##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547240_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2722234
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.328237763542738	31.0	31.0	33.0	30.0	34.0
2	31.728506072585972	31.0	31.0	34.0	30.0	34.0
3	31.855827970703473	31.0	31.0	34.0	30.0	34.0
4	35.544814295905496	37.0	35.0	37.0	33.0	37.0
5	35.432095110119114	37.0	35.0	37.0	33.0	37.0
6	35.46585965791332	37.0	35.0	37.0	33.0	37.0
7	35.957897814809456	37.0	35.0	37.0	35.0	37.0
8	35.958143201502885	37.0	35.0	37.0	35.0	37.0
9	37.66412182053416	39.0	37.0	39.0	35.0	39.0
10	36.99040971496205	39.0	37.0	39.0	32.0	39.0
11	36.76192274433425	39.0	37.0	39.0	32.0	39.0
12	36.38114137138835	39.0	35.0	39.0	32.0	39.0
13	36.26475314025172	39.0	35.0	39.0	32.0	39.0
14	37.30205742783317	39.0	35.0	41.0	32.0	41.0
15	37.45647251485361	39.0	35.0	41.0	32.0	41.0
16	37.50473397951829	39.0	35.0	41.0	32.0	41.0
17	37.41955504192512	39.0	35.0	41.0	32.0	41.0
18	37.33814616965331	39.0	36.0	41.0	32.0	41.0
19	37.29433840000529	39.0	35.0	41.0	32.0	41.0
20	37.11948899323129	39.0	35.0	41.0	32.0	41.0
21	37.01727955789252	39.0	35.0	41.0	32.0	41.0
22	36.964881417247746	39.0	35.0	41.0	31.0	41.0
23	36.875443477673116	38.0	35.0	41.0	31.0	41.0
24	36.81021800477108	38.0	35.0	41.0	31.0	41.0
25	36.61398689458731	38.0	35.0	41.0	31.0	41.0
26	36.510609668382656	38.0	35.0	41.0	31.0	41.0
27	36.419185125158236	38.0	35.0	40.0	30.0	41.0
28	36.46355456584555	38.0	35.0	40.0	31.0	41.0
29	36.36562103037432	38.0	35.0	40.0	30.0	41.0
30	36.31299660499428	38.0	35.0	40.0	30.0	41.0
31	36.16176456542678	38.0	35.0	40.0	30.0	41.0
32	35.95584508899676	38.0	35.0	40.0	30.0	41.0
33	35.79097498598578	38.0	35.0	40.0	29.0	41.0
34	35.64870911170752	38.0	35.0	40.0	28.0	41.0
35	35.42355359605383	38.0	35.0	40.0	27.0	41.0
36	35.29877005430099	38.0	34.0	40.0	26.0	41.0
37	35.25960148907111	38.0	34.0	40.0	26.0	41.0
38	35.21316022061292	38.0	34.0	40.0	26.0	41.0
39	35.110760500383144	38.0	34.0	40.0	25.0	41.0
40	34.98489182046804	38.0	34.0	40.0	25.0	41.0
41	34.9428877164858	38.0	34.0	40.0	24.0	41.0
42	34.751488299683274	37.0	34.0	40.0	24.0	41.0
43	34.74099544712174	37.0	34.0	40.0	24.0	41.0
44	34.37966280635684	37.0	33.0	40.0	23.0	41.0
45	34.314311333999946	37.0	33.0	40.0	23.0	41.0
46	34.31941743435722	36.0	33.0	40.0	23.0	41.0
47	34.21889374682706	36.0	33.0	40.0	23.0	41.0
48	34.169410491530115	36.0	33.0	40.0	23.0	41.0
49	34.147929604876	36.0	33.0	40.0	23.0	41.0
50	33.601400173533946	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	4.0
13	10.0
14	30.0
15	61.0
16	203.0
17	500.0
18	1163.0
19	2427.0
20	4481.0
21	7100.0
22	10779.0
23	15424.0
24	21621.0
25	31246.0
26	43101.0
27	52261.0
28	56694.0
29	61221.0
30	69148.0
31	82550.0
32	101413.0
33	127398.0
34	199323.0
35	292451.0
36	214189.0
37	268380.0
38	392559.0
39	666491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.903429315775206	24.00274921259524	28.03825828345396	16.055563188175594
2	33.529336566952	24.723113442856125	28.26288996463934	13.484660025552541
3	29.292007961108414	24.55648559234805	32.59356102377679	13.557945422766743
4	25.05486302793955	27.59171327666909	32.478765602075356	14.874658093316004
5	22.512576068038236	31.70715669556695	31.49872494429208	14.281542292102737
6	19.020150361798436	41.38461278494061	28.744259310551556	10.850977542709407
7	85.53489523677979	3.855546584165799	8.60488113806528	2.004677040989129
8	85.47042612795227	2.894828291763309	8.316661976891039	3.3180836033933896
9	80.18215921188259	4.848628001854359	11.193784222811118	3.77542856345193
10	39.2083487312259	27.2265352647862	17.53938125818721	16.02573474580069
