FastQCFastQC Report
Thu 26 May 2016
SRR1547236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547236_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2430597
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362571.4916911359637157No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT25410.10454221740584721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT297800.043.246471
TCGTTAG1000.039.6000021
GTTTTTT336950.038.802792
AGGGCGA24500.037.6244936
ACGGGTA3450.037.623195
TAATGCG651.0913936E-1137.2307661
CGACGGT2550.037.0980428
ACTACGG1200.036.6666682
TGGGCGA16050.036.3239866
TAGGGCG12050.035.9668055
CGTTAGG3650.035.561642
GGGCGAT50600.035.478267
GTAGGGT14150.035.448764
ATAGGGC15850.035.255524
AAGGGCG12050.035.2365155
ACCAACG250.002353259135.226
GCGCGAC11850.035.0886089
TAGGGTA18250.034.95895
GGCGATA11900.034.9411778
TACGGGT2650.034.8679244
Produced by FastQC (version 0.11.5)