##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547239_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2518839
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35986301625471	31.0	31.0	33.0	30.0	34.0
2	31.74649749348807	31.0	31.0	34.0	30.0	34.0
3	31.873050242591923	31.0	31.0	34.0	30.0	34.0
4	35.55630907731697	37.0	35.0	37.0	33.0	37.0
5	35.428693139974406	37.0	35.0	37.0	33.0	37.0
6	35.463109392859174	37.0	35.0	37.0	33.0	37.0
7	35.972109769620054	37.0	35.0	37.0	35.0	37.0
8	35.963411714682834	37.0	35.0	37.0	35.0	37.0
9	37.6663220634586	39.0	37.0	39.0	35.0	39.0
10	37.025854371795894	39.0	37.0	39.0	33.0	39.0
11	36.77316454128271	39.0	35.0	39.0	32.0	39.0
12	36.063449470172564	37.0	35.0	39.0	32.0	39.0
13	35.753110063803206	37.0	35.0	39.0	31.0	39.0
14	36.759341903154585	39.0	35.0	41.0	31.0	41.0
15	37.024396160294486	39.0	35.0	41.0	32.0	41.0
16	37.14152035918136	38.0	35.0	41.0	32.0	41.0
17	37.07608267142124	38.0	35.0	41.0	32.0	41.0
18	37.06563698592883	38.0	35.0	41.0	32.0	41.0
19	37.0202513935984	38.0	35.0	41.0	32.0	41.0
20	36.85528491499457	38.0	35.0	41.0	32.0	41.0
21	36.72091943947191	38.0	35.0	41.0	31.0	41.0
22	36.64043593099837	38.0	35.0	41.0	31.0	41.0
23	36.57168520893951	38.0	35.0	40.0	31.0	41.0
24	36.52019243786522	38.0	35.0	40.0	31.0	41.0
25	36.330894511320494	38.0	35.0	40.0	31.0	41.0
26	36.21056288234381	38.0	35.0	40.0	31.0	41.0
27	36.10543548039394	38.0	35.0	40.0	30.0	41.0
28	36.20632918578758	38.0	35.0	40.0	31.0	41.0
29	36.14149931774123	37.0	35.0	40.0	31.0	41.0
30	36.14804995476091	37.0	35.0	40.0	31.0	41.0
31	36.01038772227999	37.0	35.0	40.0	30.0	41.0
32	35.80177018062687	37.0	35.0	40.0	30.0	41.0
33	35.74586902934249	38.0	35.0	40.0	30.0	41.0
34	35.66779853734201	37.0	35.0	40.0	30.0	41.0
35	35.52660571001164	37.0	35.0	40.0	29.0	41.0
36	35.36310736811682	37.0	34.0	40.0	28.0	41.0
37	35.305742844223076	37.0	34.0	40.0	28.0	41.0
38	35.26996921994618	37.0	34.0	40.0	28.0	41.0
39	35.18517301026385	37.0	34.0	40.0	27.0	41.0
40	35.03443530928337	37.0	34.0	40.0	27.0	41.0
41	35.02977363777518	36.0	34.0	40.0	27.0	41.0
42	34.826297353661744	36.0	34.0	40.0	26.0	41.0
43	34.831467195799334	36.0	34.0	40.0	27.0	41.0
44	34.577512496828895	36.0	34.0	40.0	26.0	41.0
45	34.47064699252314	35.0	34.0	40.0	26.0	41.0
46	34.47002924760177	35.0	34.0	40.0	26.0	41.0
47	34.350551186479166	35.0	33.0	40.0	26.0	41.0
48	34.31212237066363	35.0	34.0	40.0	26.0	41.0
49	34.329994096486516	35.0	34.0	40.0	26.0	41.0
50	33.78954470690663	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	2.0
13	10.0
14	27.0
15	50.0
16	171.0
17	393.0
18	951.0
19	1922.0
20	3483.0
21	5580.0
22	8717.0
23	12522.0
24	17915.0
25	25099.0
26	34051.0
27	41243.0
28	47388.0
29	53705.0
30	64039.0
31	79169.0
32	98978.0
33	128895.0
34	219233.0
35	376380.0
36	191383.0
37	231810.0
38	328772.0
39	546945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.59743874062614	24.538964181513784	28.691393137870264	14.172203939989814
2	31.449092220661978	26.121558384636735	29.315887200412572	13.113462194288719
3	28.29140727136589	26.120407060554484	31.931536711953406	13.656648956126215
4	25.56626286952044	28.775836804178432	30.654083091456023	15.003817234845101
5	22.976815906058306	32.47154740735712	29.577793578708285	14.973843107876286
6	19.752235057500698	41.17305631681898	27.317148892803388	11.757559732876931
7	86.39496212342273	4.356451523896525	7.07794344934313	2.170642903337609
8	86.0508353253225	3.6921375284406825	6.58874981687992	3.6682773293568984
9	80.58291935292411	5.411818699011727	9.923143162385529	4.08211878567864
10	46.46343017556898	24.75644533056698	14.589102360254069	14.191022133609968
11	41.25376810506745	22.014189870809528	21.630918053912936	15.101123970210084
