##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547235_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1548882
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38140542662385	31.0	31.0	33.0	30.0	34.0
2	31.77365480391663	31.0	31.0	34.0	30.0	34.0
3	31.901855661051002	31.0	31.0	34.0	30.0	34.0
4	35.58750505203108	37.0	35.0	37.0	33.0	37.0
5	35.47645398422863	37.0	35.0	37.0	33.0	37.0
6	35.51104086689625	37.0	35.0	37.0	33.0	37.0
7	35.990174203070346	37.0	35.0	37.0	35.0	37.0
8	35.983253081900365	37.0	35.0	37.0	35.0	37.0
9	37.68618848950404	39.0	37.0	39.0	35.0	39.0
10	37.04821671373287	39.0	37.0	39.0	33.0	39.0
11	36.83045771078752	39.0	37.0	39.0	32.0	39.0
12	36.355748856271816	38.0	35.0	39.0	32.0	39.0
13	36.19843474196227	39.0	35.0	39.0	32.0	39.0
14	37.24777613788526	39.0	35.0	41.0	32.0	41.0
15	37.419365710234864	39.0	35.0	41.0	32.0	41.0
16	37.47118502248719	39.0	35.0	41.0	32.0	41.0
17	37.397661022595656	39.0	35.0	41.0	32.0	41.0
18	37.331634688762605	39.0	35.0	41.0	32.0	41.0
19	37.298598602088475	39.0	35.0	41.0	32.0	41.0
20	37.12978135196871	39.0	35.0	41.0	32.0	41.0
21	37.0189446323219	39.0	35.0	41.0	32.0	41.0
22	36.94833886635651	39.0	35.0	41.0	31.0	41.0
23	36.84458596587732	38.0	35.0	41.0	31.0	41.0
24	36.78381568124622	38.0	35.0	41.0	31.0	41.0
25	36.599152808283655	38.0	35.0	41.0	31.0	41.0
26	36.484213774838885	38.0	35.0	40.0	31.0	41.0
27	36.40612777474333	38.0	35.0	40.0	30.0	41.0
28	36.46144509394518	38.0	35.0	40.0	31.0	41.0
29	36.37520676203869	38.0	35.0	40.0	30.0	41.0
30	36.35323607608585	38.0	35.0	40.0	30.0	41.0
31	36.229297002612206	38.0	35.0	40.0	30.0	41.0
32	35.99252299400471	38.0	35.0	40.0	30.0	41.0
33	35.90087237116837	38.0	35.0	40.0	30.0	41.0
34	35.73994984769659	38.0	35.0	40.0	29.0	41.0
35	35.547460038918395	38.0	35.0	40.0	28.0	41.0
36	35.38164172609663	38.0	34.0	40.0	27.0	41.0
37	35.368818928749896	38.0	34.0	40.0	27.0	41.0
38	35.31933032987665	38.0	34.0	40.0	27.0	41.0
39	35.22466785720281	38.0	34.0	40.0	27.0	41.0
40	35.11217445873863	37.0	34.0	40.0	26.0	41.0
41	35.102380297530736	37.0	34.0	40.0	26.0	41.0
42	34.901048627332486	37.0	34.0	40.0	26.0	41.0
43	34.89517148498078	37.0	34.0	40.0	26.0	41.0
44	34.61466787011535	37.0	34.0	40.0	24.0	41.0
45	34.50409908566308	36.0	34.0	40.0	24.0	41.0
46	34.48315300971927	36.0	34.0	40.0	24.0	41.0
47	34.36779819250272	36.0	33.0	40.0	24.0	41.0
48	34.30401218427227	36.0	33.0	40.0	24.0	41.0
49	34.27732067387961	36.0	33.0	40.0	24.0	41.0
50	33.772710251652484	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	5.0
13	9.0
14	21.0
15	33.0
16	123.0
17	279.0
18	614.0
19	1272.0
20	2227.0
21	3584.0
22	5614.0
23	8239.0
24	11421.0
25	16290.0
26	22031.0
27	27043.0
28	30529.0
29	33492.0
30	38437.0
31	46914.0
32	57560.0
33	73574.0
34	118294.0
35	177714.0
36	123472.0
37	154908.0
38	224672.0
39	370505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.78592946396174	23.06650861718323	27.182316018909127	14.965245899945897
2	34.06695926481165	25.054071259140464	27.94312284602701	12.935846630020881
3	29.65642314908431	25.738564977835626	30.76561029181048	13.839401581269586
4	25.910366315832967	27.5064207602645	31.342542556502046	15.240670367400485
5	23.343805402864774	31.699832524362737	29.5537038973918	15.402658175380694
6	20.209673816339784	41.151617747510784	27.51687991725645	11.12182851889298
7	86.22283685910224	4.052342270101918	7.750170768334838	1.9746501024610008
8	86.06730532087015	3.584456401456018	7.4268407793492335	2.9213974983245983
9	80.50219448608739	5.548453658832629	10.013545253931545	3.9358066011484416
10	41.18906411204985	28.764941422264574	15.905214212573973	14.140780253111599
11	36.01178140103636	24.815253841157688	23.590628595335218	15.582336162470737
