0	37.57664	24
CACGACG	645	0.0	37.519382	26
GGGCGAT	5965	0.0	37.435036	7
GTTTTTT	20560	0.0	37.387157	2
TAGGGTA	2385	0.0	37.174004	5
GCGTAAG	290	0.0	37.172417	1
>>END_MODULE