FastQCFastQC Report
Thu 26 May 2016
SRR1547235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547235_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1548882
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116850.7544151200672485No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19040.12292737600411136No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC16800.10846533176833355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG207.8573293E-444.0000041
TTCGCGA207.8573293E-444.00000432
TCGGGTA302.5282898E-644.05
TATAACG302.5282898E-644.01
CGTTTTT68100.041.6093981
GCGTAAG1050.039.809521
CTATGCG501.3478711E-939.61
GCGTTAG1000.039.61
TACACGG950.039.368422
GTATGCG452.3517714E-839.111111
CGGTCTA2400.038.531
CTACGGG3950.037.8734173
CGTTAGG2150.037.8604662
AGGGCGA17700.036.9152576
CGACGTC601.9826984E-1036.66666418
ATTCGAA301.3013043E-436.66666411
TCGATAG1200.036.6666641
TACGATA301.3013043E-436.66666432
TAGCATA7650.036.52287730
CGTTCGA1150.036.34782814
Produced by FastQC (version 0.11.5)