##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547237_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	543034
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31088845265674	31.0	31.0	33.0	30.0	34.0
2	31.706622421432176	31.0	31.0	34.0	30.0	34.0
3	31.84087000077343	31.0	31.0	34.0	30.0	34.0
4	35.54570247903446	37.0	35.0	37.0	33.0	37.0
5	35.43863735972333	37.0	35.0	37.0	33.0	37.0
6	35.46907560115941	37.0	35.0	37.0	33.0	37.0
7	35.94804929341441	37.0	35.0	37.0	35.0	37.0
8	35.942510045411524	37.0	35.0	37.0	35.0	37.0
9	37.66418861434091	39.0	37.0	39.0	35.0	39.0
10	36.98500830518899	39.0	37.0	39.0	32.0	39.0
11	36.755659866601356	39.0	37.0	39.0	32.0	39.0
12	36.36186131991735	38.0	35.0	39.0	32.0	39.0
13	36.233828821031466	39.0	35.0	39.0	32.0	39.0
14	37.27057421818892	39.0	35.0	41.0	32.0	41.0
15	37.42302691912477	39.0	35.0	41.0	32.0	41.0
16	37.44758523407374	39.0	35.0	41.0	32.0	41.0
17	37.334345547424284	39.0	35.0	41.0	32.0	41.0
18	37.28435604400461	39.0	35.0	41.0	32.0	41.0
19	37.22957678524733	39.0	35.0	41.0	32.0	41.0
20	37.054900798108406	39.0	35.0	41.0	32.0	41.0
21	36.97316374297005	39.0	35.0	41.0	31.0	41.0
22	36.90115536043783	39.0	35.0	41.0	31.0	41.0
23	36.79019177436404	38.0	35.0	41.0	31.0	41.0
24	36.727044715432186	38.0	35.0	41.0	31.0	41.0
25	36.56560362702888	38.0	35.0	40.0	31.0	41.0
26	36.44263158476265	38.0	35.0	40.0	31.0	41.0
27	36.36473590972204	38.0	35.0	40.0	30.0	41.0
28	36.416167311807364	38.0	35.0	40.0	31.0	41.0
29	36.30517426164844	38.0	35.0	40.0	30.0	41.0
30	36.25269872604662	38.0	35.0	40.0	30.0	41.0
31	36.140810704302126	38.0	35.0	40.0	30.0	41.0
32	35.89319084992837	38.0	35.0	40.0	29.0	41.0
33	35.77681692122408	38.0	35.0	40.0	29.0	41.0
34	35.602750472346116	38.0	35.0	40.0	27.0	41.0
35	35.387334863010416	38.0	34.0	40.0	26.0	41.0
36	35.21964186404534	38.0	34.0	40.0	26.0	41.0
37	35.1793349955988	38.0	34.0	40.0	26.0	41.0
38	35.10177079151582	38.0	34.0	40.0	26.0	41.0
39	34.92960661763352	37.0	34.0	40.0	25.0	41.0
40	34.81493792285566	37.0	34.0	40.0	24.0	41.0
41	34.773671630137336	37.0	34.0	40.0	24.0	41.0
42	34.59372157176162	37.0	34.0	40.0	24.0	41.0
43	34.60439309509165	37.0	34.0	40.0	24.0	41.0
44	34.36120758552872	36.0	33.0	40.0	23.0	41.0
45	34.26710298066051	36.0	33.0	40.0	23.0	41.0
46	34.24900282486916	36.0	33.0	40.0	23.0	41.0
47	34.12387437987309	36.0	33.0	40.0	23.0	41.0
48	34.083808748623476	36.0	33.0	40.0	23.0	41.0
49	34.05353808417153	36.0	33.0	40.0	24.0	41.0
50	33.53018227219658	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	24.0
16	50.0
17	120.0
18	240.0
19	544.0
20	886.0
21	1385.0
22	2160.0
23	3096.0
24	4466.0
25	6180.0
26	8520.0
27	10496.0
28	11526.0
29	12333.0
30	13784.0
31	16871.0
32	20533.0
33	26193.0
34	41130.0
35	57721.0
36	43728.0
37	55769.0
38	80019.0
39	125250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.84907758998516	23.204256087095835	27.072890463580553	15.873775859338457
2	34.172445924196275	24.53474368087449	28.189579289694567	13.103231105234665
3	28.84202462460914	25.774629212903793	32.058581967243306	13.32476419524376
4	25.44794616911648	27.735095776691697	32.015306592220746	14.801651461971074
5	22.72785866078367	31.80684818998442	30.541734035069627	14.92355911416228
6	19.424566417572382	40.8440355484187	28.523628354762316	11.207769679246603
7	85.59261482706424	3.7307056280085593	8.723210701355717	1.9534688435714889
8	85.47383773391721	3.3136046730039004	8.024175281842389	3.188382311236497
9	80.30325909611553	4.6962068673416395	10.882007388119344	4.1185266484234875
10	40.81899107606522	26.88395201773738	17.06817620996107	15.228880696236333
11	33.99289915548566	24.40068209357057	25.116291060964873	16.490127689978895
12	31.179079026359307	20.42082079575128	28.504476699433184	19.89562347845623
