##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547238_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3046705 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.319926281015064 31.0 31.0 33.0 30.0 34.0 2 31.724118350808496 31.0 31.0 34.0 30.0 34.0 3 31.83590304935988 31.0 31.0 34.0 30.0 34.0 4 35.549613763065345 37.0 35.0 37.0 33.0 37.0 5 35.446883108144704 37.0 35.0 37.0 33.0 37.0 6 35.47835809505679 37.0 35.0 37.0 33.0 37.0 7 35.967814737560744 37.0 35.0 37.0 35.0 37.0 8 35.95684058679787 37.0 35.0 37.0 35.0 37.0 9 37.65777520304723 39.0 37.0 39.0 35.0 39.0 10 36.93196453217492 39.0 37.0 39.0 32.0 39.0 11 36.80826236869011 39.0 37.0 39.0 32.0 39.0 12 36.53907385191543 39.0 35.0 39.0 32.0 39.0 13 36.476867304186 39.0 35.0 39.0 32.0 39.0 14 37.55908005533847 40.0 36.0 41.0 32.0 41.0 15 37.67139614764147 40.0 36.0 41.0 32.0 41.0 16 37.67861017065978 40.0 36.0 41.0 32.0 41.0 17 37.59350347342457 40.0 36.0 41.0 32.0 41.0 18 37.48346065667664 39.0 36.0 41.0 32.0 41.0 19 37.39303148811585 39.0 36.0 41.0 32.0 41.0 20 37.18495423744668 39.0 35.0 41.0 32.0 41.0 21 37.08902863913638 39.0 35.0 41.0 32.0 41.0 22 37.01705087955677 39.0 35.0 41.0 32.0 41.0 23 36.90064512317405 39.0 35.0 41.0 31.0 41.0 24 36.83598083831549 38.0 35.0 41.0 31.0 41.0 25 36.66110043473195 38.0 35.0 41.0 31.0 41.0 26 36.56841604290537 38.0 35.0 41.0 31.0 41.0 27 36.471816273646446 38.0 35.0 40.0 31.0 41.0 28 36.50933188477388 38.0 35.0 40.0 31.0 41.0 29 36.3963403086285 38.0 35.0 40.0 30.0 41.0 30 36.32587106398552 38.0 35.0 40.0 30.0 41.0 31 36.16607318398073 38.0 35.0 40.0 30.0 41.0 32 35.87664969204435 38.0 35.0 40.0 29.0 41.0 33 35.67082602352377 38.0 35.0 41.0 27.0 41.0 34 35.452989048824875 38.0 35.0 41.0 26.0 41.0 35 35.188284064259584 38.0 34.0 40.0 24.0 41.0 36 35.02792984552164 38.0 34.0 40.0 24.0 41.0 37 34.975333680156105 38.0 34.0 40.0 23.0 41.0 38 34.88894625505259 38.0 34.0 40.0 23.0 41.0 39 34.77184072629283 38.0 34.0 40.0 23.0 41.0 40 34.64348599552631 38.0 34.0 40.0 23.0 41.0 41 34.58377230483424 38.0 34.0 40.0 23.0 41.0 42 34.38194705427667 37.0 33.0 40.0 22.0 41.0 43 34.3815151122278 37.0 33.0 40.0 23.0 41.0 44 34.02987686697596 37.0 33.0 40.0 21.0 41.0 45 33.93993511022564 37.0 33.0 40.0 20.0 41.0 46 33.950451717511214 36.0 33.0 40.0 21.0 41.0 47 33.860702627920986 36.0 33.0 40.0 21.0 41.0 48 33.799856894579555 36.0 33.0 40.0 21.0 41.0 49 33.77115342640656 36.0 33.0 40.0 21.0 41.0 50 33.25300545999695 35.0 32.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 2.0 11 2.0 12 6.0 13 14.0 14 35.0 15 74.0 16 256.0 17 617.0 18 1499.0 19 2879.0 20 5097.0 21 8230.0 22 12622.0 23 17916.0 24 26065.0 25 38740.0 26 56250.0 27 68668.0 28 71501.0 29 72903.0 30 78684.0 31 91305.0 32 110269.0 33 137071.0 34 205789.0 35 290630.0 36 246687.0 37 309981.0 38 451356.0 39 741555.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.064768331689486 23.115726662082476 24.647250061952175 18.172254944275863 2 36.66902440505398 24.022082873136714 25.894466316889886 13.414426404919414 3 28.548251307560136 24.817499561001146 33.282743160233764 13.351505971204958 4 24.921546391921765 27.060086224298054 33.21145302876386 14.806914355016321 5 22.072468453624488 30.48444138831951 32.57942597002335 14.863664188032647 6 19.27977930255801 39.81658874095129 29.98495095521227 10.91868100127843 7 83.71260099025012 3.393764739283915 10.966437512000669 1.9271967584652927 8 83.07082569530033 3.0364607009868037 10.77285132626887 3.1198622774439926 9 77.49601618798013 4.646790549134228 13.941520429447552 3.9156728334380912 10 33.99488956101756 26.82130367068686 24.131742324905105 15.052064443390483 11 30.906832135044255 25.345315677100345 27.604215045434326 16.143637142421074 12 