FastQCFastQC Report
Thu 26 May 2016
SRR1547240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547240_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2722234
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238520.8761921274952851No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC32650.11993825659366536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151300.042.051551
TACGTAC650.040.61538721
CGGTCTA6900.038.8985531
ACGGTCT6900.038.8985530
CGACGGT7000.038.34285728
TAGGGTA16850.038.124635
TCACGAC7100.038.11267525
ACGGGCT9200.037.5434765
CGCATCG3000.037.421
ATGGTCG3000.037.444
CGTACGA651.0913936E-1137.2307723
AAGGGAT29000.037.0965545
CGCTGAA20550.036.93430737
TCGTTTA3100.036.90322538
TAGCATA22100.036.7330330
TAGGGCA16900.036.7100565
TAAGGGA27350.036.6800734
AGGGATT28850.036.679386
CTATCCG301.3015632E-436.66666828
GCGTAAG1200.036.6666681
Produced by FastQC (version 0.11.5)