FastQCFastQC Report
Thu 26 May 2016
SRR1547238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547238_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3046705
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365871.200871105013449No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC41960.13772255600722746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT233550.042.5776061
CGGTCTA6900.040.49275231
TACGGGA7650.039.3986934
CGACGGT7350.038.91156428
CACGACG7200.038.80555326
ACGGTCT7300.038.2739730
TCACGAC7400.038.05405425
AGGGCGA36000.037.956
GTTTTTT268400.037.9016382
CTCACGA7450.037.79865624
TAATGCG1250.036.9599951
GGGCGAT72600.036.606067
GACCGAT16050.036.461069
TCTCACG7700.036.28571323
GGTACCT19500.036.2153858
ATAGGGC17400.036.1609154
TAGGGTA26000.036.1307685
CGATAGA1950.036.10256610
TAGCCGT3850.036.044
ATAGGGT16450.035.9756854
Produced by FastQC (version 0.11.5)