FastQCFastQC Report
Thu 26 May 2016
SRR1547237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547237_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences543034
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49390.9095194776017708No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC6450.11877709314702209No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC5460.10054619047794427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTG600.044.0000041
TTTAGCG254.4397286E-544.01
GTTTACG351.4442048E-744.01
CTAAGCG207.8528083E-444.01
TACGCGG254.4397286E-544.02
GTATGCG207.8528083E-444.01
TGACACG207.8528083E-444.019
GCGATAA551.8189894E-1244.09
ATAACGG254.4397286E-544.02
ATACCGG207.8528083E-444.02
CGTTTTT25850.042.382981
TTGCTAG800.041.251
ACGGGTA603.6379788E-1240.3333365
TATGGGC2300.040.173914
GTAGCAT3350.040.05970429
AATACGG557.8216544E-1140.02
CGACGGT1050.039.8095228
CGGTCTA1050.039.8095231
CGCATGG501.3442332E-939.62
TAGCATA3350.039.40298530
Produced by FastQC (version 0.11.5)