FastQCFastQC Report
Thu 26 May 2016
SRR1547234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547234_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1403545
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248861.773081732327784No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC24610.17534172399174947No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC18080.12881667491957866No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA14210.10124363664862901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT141100.042.955351
CGCATCG1000.041.80000321
CGTTTAT1050.039.80952539
GTTTACG501.3478711E-939.6000021
CGGTCTA3150.039.1111131
GTTTTTT160850.038.3786132
TCACGAC3250.037.90769225
TATACTA13350.037.73782744
TAAGGGA12800.037.296884
TAGCCGT950.037.0526344
TACGGGA4300.036.8372084
CGGTAAT301.3012422E-436.6666646
TAGGGAC11900.036.4201665
CGAGGGT1450.036.413794
ATGGGAC8900.036.337085
CATATGC7300.036.16438333
GGCGATA5050.036.1584178
ACGTTAG553.425157E-936.01
GTACGAG1650.036.01
ATTGGGA10400.035.9615364
Produced by FastQC (version 0.11.5)