12	36.66776637966937	20.20998563226947	25.493530948186844	17.628717039874324
13	22.491235049163524	34.28095245468249	26.981081363278875	16.246731132875105
14	17.036301248313208	38.11069306136676	27.757272298864677	17.095733391455347
15	15.489437792570307	22.469399592431273	44.740652340225004	17.300510274773416
16	16.907551455253788	18.482046688970595	43.54148875732034	21.06891309845528
17	16.531981599459115	20.005049945629715	29.78141913794411	33.681549316967065
18	21.663988845654686	22.59104293684511	32.95700916176064	22.787959055739567
19	28.199579250599182	26.248084931192505	24.403068238978353	21.149267579229956
20	29.47052193490731	24.26987195291164	23.206207304238184	23.053398807942866
21	23.232330450656036	29.65767164951789	25.908801634403787	21.201196265422283
22	22.638326625878033	24.536502730027603	24.015310228244044	28.80986041585032
23	20.062497047250737	31.380767091505252	22.719872131565378	25.836863729678633
24	20.86639916247128	24.827748022005377	33.652250104115424	20.653602711407913
25	20.105096038293834	24.666642052151804	32.09911391716581	23.129147992388557
26	18.237370471078144	31.247729608760228	27.184071709227943	23.33082821093369
27	20.548316109128052	29.06652628453029	27.41211327917346	22.97304432716819
28	18.55386549120448	27.157908861979667	35.01510021085111	19.273125435964744
29	18.85563150324415	24.38516316445791	33.358225754008096	23.40097957828984
30	21.082212876646743	26.989855246802197	30.233293989810385	21.69463788674068
31	28.14173514067394	24.70205519288847	23.944007536805646	23.212202129631944
32	28.795012305272387	24.713727237032618	25.643560386352604	20.84770007134239
33	26.243757540676476	23.706953878354273	26.376755322591084	23.672533258378163
34	19.273562145099387	24.698164511507088	29.27007244210527	26.758200901288255
35	20.102674287638074	22.664767378939267	32.157871146190764	25.0746871872319
36	28.77476488175703	22.456496822544036	25.61279224277534	23.15594605292359
37	21.282543266957514	27.45614150011176	28.747887419561152	22.51342781336957
38	22.865216871741307	29.151406659973105	23.917527082913995	24.065849385371592
39	21.703133864451043	26.994381141470335	25.619144375642904	25.683340618435718
40	24.73822264940316	22.99436367310495	26.55088316482316	25.716530512668733
41	19.292936150345458	21.85383821673398	27.354150066756944	31.49907556616362
42	23.003891872406296	25.329685621034137	24.43586112490715	27.230561381652418
43	22.371338541288267	24.378215519134013	26.624448803595623	26.625997135982093
44	22.10026127116501	26.927207336395853	28.028190765666245	22.94434062677289
45	19.43923371045152	29.928510714658618	26.2742874792712	24.357968095618656
46	23.99899318694049	27.616493154187303	26.487520639469214	21.896993019402984
47	22.889513779959735	24.69351951434768	27.30440492623784	25.11256177945474
48	23.598530910471055	22.883241048753018	28.651533504126302	24.86669453664962
49	22.070287144196197	21.725683936130892	30.03292389866919	26.171105021003726
50	20.610209703756375	27.05631443692908	28.21954876830159	24.11392709101296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3670.0
1	3589.5
2	3509.0
3	12482.0
4	21455.0
5	14993.0
6	8531.0
7	9290.0
8	10049.0
9	11037.0
10	12025.0
11	12521.5
12	13018.0
13	12305.0
14	11592.0
15	11243.0
16	10894.0
17	10327.0
18	9760.0
19	9435.0
20	9110.0
21	8878.5
22	8647.0
23	9004.0
24	9361.0
25	9648.5
26	9936.0
27	11393.5
28	12851.0
29	16267.5
30	19684.0
31	22115.0
32	24546.0
33	30546.5
34	36547.0
35	36708.0
36	36869.0
37	42965.5
38	49062.0
39	68615.0
40	88168.0
41	121347.0
42	154526.0
43	186516.0
44	218506.0
45	231295.5
46	244085.0
47	254272.5
48	264460.0
49	259930.0