13	24.447088027637314	26.448067708467608	29.35304235093935	19.751801912955727
14	20.326719873893715	28.91881539645768	30.38980984616065	20.364654883487958
15	19.338383968591284	22.352375726013474	37.4053926641794	20.903847641215837
16	22.153309000909704	20.123049385489676	35.43553442325894	22.288107190341673
17	21.050799765760523	22.272822696184768	30.685739751102144	25.990637786952565
18	24.000522987510912	22.649042233083012	31.390852138171827	21.959582641234253
19	26.89923651189428	27.332542713715902	25.248511142948693	20.519709631441124
20	28.43247384141693	24.99235775292155	25.266742045617768	21.308426360043754
21	25.095297900315632	28.386620358946217	25.939628089585554	20.578453651152596
22	23.66665807297517	23.315667158962423	27.26182890942372	25.755845858638686
23	22.975172825274292	27.552234298404887	25.76892054641146	23.703672329909363
24	23.232983570089534	24.862715778385883	31.287543689713722	20.61675696181086
25	23.700725921397186	25.207629724842274	27.86271209537524	23.228932258385296
26	20.66205799268554	27.03016754015402	27.95515566244471	24.352618804715725
27	22.296762265346185	24.333098848322575	27.989775962462755	25.38036292386849
28	21.239554061071683	26.0453304949598	30.447448962680053	22.267666481288465
29	22.313704114291184	24.094808059900487	29.611957998946657	23.979529826861672
30	24.041036104553307	24.598828066014285	29.239237322156626	22.12089850727579
31	27.097566634870006	23.906237915121338	25.8897232954106	23.106472154598055
32	26.755783247457803	23.817477358692088	25.24832699241668	24.178412401433427
33	25.28773520626701	24.207508185491147	25.313516280748534	25.191240327493308
34	21.528486245796763	25.94736241193001	27.177672116294744	25.346479225978484
35	22.597111783055944	25.054232331677206	27.637127693661906	24.711528191604945
36	25.525289392561056	27.723310142643005	23.644375858601855	23.107024606194088
37	24.366798395680565	27.261092307295677	25.333036237141688	23.03907305988207
38	24.879657627330886	28.226777697160767	24.638052129332603	22.255512546175744
39	24.527193509062048	26.053248967836268	24.95571179705138	24.463845726050305
40	25.671873216041718	23.561323968664947	27.69034719741305	23.076455617880278
41	21.892920148646308	23.994998471550584	28.21941167588033	25.892669703922778
42	24.36956065366073	26.08473870881013	24.66475395647418	24.88094668105496
43	22.49140937768169	24.61024539899896	27.35850793872944	25.539837284589918
44	23.244032602010186	25.44665711539241	27.869893966123666	23.43941631647374
45	22.159754269530087	26.53314525425664	26.648239336763446	24.65886113944983
46	24.16883657376886	26.08289720349002	26.74952212936943	22.998744093371688
47	22.776842702298566	25.757871514490805	27.390365980767317	24.074919802443308
48	23.71343230810594	24.329599988214365	27.862896245907255	24.094071457772444
49	23.899056044372912	23.47072190691559	27.626078661741253	25.004143386970245
50	22.226048461054006	24.62976535539211	28.95417229860377	24.190013884950112
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	602.0
1	626.0
2	650.0
3	4338.0
4	8026.0
5	5381.0
6	2736.0
7	2809.5
8	2883.0
9	2954.5
10	3026.0
11	2966.5
12	2907.0
13	2888.5
14	2870.0
15	2727.5
16	2585.0
17	2436.0
18	2287.0
19	2187.0
20	2087.0
21	2158.5
22	2230.0
23	2161.5
24	2093.0
25	2268.5
26	2444.0
27	3013.0
28	3582.0
29	4384.5
30	5187.0
31	5858.5
32	6530.0
33	7741.0
34	8952.0
35	10318.0
36	11684.0
37	12855.5
38	14027.0
39	15841.0
40	17655.0
41	21394.5
42	25134.0
43	27527.0
44	29920.0
45	32021.5
46	34123.0
47	37092.5
48	40062.0
49	44154.5
50	48247.0
51	47479.0
52	46711.0
53	42044.0
54	37377.0
55	34200.5
56	31024.0
57	30107.0
58	29190.0
59	28225.5
60	27261.0
61	25079.0
62	22897.0
63	21295.0
64	19693.0
65	16544.5
66	13396.0
67	11682.5
68	9969.0