12	32.605001543048466	21.944344372263348	27.46748945368337	17.983164631004815
13	24.348852914553852	29.350977027300985	28.09103598595632	18.209134072188842
14	20.163769738430688	32.50183035247359	28.3264315809726	19.007968328123123
15	18.56623035195709	23.57003309483873	38.88430493736772	18.979431615836457
16	20.58839859976422	20.75535773545047	37.56335214690338	21.09289151788193
17	21.11419720805071	21.297555268897177	29.36079055731812	28.227456965733992
18	24.054963515619654	23.275820882417126	30.944771777320675	21.72444382464255
19	27.902706597403803	26.324729708266997	25.312580299854993	20.459983394474207
20	29.756301642087646	24.354663557327154	24.85760697070532	21.031427829879874
21	25.351447043738645	27.709018504960355	26.29128623097176	20.648248220329243
22	24.42878153403552	24.01093175593751	26.27947125733271	25.280815452694267
23	22.46562359172616	28.580227544771002	25.33440249160362	23.619746371899215
24	22.449095541170987	25.118311143134207	31.5351976457858	20.897395669909006
25	23.1005331587558	25.59465472515014	28.770493814247956	22.534318301846106
26	20.91205140223723	29.099892696796786	26.802687357719957	23.18536854324603
27	22.14416592096751	27.14635459641212	27.580990675855226	23.128488806765137
28	20.73004915803786	26.755427463163755	31.948850848547533	20.565672530250854
29	21.735161232424417	25.178289889094195	30.851284991367965	22.235263887113415
30	23.435936372170378	25.83876628432637	29.8770338863774	20.84826345712585
31	27.966107166330296	25.164473471833233	24.834428962309588	22.034990399526887
32	28.474473846296878	25.157113324320378	25.741986800802124	20.626426028580617
33	27.083147715578075	25.63616853963052	25.00235653845806	22.278327206333344
34	23.332958869687943	25.987066800440576	28.414430537639408	22.265543792232073
35	23.575391798729665	25.21431587428868	29.29700261220674	21.913289714774915
36	27.11200724135215	25.370751290285508	27.119431951562483	20.39780951679986
37	24.262726276113998	27.35553773625105	27.621019548293546	20.760716439341408
38	23.238374517878057	28.206796902540027	27.054288189803998	21.500540389777917
39	23.21571301106217	26.482262690121004	26.515577042021278	23.786447256795544
40	24.315409437258616	24.595869795116737	28.18878390994278	22.899936857681862
41	21.248681306903947	24.275703378307707	28.132097861554335	26.34351745323401
42	23.730148584591984	26.591309086166664	25.137550826983592	24.540991502257757
43	23.140045529614266	25.415622365034906	26.687701193506026	24.756630911844802
44	22.892705835563977	26.629659328470474	27.863452477335265	22.614182358630288
45	21.319764836830696	28.30467395192145	26.74813187834838	23.62742933289947
46	24.25788407380291	27.168886977833047	26.950729623044232	21.62249932531981
47	23.430577668279444	26.018056895231528	27.60462062313333	22.9467448133557
48	23.236566762348584	24.967234431028317	28.245599083726198	23.550599722896905
49	22.83188777453673	24.06426054405694	28.576805721804497	24.52704595960183
50	21.74032624822291	26.540175429761597	28.782825289466857	22.936673032548637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1637.0
1	1821.0
2	2005.0
3	10441.0
4	18877.0
5	12910.5
6	6944.0
7	7014.5
8	7085.0
9	7327.0
10	7569.0
11	7712.0
12	7855.0
13	7583.5
14	7312.0
15	7037.0
16	6762.0
17	6322.5
18	5883.0
19	5957.0
20	6031.0
21	5891.5
22	5752.0
23	6729.5
24	7707.0
25	8074.5
26	8442.0
27	9929.0
28	11416.0
29	13868.0
30	16320.0
31	18688.0
32	21056.0
33	24175.0
34	27294.0
35	32345.0
36	37396.0
37	40609.0
38	43822.0
39	50430.5
40	57039.0
41	70679.5
42	84320.0
43	94575.5
44	104831.0
45	112941.5
46	121052.0
47	126771.5
48	132491.0
49	135608.5
50	138726.0
51	127477.0
52	116228.0
53	103105.0
54	89982.0
55	84161.5
56	78341.0
57	75902.5
58	73464.0
59	72611.0
60	71758.0
61	66069.0