27.313507543395243 21.94859692684392 31.338052092342384 19.399843437418458 13 23.326905624272783 25.54927372358006 32.91864489669988 18.20517575544728 14 20.18606986892397 28.51047278945615 32.35715305551407 18.946304286105807 15 19.146553407697827 22.795840096103824 38.2953387347971 19.76226776140125 16 21.410802818126466 21.352346223214916 36.94673425881403 20.29011669984459 17 21.114613984616167 22.020674794573154 32.27237294060304 24.592338280207635 18 23.40869890586716 22.452157330624395 33.39663669439608 20.74250706911237 19 25.78447864168011 26.130458971249265 28.653611032246314 19.43145135482431 20 26.828688698118132 24.45714304469911 27.926727398944106 20.787440858238654 21 24.646134102251448 26.599752847748633 29.474826082603993 19.279286967395926 22 23.25791305689261 22.689988036255563 29.60214395551916 24.44995495133267 23 21.399249352989543 27.278190701101686 28.87204373249133 22.450516213417444 24 21.084220493943455 25.524230275002008 32.937386455203246 20.45416277585129 25 22.81592737071689 25.16860017625599 30.556978768866692 21.45849368416043 26 20.42396621924341 26.55997216665217 29.979830669526585 23.03623094457783 27 22.02704232933612 24.535457157814754 30.327058248173028 23.110442264676102 28 20.836280506317483 25.84884325853668 32.74091190318721 20.573964331958624 29 21.594706412337263 24.542021626642555 31.550806527051357 22.31246543396883 30 23.72576275024986 24.464593716818662 31.259147177032236 20.55049635589924 31 25.63635140258082 24.6431472689348 27.4181779988545 22.30232332962988 32 25.748899220633437 25.148841125084314 27.417062039153773 21.685197615128473 33 23.93146038096895 26.447588460320247 26.301988541719663 23.318962616991143 34 21.45284824096852 27.074790634472322 27.983805455401818 23.488555669157336 35 21.10804951578837 28.2016145311082 27.880349426675703 22.809986526427732 36 24.1195324128854 28.24461180193028 24.987092613167338 22.648763172016984 37 21.587387029594264 30.385580487772856 25.76901931759064 22.258013165042236 38 21.96815904395076 30.364541365179758 25.396945224430983 22.270354366438497 39 21.519083731441015 29.43576749307859 25.006753197306598 24.038395578173795 40 23.026351418991993 27.061235006342915 28.030413184079194 21.882000390585894 41 21.027766062024384 26.1430299290545 27.92229638248534 24.906907626435775 42 23.268317739984674 27.661063345483072 25.874674443374072 23.195944471158185 43 22.02897884764032 26.332841545210318 27.616720358551287 24.021459248598077 44 22.89630929151329 26.982034689935517 28.281372827365956 21.840283191185232 45 21.647025228894822 26.971203316369653 28.201811465173034 23.179959989562494 46 23.771352986258925 26.320730100223027 27.515200848129375 22.392716065388672 47 22.705874050818835 26.73032013273356 27.774759945580552 22.78904587086705 48 22.706628964734033 26.110010650850672 27.99332393520213 23.190036449213167 49 23.104304486322107 24.967530496060498 27.97431979794565 23.95384521967174 50 21.69379706929289 25.18163064687917 29.795270628433013 23.329301655394925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2457.0 1 3066.0 2 3675.0 3 31474.0 4 59273.0 5 40982.5 6 22692.0 7 22753.5 8 22815.0 9 23525.5 10 24236.0 11 24077.0 12 23918.0 13 23131.5 14 22345.0 15 21081.0 16 19817.0 17 18594.0 18 17371.0 19 16797.0 20 16223.0 21 15871.0 22 15519.0 23 16059.5 24 16600.0 25 17941.0 26 19282.0 27 21982.0 28 24682.0 29 27984.5 30 31287.0 31 35514.5 32 39742.0 33 47942.5 34 56143.0 35 62919.5 36 69696.0 37 77269.0 38 84842.0 39 99766.5 40 114691.0 41 136099.0 42 157507.0 43 180523.0 44 203539.0 45 227469.5 46 251400.0 47 258105.0 48 264810.0 49 252699.5 50 240589.0 51 224205.5 52 207822.0 53 192582.5 54 177343.0 55 164445.5 56 151548.0 57 148873.0 58 146198.0 59 138310.5 