##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547236_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2430597
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.372053450242884	31.0	31.0	33.0	30.0	34.0
2	31.77237485276251	31.0	31.0	34.0	30.0	34.0
3	31.846534822514798	31.0	31.0	34.0	30.0	34.0
4	35.57831676744438	37.0	35.0	37.0	33.0	37.0
5	35.482667426973705	37.0	35.0	37.0	33.0	37.0
6	35.51669034397722	37.0	35.0	37.0	33.0	37.0
7	36.00110055266258	37.0	35.0	37.0	35.0	37.0
8	35.99633875957224	37.0	35.0	37.0	35.0	37.0
9	37.71326015789536	39.0	37.0	39.0	35.0	39.0
10	36.996470003048636	39.0	37.0	39.0	32.0	39.0
11	36.85764114742181	39.0	37.0	39.0	32.0	39.0
12	36.61725288067088	39.0	35.0	39.0	32.0	39.0
13	36.59191013565803	39.0	35.0	39.0	32.0	39.0
14	37.7270448371326	40.0	36.0	41.0	32.0	41.0
15	37.8243744232384	40.0	36.0	41.0	33.0	41.0
16	37.81514294636256	40.0	36.0	41.0	33.0	41.0
17	37.74069909573656	40.0	36.0	41.0	32.0	41.0
18	37.549945959778604	39.0	36.0	41.0	32.0	41.0
19	37.38770145770771	39.0	36.0	41.0	32.0	41.0
20	37.10213540130264	39.0	35.0	41.0	32.0	41.0
21	37.02096686534214	39.0	35.0	41.0	32.0	41.0
22	36.94713109577606	38.0	35.0	41.0	32.0	41.0
23	36.83913499440673	38.0	35.0	41.0	32.0	41.0
24	36.78086577083737	38.0	35.0	41.0	32.0	41.0
25	36.620197424747914	38.0	35.0	40.0	31.0	41.0
26	36.53775841902216	38.0	35.0	40.0	31.0	41.0
27	36.41416738356873	38.0	35.0	40.0	31.0	41.0
28	36.44137551391695	38.0	35.0	40.0	31.0	41.0
29	36.324989292754005	38.0	35.0	40.0	31.0	41.0
30	36.211363710232504	38.0	35.0	40.0	30.0	41.0
31	35.97164688346114	38.0	35.0	40.0	30.0	41.0
32	35.520161507646066	38.0	35.0	40.0	27.0	41.0
33	35.15017257077171	38.0	34.0	40.0	24.0	41.0
34	34.77067074467713	38.0	34.0	40.0	22.0	41.0
35	34.43013136278865	38.0	34.0	40.0	19.0	41.0
36	34.25512086125343	38.0	34.0	40.0	18.0	41.0
37	34.19033184028451	38.0	34.0	40.0	18.0	41.0
38	34.12870747392513	38.0	33.0	40.0	18.0	41.0
39	34.00342508445456	38.0	33.0	40.0	18.0	41.0
40	33.910329026161065	38.0	33.0	40.0	17.0	41.0
41	33.87241858687393	38.0	33.0	40.0	17.0	41.0
42	33.67606312358651	37.0	33.0	40.0	15.0	41.0
43	33.68731879451838	37.0	33.0	40.0	15.0	41.0
44	33.430077055143244	37.0	33.0	40.0	15.0	41.0
45	33.34065499134575	37.0	33.0	40.0	15.0	41.0
46	33.33255862654319	37.0	33.0	40.0	15.0	41.0
47	33.23351423539155	37.0	33.0	40.0	15.0	41.0
48	33.16679647016762	36.0	32.0	40.0	15.0	41.0
49	33.13512647304346	36.0	32.0	40.0	15.0	41.0
50	32.647987305176464	36.0	31.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	7.0
12	7.0
13	7.0
14	30.0
15	77.0
16	178.0
17	539.0
18	1273.0
19	2572.0
20	4588.0
21	7418.0
22	11470.0
23	16689.0
24	25501.0
25	40394.0
26	61462.0
27	71003.0
28	66879.0
29	60616.0
30	62528.0
31	70671.0
32	84838.0
33	105030.0
34	155461.0
35	216555.0
36	186336.0
37	233418.0
38	343765.0
39	601283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.01822309498448	22.148426909109162	25.289219068401714	21.544130927504643
2	38.87678623811352	22.96402900192833	25.816455792548087	12.342728967410064
3	26.77506801826876	23.207425994519042	37.17909632900888	12.838409658203314
4	22.92082973853749	25.67825929185299	37.44137757102473	13.959533398584792
5	20.613742220532654	29.436512922545365	36.61804075295082	13.331704103971164
6	17.26193194511472	38.55698826255443	34.07706008030126	10.104019712029597
7	79.54255682863099	3.3634123633000454	15.121182162242444	1.9728486458265193
8	79.55230751951063	2.725297529783835	14.830265979921805	2.892128970783721
9	73.99136919859608	4.796722780452703	17.558690313532026	3.653217707419206
10	33.957212981008375	24.033190199773966	26.438031479508943	15.571565339708721
11	28.353980524126378	25.40223656986329	30.378544859555078	15.865238046455254