62	60380.0
63	55474.5
64	50569.0
65	42933.5
66	35298.0
67	29898.5
68	24499.0
69	21623.0
70	18747.0
71	16249.5
72	13752.0
73	11358.0
74	8964.0
75	7193.0
76	5422.0
77	4248.5
78	3075.0
79	2393.5
80	1712.0
81	1120.0
82	528.0
83	396.0
84	264.0
85	235.5
86	207.0
87	124.5
88	42.0
89	28.0
90	14.0
91	9.0
92	4.0
93	5.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1548882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.31073964937187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31818648857481	20.342986748624742
2	6.71637990700062	3.534258462387316
3	2.669512675278712	2.107105589698692
4	1.529202779725366	1.6093782483379948
5	1.092469687776647	1.4371842764960971
6	0.8666616912716378	1.368150607387942
7	0.6747371478924985	1.2426983400971476
8	0.5841950846265616	1.2296483820841781
9	0.5229454726786158	1.2383173964218295
>10	6.864345478965263	42.971881936626126
>50	1.0856354968187178	18.548623237187115
>100	0.0717941627795162	2.7935223329920853
>500	0.002950444958329791	0.5052314327076138
>1k	7.376112395824477E-4	0.31510656961828254
>5k	0.0	0.0
>10k+	2.458704131941492E-4	0.7559064393327103
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11685	0.7544151200672485	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1904	0.12292737600411136	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1680	0.10846533176833355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047518145346127076	0.0
2	0.0	0.0	0.0	0.19685166462002915	0.0
3	0.0	0.0	0.0	0.33746921973397587	0.0
4	0.0	0.0	0.0	0.47453582648645926	0.0
5	0.0	0.0	0.0	0.7529947407226631	0.0
6	0.0	0.0	0.0	1.3414191655658727	0.0
7	0.0	0.0	0.0	1.7020018309981007	0.0
8	0.0	0.0	0.0	2.8026021349592805	0.0
9	0.0	0.0	0.0	3.513050058041865	0.0
10	0.0	0.0	0.0	4.5087359785961745	0.0
11	0.0	0.0	0.0	5.036600593202064	0.0
12	0.0	0.0	0.0	5.5618826999087085	0.0
13	0.0	0.0	0.0	5.870944332750978	0.0
14	0.0	0.0	0.0	6.02531374242841	0.0
15	0.0	0.0	0.0	6.15547214055041	0.0
16	0.0	0.0	0.0	6.362201897885055	0.0
17	0.0	0.0	0.0	6.574742297993004	0.0
18	0.0	0.0	0.0	6.93409827217309	0.0
19	0.0	0.0	0.0	7.075296891564367	0.0
20	0.0	0.0	0.0	7.258719515108317	0.0
21	0.0	0.0	0.0	7.421998577038147	0.0
22	0.0	0.0	0.0	7.58928052621181	0.0
23	0.0	0.0	0.0	7.781548239310677	0.0
24	0.0	0.0	0.0	7.939274909257128	0.0
25	6.456269748115092E-5	0.0	0.0	8.068464866916912	0.0
26	6.456269748115092E-5	0.0	0.0	8.205402348274433	0.0
27	6.456269748115092E-5	0.0	0.0	8.385209460759437	0.0
28	1.2912539496230183E-4	0.0	0.0	8.52828039837767	0.0
29	1.2912539496230183E-4	0.0	0.0	8.6713513359959	0.0
30	1.2912539496230183E-4	0.0	0.0	8.881438353599565	0.0
31	1.9368809244345276E-4	0.0	0.0	9.03148206254576	0.0
32	1.9368809244345276E-4	0.0	0.0	9.210320734568548	0.0
33	1.9368809244345276E-4	0.0	0.0	9.365077520430866	0.0
34	1.9368809244345276E-4	0.0	0.0	9.520544495965478	0.0
35	1.9368809244345276E-4	0.0	0.0	9.687761882441658	0.0
36	1.9368809244345276E-4	0.0	0.0	9.842195854816572	0.0
37	1.9368809244345276E-4	0.0	0.0	10.00934867859527	0.0
38	1.9368809244345276E-4	0.0	0.0	10.190898983912268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	20	7.8573293E-4	44.000004	1
TTCGCGA	20	7.8573293E-4	44.000004	32
TCGGGTA	30	2.5282898E-6	44.0	5
TATAACG	30	2.5282898E-6	44.0	1
CGTTTTT	6810	0.0	41.609398	1
GCGTAAG	105	0.0	39.80952	1
CTATGCG	50	1.3478711E-9	39.6	1
GCGTTAG	100	0.0	39.6	1
TACACGG	95	0.0	39.36842	2
GTATGCG	45	2.3517714E-8	39.11111	1
CGGTCTA	240	0.0	38.5	31
CTACGGG	395	0.0	37.873417	3
CGTTAGG	215	0.0	37.860466	2
AGGGCGA	1770	0.0	36.915257	6
CGACGTC	60	1.9826984E-10	36.666664	18
ATTCGAA	30	1.3013043E-4	36.666664	11
TCGATAG	120	0.0	36.666664	1
TACGATA	30	1.3013043E-4	36.666664	32
TAGCATA	765	0.0	36.522877	30
CGTTCGA	115	0.0	36.347828	14
>>END_MODULE