60 130423.0 61 119409.0 62 108395.0 63 99882.0 64 91369.0 65 77057.5 66 62746.0 67 53742.0 68 44738.0 69 40259.5 70 35781.0 71 31283.0 72 26785.0 73 22037.0 74 17289.0 75 13518.5 76 9748.0 77 7849.5 78 5951.0 79 4568.5 80 3186.0 81 2195.0 82 1204.0 83 910.0 84 616.0 85 425.0 86 234.0 87 162.5 88 91.0 89 65.5 90 40.0 91 38.5 92 37.0 93 22.0 94 7.0 95 5.0 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3046705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.396231941202984 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19401163097716 20.37631035483194 2 6.777819505546468 3.578177914480286 3 2.8590018841940688 2.26400630566569 4 1.6253502462838 1.7161248834639435 5 1.1414719466647143 1.5065279129269142 6 0.8801499987901582 1.3939586106668724 7 0.7020183760536333 1.2971447916908856 8 0.6141481445362139 1.2968957495549887 9 0.5123241736406788 1.2171084944854063 >10 6.562737292316837 40.11353659579423 >50 1.0181751028685406 17.887558603477043 >100 0.10614953563608208 4.258175655090065 >500 0.0038850384385539106 0.6735244031458014 >1k 0.0026318002325686667 1.2106237258115387 >5k 0.0 0.0 >10k+ 1.2532382059850792E-4 1.2103259989142736 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36587 1.200871105013449 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC 4196 0.13772255600722746 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05139979092166783 0.0 2 0.0 0.0 0.0 0.19181377914829298 0.0 3 0.0 0.0 0.0 0.3937040179472578 0.0 4 0.0 0.0 0.0 0.5606712825823308 0.0 5 0.0 0.0 0.0 0.9025159967899747 0.0 6 0.0 0.0 0.0 1.7170024665991621 0.0 7 0.0 0.0 0.0 2.2371053318256937 0.0 8 0.0 0.0 0.0 3.7394824900999604 0.0 9 0.0 0.0 0.0 4.638223917313951 0.0 10 0.0 0.0 0.0 5.846709806167647 0.0 11 0.0 0.0 0.0 6.388967753688001 0.0 12 0.0 0.0 0.0 6.904409846046795 0.0 13 0.0 0.0 0.0 7.224001010928199 0.0 14 0.0 0.0 0.0 7.390640052121882 0.0 15 0.0 0.0 0.0 7.519599042243998 0.0 16 0.0 0.0 0.0 7.724180713262361 0.0 17 0.0 0.0 0.0 7.910316226874607 0.0 18 0.0 0.0 0.0 8.25938185679283 0.0 19 0.0 0.0 0.0 8.392410817588182 0.0 20 3.282234413899606E-5 0.0 0.0 8.570340745165678 0.0 21 3.282234413899606E-5 0.0 0.0 8.723161579476844 0.0 22 3.282234413899606E-5 0.0 0.0 8.883695664660674 0.0 23 3.282234413899606E-5 0.0 0.0 9.068386995130805 0.0 24 3.282234413899606E-5 0.0 0.0 9.224752642608982 0.0 25 3.282234413899606E-5 0.0 0.0 9.357190801209832 0.0 26 6.564468827799212E-5 0.0 0.0 9.490121294972766 0.0 27 6.564468827799212E-5 0.0 0.0 9.659156367288595 0.0 28 6.564468827799212E-5 0.0 0.0 9.80715231701133 0.0 29 6.564468827799212E-5 0.0 0.0 9.959447993816271 0.0 30 6.564468827799212E-5 0.0 0.0 10.172268073213521 0.0 31 6.564468827799212E-5 0.0 0.0 10.335066900142941 0.0 32 6.564468827799212E-5 0.0 0.0 10.517427844179203 0.0 33 6.564468827799212E-5 0.0 0.0 10.690926755297937 0.0 34 6.564468827799212E-5 0.0 0.0 10.847784737938198 0.0 35 6.564468827799212E-5 0.0 0.0 11.030408260727572 0.0 36 6.564468827799212E-5 0.0 0.0 11.185723593193302 0.0 37 9.846703241698819E-5 0.0 0.0 11.357154696631278 0.0 38 1.3128937655598424E-4 0.0 0.0 11.55586116804876 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 23355 0.0 42.577606 1 CGGTCTA 690 0.0 40.492752 31 TACGGGA 765 0.0 39.398693 4 CGACGGT 735 0.0 38.911564 28 CACGACG 720 0.0 38.805553 26 ACGGTCT 730 0.0 38.27397 30 TCACGAC 740 0.0 38.054054 25 AGGGCGA 3600 0.0 37.95 6 GTTTTTT 26840 0.0 37.901638 2 CTCACGA 745 0.0 37.798656 24 TAATGCG 125 0.0 36.959995 1 GGGCGAT 7260 0.0 36.60606 7 GACCGAT 1605 0.0 36.46106 9 TCTCACG 770 0.0 36.285713 23 GGTACCT 1950 0.0 36.215385 8 ATAGGGC 1740 0.0 36.160915 4 TAGGGTA 2600 0.0 36.130768 5 CGATAGA 195 0.0 36.102566 10 TAGCCGT 385 0.0 36.0 44 ATAGGGT 1645 0.0 35.975685 4 >>END_MODULE