69	9146.0
70	8323.0
71	7233.0
72	6143.0
73	5005.5
74	3868.0
75	2965.5
76	2063.0
77	1724.5
78	1386.0
79	988.0
80	590.0
81	483.0
82	376.0
83	266.0
84	156.0
85	107.0
86	58.0
87	36.5
88	15.0
89	8.5
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	543034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.599136423787606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.25873938705176	26.416679271770988
2	8.030968610034373	6.039149687676766
3	4.407195701628064	4.971202572955318
4	3.050219917553637	4.587425392106135
5	2.41306500890809	4.536458023470176
6	1.8960340639722055	4.277354606126363
7	1.5379714414155123	4.047847862916979
8	1.2952851878856424	3.89612835896189
9	1.1200721532296365	3.7902371124389975
>10	5.9315181905470995	33.966761009235434
>50	0.03879580570147881	0.9954166169037925
>100	0.018661273628159766	1.343470841118953
>500	9.82172296218935E-4	0.2199111835861288
>1k	4.910861481094675E-4	0.9119574607320655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4939	0.9095194776017708	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	645	0.11877709314702209	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	546	0.10054619047794427	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04879989098288505	0.0
2	0.0	0.0	0.0	0.1821248761587672	0.0
3	0.0	0.0	0.0	0.3279720975113897	0.0
4	0.0	0.0	0.0	0.5134116832463529	0.0
5	0.0	0.0	0.0	0.8605354360868749	0.0
6	0.0	0.0	0.0	1.7133365498292925	0.0
7	0.0	0.0	0.0	2.2493987485129843	0.0
8	0.0	0.0	0.0	3.827200506782264	0.0
9	0.0	0.0	0.0	4.73561508119197	0.0
10	0.0	0.0	0.0	5.962241774916488	0.0
11	0.0	0.0	0.0	6.5170873278652905	0.0
12	0.0	0.0	0.0	7.05480688133708	0.0
13	0.0	0.0	0.0	7.3870144410847205	0.0
14	1.8415053201088698E-4	0.0	0.0	7.56748196245539	0.0
15	1.8415053201088698E-4	0.0	0.0	7.698044689651108	0.0
16	1.8415053201088698E-4	0.0	0.0	7.882011071129985	0.0
17	1.8415053201088698E-4	0.0	0.0	8.056954076540327	0.0
18	1.8415053201088698E-4	0.0	0.0	8.376823550643238	0.0
19	1.8415053201088698E-4	0.0	0.0	8.508675331563033	0.0
20	1.8415053201088698E-4	0.0	0.0	8.658942165683916	0.0
21	1.8415053201088698E-4	0.0	0.0	8.804421085972518	0.0
22	1.8415053201088698E-4	0.0	0.0	8.950268307325139	0.0
23	1.8415053201088698E-4	0.0	0.0	9.121528302095264	0.0
24	1.8415053201088698E-4	0.0	0.0	9.273268340472235	0.0
25	1.8415053201088698E-4	0.0	0.0	9.4029103150079	0.0
26	1.8415053201088698E-4	0.0	0.0	9.533288891671608	0.0
27	1.8415053201088698E-4	0.0	0.0	9.680425166748307	0.0
28	1.8415053201088698E-4	0.0	0.0	9.812276947668101	0.0
29	1.8415053201088698E-4	0.0	0.0	9.94652268550404	0.0
30	1.8415053201088698E-4	0.0	0.0	10.140249045179491	0.0
31	1.8415053201088698E-4	0.0	0.0	10.2892268255763	0.0
32	1.8415053201088698E-4	0.0	0.0	10.465090583646695	0.0
33	1.8415053201088698E-4	0.0	0.0	10.620513632663885	0.0
34	1.8415053201088698E-4	0.0	0.0	10.765440101356452	0.0
35	1.8415053201088698E-4	0.0	0.0	10.92694011793	0.0
36	1.8415053201088698E-4	0.0	0.0	11.082179016415179	0.0
37	1.8415053201088698E-4	0.0	0.0	11.245336387776824	0.0
38	1.8415053201088698E-4	0.0	0.0	11.4394310485163	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATTG	60	0.0	44.000004	1
TTTAGCG	25	4.4397286E-5	44.0	1
GTTTACG	35	1.4442048E-7	44.0	1
CTAAGCG	20	7.8528083E-4	44.0	1
TACGCGG	25	4.4397286E-5	44.0	2
GTATGCG	20	7.8528083E-4	44.0	1
TGACACG	20	7.8528083E-4	44.0	19
GCGATAA	55	1.8189894E-12	44.0	9
ATAACGG	25	4.4397286E-5	44.0	2
ATACCGG	20	7.8528083E-4	44.0	2
CGTTTTT	2585	0.0	42.38298	1
TTGCTAG	80	0.0	41.25	1
ACGGGTA	60	3.6379788E-12	40.333336	5
TATGGGC	230	0.0	40.17391	4
GTAGCAT	335	0.0	40.059704	29
AATACGG	55	7.8216544E-11	40.0	2
CGACGGT	105	0.0	39.80952	28
CGGTCTA	105	0.0	39.80952	31
CGCATGG	50	1.3442332E-9	39.6	2
TAGCATA	335	0.0	39.402985	30
>>END_MODULE