FastQCFastQC Report
Thu 26 May 2016
SRR1547239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547239_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2518839
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140200.5566056425202246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA48050.19076249017900707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29270.11620433064598412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG408.3200575E-944.0000041
CGTAGAT207.8582694E-444.00000443
TACTCGA207.8582694E-444.00000411
ACGTCGT207.8582694E-444.00000427
TACGTTA207.8582694E-444.0000041
TACCGTT207.8582694E-444.00000413
AGTCGAC207.8582694E-444.00000444
TCTACGA502.7284841E-1144.013
CGCTAAT254.4443572E-544.013
CGGTCTA5650.042.05309731
CACGACG5950.041.04201526
CGTTTTT85400.040.9344251
AACGTTG650.040.6153871
ACGTAGG3100.039.7419362
TACGGGA5550.039.639644
CAACCCG1900.039.3684223
CGACGGT6100.039.31147428
CCGAACA452.3528628E-839.1111113
ACGGTAC452.3528628E-839.1111113
TTGGGAC12050.039.070545
Produced by FastQC (version 0.11.5)