11	32.72701758923002	24.29776426273421	26.928177371967287	16.04704077606848
12	30.479819148537562	20.76268976142389	29.0699109628342	19.68758012720435
13	24.15971588041293	25.92440620460989	30.818144215376048	19.097733699601136
14	19.95037899019702	29.908560395616245	30.512329211963408	19.62873140222332
15	18.424499877674	22.87073778374673	38.89044806581653	19.814314272762736
16	20.527882614058896	20.808314053824912	37.481311305347006	21.18249202676919
17	20.748473496400383	22.163855127810468	31.09791443351306	25.989756942276088
18	22.839072614624605	23.496841197340128	32.37297014143531	21.29111604659996
19	26.438616224762455	26.402616380516886	27.090066467467526	20.06870092725313
20	26.889422437600878	25.172964557785992	26.26317208586771	21.674440918745415
21	23.87050488679518	27.90579355044423	28.56811721549286	19.65558434726772
22	22.79348505675853	23.53063696948903	28.150886367593674	25.524991606158764
23	21.02027232045445	27.783687956288844	27.23006178014087	23.96597794311584
24	21.997300746372282	25.170723751154384	32.079681614438734	20.752293888034608
25	22.46691504110227	25.28919262635027	29.765001833053294	22.478890499494163
26	19.640192577125994	27.795149131191515	28.608451734861884	23.95620655682061
27	21.055243597721578	26.177103070492834	29.43703590506915	23.33061742671644
28	21.143443216123227	26.26144556272532	32.38681906110937	20.20829216004208
29	21.956488678048984	25.10173629452869	30.76447505982219	22.17729996760014
30	23.77205633314403	25.054128337240662	30.394448089326637	20.779367240288675
31	26.740757774680645	25.018826449159036	25.754435511421867	22.485980264738448
32	28.304363254591635	24.887537221267532	25.51562429974793	21.292475224392906
33	25.32938020757951	25.73346009196858	25.160511550439825	23.776648150012086
34	23.75905230777369	25.920585812975666	27.873540628762996	22.446821250487652
35	22.275932193926018	25.35053929970752	27.853703979892984	24.519824526473478
36	26.139009357755434	25.082156787403285	26.238119133035585	22.540714721805692
37	21.68164088759453	27.954503543780586	26.352877820202085	24.010977748422803
38	22.638061239408515	28.268473613950896	24.969161357914125	24.124303788726465
39	21.65993077744235	26.86896130163682	25.049169175023163	26.421938745897673
40	22.71369764685916	24.789492747500766	27.824426555542246	24.672383050097825
41	20.619388340605546	24.54208565464982	28.09475599819854	26.743770006546093
42	23.47446986555895	26.16123375139683	25.207237878889178	25.157058504155046
43	22.058831092404255	25.49876314820842	27.388828440170833	25.053577319216497
44	22.807664587247093	26.697227350771463	27.912773112083677	22.582334949897767
45	21.317491442690084	27.12944588892799	27.93205874292952	23.621003925452406
46	24.10024266833784	26.01407520440932	27.562839932202742	22.322842195050093
47	22.564996249403983	26.22412327522175	27.303971664449122	23.906908810925145
48	23.16924261470542	24.62220367536369	27.772704330340446	24.435849379590437
49	23.257956516596295	23.770623686281194	28.10787022717371	24.863549569948802
50	21.520302810118455	24.96280628336873	29.62886364654912	23.888027259963692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2988.0
1	3193.5
2	3399.0
3	21244.0
4	39089.0
5	27024.0
6	14959.0
7	15166.0
8	15373.0
9	16018.5
10	16664.0
11	16442.0
12	16220.0
13	15698.5
14	15177.0
15	14409.5
16	13642.0
17	13001.0
18	12360.0
19	11907.5
20	11455.0
21	11176.0
22	10897.0
23	11041.0
24	11185.0
25	12970.5
26	14756.0
27	17740.0
28	20724.0
29	23518.0
30	26312.0
31	31481.0
32	36650.0
33	43023.0
34	49396.0
35	55418.5
36	61441.0
37	68117.0
38	74793.0
39	86502.0
40	98211.0
41	116185.0
42	134159.0