12	23.780988785882645	21.516771394023774	36.346502525922645	18.355737294170936
13	21.572148735475277	24.442801501030406	37.286518497307455	16.698531266186865
14	19.112176967222457	27.266346498411707	36.34625567298898	17.275220861376855
15	18.251483071854363	23.342536833543363	41.456317110569955	16.94966298403232
16	19.51376554813488	21.815298875132324	40.1592283706431	18.511707206089696
17	19.754817437855802	22.68874683873962	35.8671552709067	21.689280452497883
18	21.72017821136124	23.06951748891322	36.65371100186498	18.556593297860566
19	24.14624884339115	26.335505227728	32.24537017037378	17.272875758507066
20	24.180972822726268	24.16817761233146	32.48806774631912	19.162781818623163
21	22.982872109197864	26.14797928245612	33.40294586062601	17.466202747720004
22	21.50615671787631	23.392606836921136	33.503168151692776	21.598068293509783
23	20.010803930063272	26.520480359352046	32.86303735255166	20.60567835803303
24	20.135505803718182	24.656411572959236	35.776313391319086	19.431769232003496
25	20.41387362857767	25.13226174474831	34.38764221300364	20.06622241367039
26	18.77320674714895	26.18406095292638	34.50382766044721	20.53890463947746
27	20.17882849357586	24.433955937574183	34.50271682224573	20.88449874660423
28	20.338007493632222	25.052610531486707	36.04748133894677	18.5619006359343
29	20.53717666894183	24.531709699304326	34.59705578506021	20.334057846693632
30	20.999984777402425	24.103131864311525	34.93351633364149	19.963367024644562
31	23.521217215359023	23.612923080214447	31.973338237478284	20.892521466948246
32	23.41070938538968	23.815795049528983	32.227761327772555	20.54573423730878
33	23.540142606939778	24.04458657687803	30.14366429317571	22.271606523006486
34	20.745808540041807	25.40441710411064	31.38484084362813	22.464933512219424
35	21.61629426844516	25.592930461117163	29.831683327182578	22.959091943255096
36	23.534835268866043	26.120907744064525	27.373233818687343	22.97102316838209
37	21.447158866731094	28.93988596217308	28.005341897484442	21.60761327361138
38	22.714378401684854	28.77165568788244	27.18694213808377	21.327023772348934
39	21.935763106759367	28.30855958433257	27.15913004089119	22.59654726801687
40	22.759552488544994	26.029819011543253	29.22615308090975	21.984475419002
41	20.72108210452	26.14686844425464	29.29193938773067	23.840110063494688
42	22.069145975248055	27.551626205413733	27.957987276376954	22.421240542961257
43	21.644805782283118	26.372204030532416	29.262975310180995	22.720014877003468
44	21.782919998666994	27.457081531821192	29.48349726425236	21.27650120525945
45	20.704748668742702	27.400716778635044	29.780831622848215	22.113702929774043
46	23.11979320306904	26.80106986061449	28.48209719669694	21.597039739619525
47	21.910337254592186	26.28905573404394	29.298851269873204	22.50175574149067
48	22.532365505264757	25.503981120687634	29.424375986640317	22.539277387407292
49	22.393798725169166	24.59708458456914	29.769517530055374	23.23959916020632
50	21.13279165571257	25.053433374598917	30.877516922797156	22.93625804689136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2172.0
1	2465.0
2	2758.0
3	31939.0
4	61120.0
5	43792.0
6	26464.0
7	26684.5
8	26905.0
9	27854.5
10	28804.0
11	28573.0
12	28342.0
13	27846.0
14	27350.0
15	26289.0
16	25228.0
17	23911.5
18	22595.0
19	21324.5
20	20054.0
21	19675.5
22	19297.0
23	18901.5
24	18506.0
25	19565.5
26	20625.0
27	22258.0
28	23891.0
29	27583.5
30	31276.0
31	36175.0
32	41074.0
33	45374.5
34	49675.0
35	57337.0
36	64999.0
37	70232.5
38	75466.0
39	85031.0
40	94596.0
41	107443.5
42	120291.0
43	132929.0
44	145567.0
45	150429.5
46	155292.0
47	169324.0
48	183356.0
49	199431.0
50	215506.0
51	208809.0
52	202112.0
53	176739.5
54	151367.0