50	255400.0
51	226924.0
52	198448.0
53	171570.5
54	144693.0
55	132108.0
56	119523.0
57	117978.0
58	116433.0
59	108946.5
60	101460.0
61	92313.5
62	83167.0
63	75026.5
64	66886.0
65	54974.0
66	43062.0
67	37313.0
68	31564.0
69	28026.5
70	24489.0
71	21103.0
72	17717.0
73	14354.5
74	10992.0
75	8949.5
76	6907.0
77	5361.5
78	3816.0
79	2654.0
80	1492.0
81	1291.5
82	1091.0
83	841.0
84	591.0
85	379.5
86	168.0
87	108.0
88	48.0
89	29.0
90	10.0
91	11.5
92	13.0
93	8.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2518839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.27437262229028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70297769213143	17.53059451598335
2	8.305154504242013	3.6998421222635822
3	2.902296855013961	1.9394052482724646
4	1.4869368619768994	1.3248234291796988
5	0.9256615931166282	1.0309265623611314
6	0.6495169809662508	0.8680549955128375
7	0.5081468795141413	0.7923057058806419
8	0.4192211206829191	0.747030996258037
9	0.3314828939538004	0.6645216148057875
>10	3.5933145394970194	18.490572096636313
>50	1.237895986647132	20.038312728257203
>100	0.9268213291300186	30.065631820412868
>500	0.007705573127095281	1.1545968098162993
>1k	0.002687990625730912	1.0937655321367017
>5k	0.0	0.0
>10k+	1.7919937504872748E-4	0.5596158222230698
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14020	0.5566056425202246	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4805	0.19076249017900707	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2927	0.11620433064598412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08059268575720799	0.0
2	0.0	0.0	0.0	0.345317823012904	0.0
3	0.0	0.0	0.0	0.5881281018755069	0.0
4	0.0	0.0	0.0	0.8757606182848526	0.0
5	0.0	0.0	0.0	1.4381625820467288	0.0
6	0.0	0.0	0.0	2.506670732031702	0.0
7	0.0	0.0	0.0	3.159550888325931	0.0
8	0.0	0.0	0.0	5.058243103270991	0.0
9	0.0	0.0	0.0	6.179473956056738	0.0
10	0.0	0.0	0.0	7.7192309631540565	0.0
11	0.0	0.0	0.0	8.551677975448213	0.0
12	0.0	0.0	0.0	9.370189996264152	0.0
13	0.0	0.0	0.0	9.799395673959312	0.0
14	0.0	0.0	0.0	9.987458507669604	0.0
15	0.0	0.0	0.0	10.152494859734981	0.0
16	0.0	0.0	0.0	10.47081611806074	0.0
17	0.0	0.0	0.0	10.80204014627374	0.0
18	0.0	0.0	0.0	11.386674575072087	0.0
19	0.0	0.0	0.0	11.59399231153718	0.0
20	0.0	0.0	0.0	11.87606671168741	0.0
21	0.0	0.0	0.0	12.125110020926307	0.0
22	0.0	0.0	0.0	12.359305219587277	0.0
23	0.0	0.0	0.0	12.633558556144319	0.0
24	0.0	0.0	0.0	12.847784237102887	0.0
25	0.0	0.0	0.0	13.02588216237719	0.0
26	0.0	0.0	0.0	13.189092276243143	0.0
27	0.0	0.0	0.0	13.392717835478965	0.0
28	0.0	0.0	0.0	13.579788148428701	0.0
29	0.0	0.0	0.0	13.769994826981796	0.0
30	0.0	0.0	0.0	14.049012263189509	0.0
31	0.0	0.0	0.0	14.264111362417367	0.0
32	0.0	0.0	0.0	14.473017132099352	0.0
33	0.0	0.0	0.0	14.667352697016364	0.0
34	0.0	0.0	0.0	14.851008738549785	0.0
35	0.0	0.0	0.0	15.068013477637912	0.0
36	0.0	0.0	0.0	15.257465840412983	0.0
37	0.0	0.0	0.0	15.45382614768153	0.0
38	0.0	0.0	0.0	15.652211197301614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	40	8.3200575E-9	44.000004	1
CGTAGAT	20	7.8582694E-4	44.000004	43
TACTCGA	20	7.8582694E-4	44.000004	11
ACGTCGT	20	7.8582694E-4	44.000004	27
TACGTTA	20	7.8582694E-4	44.000004	1
TACCGTT	20	7.8582694E-4	44.000004	13
AGTCGAC	20	7.8582694E-4	44.000004	44
TCTACGA	50	2.7284841E-11	44.0	13
CGCTAAT	25	4.4443572E-5	44.0	13
CGGTCTA	565	0.0	42.053097	31
CACGACG	595	0.0	41.042015	26
CGTTTTT	8540	0.0	40.934425	1
AACGTTG	65	0.0	40.615387	1
ACGTAGG	310	0.0	39.741936	2
TACGGGA	555	0.0	39.63964	4
CAACCCG	190	0.0	39.36842	23
CGACGGT	610	0.0	39.311474	28
CCGAACA	45	2.3528628E-8	39.11111	13
ACGGTAC	45	2.3528628E-8	39.11111	13
TTGGGAC	1205	0.0	39.07054	5
>>END_MODULE