43	150938.5
44	167718.0
45	177565.5
46	187413.0
47	206041.0
48	224669.0
49	246642.0
50	268615.0
51	254920.5
52	241226.0
53	212375.0
54	183524.0
55	165147.5
56	146771.0
57	137303.0
58	127835.0
59	121754.0
60	115673.0
61	106645.5
62	97618.0
63	88837.0
64	80056.0
65	67827.5
66	55599.0
67	47349.0
68	39099.0
69	35231.0
70	31363.0
71	27337.0
72	23311.0
73	18625.5
74	13940.0
75	11431.0
76	8922.0
77	6592.0
78	4262.0
79	3348.5
80	2435.0
81	1913.0
82	1391.0
83	1000.5
84	610.0
85	410.5
86	211.0
87	145.5
88	80.0
89	47.5
90	15.0
91	15.5
92	16.0
93	12.0
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2722234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.357163803444692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.02795728973884	19.00539736954355
2	7.2050348860912035	3.509884298601138
3	2.709581711682906	1.9799317677083577
4	1.5433995537748344	1.5037134298182833
5	1.0166523140377015	1.2381383472083693
6	0.7474039987292846	1.0922784974639255
7	0.5959611765843502	1.0161146798991054
8	0.49428526110164606	0.9631509656224979
9	0.4422307584105174	0.9694338319373894
>10	5.45974065148512	31.440109359857765
>50	1.4309797722576498	24.297606154220308
>100	0.31946456279967034	10.304655258031877
>500	0.004263036928706747	0.6987757553367612
>1k	0.002892775058765293	1.0962802568203223
>5k	0.0	0.0
>10k+	1.5225131888238385E-4	0.8845300279303947
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23852	0.8761921274952851	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	3265	0.11993825659366536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06182422231152796	0.0
2	0.0	0.0	0.0	0.20633788278303775	0.0
3	0.0	0.0	0.0	0.392398302276733	0.0
4	0.0	0.0	0.0	0.5931158012132682	0.0
5	0.0	0.0	0.0	0.9169674612836369	0.0
6	0.0	0.0	0.0	1.7765188444490811	0.0
7	0.0	0.0	0.0	2.320851183256105	0.0
8	0.0	0.0	0.0	4.021660151184652	0.0
9	0.0	0.0	0.0	5.0651413508170124	0.0
10	0.0	0.0	0.0	6.434421140871799	0.0
11	0.0	0.0	0.0	7.098103983713377	0.0
12	0.0	0.0	0.0	7.723582910212715	0.0
13	0.0	0.0	0.0	8.111756740970835	0.0
14	0.0	0.0	0.0	8.313392603280981	0.0
15	0.0	0.0	0.0	8.446334885244987	0.0
16	0.0	0.0	0.0	8.6730604349222	0.0
17	0.0	0.0	0.0	8.874108544673236	0.0
18	0.0	0.0	0.0	9.249094677386294	0.0
19	0.0	0.0	0.0	9.394710373906138	0.0
20	0.0	0.0	0.0	9.589293205506948	0.0
21	0.0	0.0	0.0	9.765802645915082	0.0
22	0.0	0.0	0.0	9.948189611914332	0.0
23	0.0	0.0	0.0	10.167274378323098	0.0
24	0.0	0.0	0.0	10.332359378363506	0.0
25	0.0	0.0	0.0	10.468240423123067	0.0
26	0.0	0.0	0.0	10.610954091382299	0.0
27	0.0	0.0	0.0	10.79245942854288	0.0
28	0.0	0.0	0.0	10.939507771925559	0.0
29	0.0	0.0	0.0	11.098972388119464	0.0
30	0.0	0.0	0.0	11.323383662095177	0.0
31	0.0	0.0	0.0	11.487293157017362	0.0
32	0.0	0.0	0.0	11.649476128797158	0.0
33	0.0	0.0	0.0	11.817279484423455	0.0
34	0.0	0.0	0.0	11.972115549214358	0.0
35	0.0	0.0	0.0	12.153988231724385	0.0
36	0.0	0.0	0.0	12.308383482095955	0.0
37	0.0	0.0	0.0	12.47640724493192	0.0
38	0.0	0.0	0.0	12.679622692244678	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15130	0.0	42.05155	1
TACGTAC	65	0.0	40.615387	21
CGGTCTA	690	0.0	38.89855	31
ACGGTCT	690	0.0	38.89855	30
CGACGGT	700	0.0	38.342857	28
TAGGGTA	1685	0.0	38.12463	5
TCACGAC	710	0.0	38.112675	25
ACGGGCT	920	0.0	37.543476	5
CGCATCG	300	0.0	37.4	21
ATGGTCG	300	0.0	37.4	44
CGTACGA	65	1.0913936E-11	37.23077	23
AAGGGAT	2900	0.0	37.096554	5
CGCTGAA	2055	0.0	36.934307	37
TCGTTTA	310	0.0	36.903225	38
TAGCATA	2210	0.0	36.73303	30
TAGGGCA	1690	0.0	36.710056	5
TAAGGGA	2735	0.0	36.680073	4
AGGGATT	2885	0.0	36.67938	6
CTATCCG	30	1.3015632E-4	36.666668	28
GCGTAAG	120	0.0	36.666668	1
>>END_MODULE