55	135176.5
56	118986.0
57	110670.0
58	102354.0
59	93740.0
60	85126.0
61	77209.0
62	69292.0
63	62320.5
64	55349.0
65	45331.0
66	35313.0
67	30379.0
68	25445.0
69	22984.0
70	20523.0
71	17403.5
72	14284.0
73	11560.5
74	8837.0
75	6894.0
76	4951.0
77	3953.0
78	2955.0
79	2171.5
80	1388.0
81	1006.0
82	624.0
83	463.0
84	302.0
85	206.5
86	111.0
87	75.0
88	39.0
89	31.0
90	23.0
91	14.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2430597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.114686318340446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5968855960178	25.033671531555985
2	7.300485296569363	4.835065611351022
3	3.3769251674546625	3.3547745292231137
4	2.131069997800338	2.8227885799833863
5	1.4588531058065173	2.415473149165977
6	1.182568635618668	2.3496233651052014
7	0.9321092397831685	2.160655356289248
8	0.7572462208341432	2.006077685493713
9	0.6715217665253068	2.0013509388981046
>10	6.265812980069185	41.976344217860465
>50	0.2808516533703343	5.919409568288086
>100	0.04216845077943753	2.419036182276967
>500	0.001750944685606313	0.3684755084764489
>1k	0.0016258772080629982	0.8356446369033703
>5k	0.0	0.0
>10k+	1.2506747754330755E-4	1.5016091391289832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36257	1.4916911359637157	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	2541	0.10454221740584721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06228922359403883	0.0
2	0.0	0.0	0.0	0.21661344928838472	0.0
3	0.0	0.0	0.0	0.44655695699451614	0.0
4	0.0	0.0	0.0	0.6125244127265853	0.0
5	0.0	0.0	0.0	0.9508775004659349	0.0
6	0.0	0.0	0.0	1.8332533118406713	0.0
7	0.0	0.0	0.0	2.407104098293547	0.0
8	0.0	0.0	0.0	3.8748504996920508	0.0
9	0.0	0.0	0.0	4.815524745566624	0.0
10	0.0	0.0	0.0	6.008894111199841	0.0
11	0.0	0.0	0.0	6.5360074088793825	0.0
12	0.0	0.0	0.0	7.07262454450491	0.0
13	0.0	0.0	0.0	7.478121630200317	0.0
14	0.0	0.0	0.0	7.690250584527176	0.0
15	0.0	0.0	0.0	7.833055006650629	0.0
16	0.0	0.0	0.0	8.037654946500798	0.0
17	0.0	0.0	0.0	8.233409322894746	0.0
18	0.0	0.0	0.0	8.60858463990534	0.0
19	0.0	0.0	0.0	8.749455380715109	0.0
20	0.0	0.0	0.0	8.937516174009925	0.0
21	0.0	0.0	0.0	9.101015100405373	0.0
22	0.0	0.0	0.0	9.274717281392185	0.0
23	0.0	0.0	0.0	9.475984706637917	0.0
24	0.0015222597575821908	0.0	0.0	9.650962294448648	0.0
25	0.0015222597575821908	0.0	0.0	9.805615657387877	0.0
26	0.0015222597575821908	0.0	0.0	9.959446177214899	0.0
27	0.0015222597575821908	0.0	0.0	10.176759043148659	0.0
28	0.0015222597575821908	0.0	0.0	10.342891067503169	0.0
29	0.0015222597575821908	0.0	0.0	10.518033223936342	0.0
30	0.0015222597575821908	0.0	0.0	10.758097701922614	0.0
31	0.0015222597575821908	0.0	0.0	10.940563162054426	0.0
32	0.0015222597575821908	0.0	0.0	11.12734854852532	0.0
33	0.0015222597575821908	0.0	0.0	11.32446061605441	0.0
34	0.0015222597575821908	0.0	0.0	11.519721286581033	0.0
35	0.0015222597575821908	0.0	0.0	11.722264118650685	0.0
36	0.0015222597575821908	0.0	0.0	11.909831206078177	0.0
37	0.0015222597575821908	0.0	0.0	12.11113977347952	0.0
38	0.0015222597575821908	0.0	0.0	12.325366977742505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29780	0.0	43.24647	1
TCGTTAG	100	0.0	39.600002	1
GTTTTTT	33695	0.0	38.80279	2
AGGGCGA	2450	0.0	37.624493	6
ACGGGTA	345	0.0	37.62319	5
TAATGCG	65	1.0913936E-11	37.230766	1
CGACGGT	255	0.0	37.09804	28
ACTACGG	120	0.0	36.666668	2
TGGGCGA	1605	0.0	36.323986	6
TAGGGCG	1205	0.0	35.966805	5
CGTTAGG	365	0.0	35.56164	2
GGGCGAT	5060	0.0	35.47826	7
GTAGGGT	1415	0.0	35.44876	4
ATAGGGC	1585	0.0	35.25552	4
AAGGGCG	1205	0.0	35.236515	5
ACCAACG	25	0.0023532591	35.2	26
GCGCGAC	1185	0.0	35.088608	9
TAGGGTA	1825	0.0	34.9589	5
GGCGATA	1190	0.0	34.941177	8
TACGGGT	265	0.0	34.867924	4
>>END_MODULE