##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547234_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1403545
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43785129796337	31.0	31.0	33.0	30.0	34.0
2	31.841574014370753	31.0	31.0	34.0	30.0	34.0
3	31.926970635070482	33.0	31.0	34.0	30.0	34.0
4	35.646705306919266	37.0	35.0	37.0	33.0	37.0
5	35.56265385149746	37.0	35.0	37.0	33.0	37.0
6	35.59605427684898	37.0	35.0	37.0	33.0	37.0
7	36.00971397425804	37.0	35.0	37.0	35.0	37.0
8	35.99644257932592	37.0	35.0	37.0	35.0	37.0
9	37.718240597914566	39.0	38.0	39.0	35.0	39.0
10	37.144568218332864	39.0	37.0	39.0	33.0	39.0
11	36.966872455104756	39.0	37.0	39.0	33.0	39.0
12	36.74741030747144	39.0	35.0	39.0	32.0	39.0
13	36.73102465542608	39.0	35.0	39.0	32.0	39.0
14	37.88320787719667	40.0	37.0	41.0	33.0	41.0
15	37.94775799849666	40.0	37.0	41.0	33.0	41.0
16	37.96585431888539	40.0	36.0	41.0	33.0	41.0
17	37.874246283517806	40.0	36.0	41.0	33.0	41.0
18	37.71984866890623	39.0	36.0	41.0	33.0	41.0
19	37.57596942028934	39.0	36.0	41.0	33.0	41.0
20	37.32585702631551	39.0	35.0	41.0	32.0	41.0
21	37.21431161808136	39.0	35.0	41.0	32.0	41.0
22	37.1610073064989	39.0	35.0	41.0	32.0	41.0
23	37.05065459247833	39.0	35.0	41.0	32.0	41.0
24	36.95476454264024	39.0	35.0	41.0	32.0	41.0
25	36.76907402327677	38.0	35.0	41.0	31.0	41.0
26	36.67522808317511	38.0	35.0	41.0	31.0	41.0
27	36.56721658372193	38.0	35.0	40.0	31.0	41.0
28	36.58932203812489	38.0	35.0	40.0	31.0	41.0
29	36.46419245553224	38.0	35.0	40.0	31.0	41.0
30	36.3639690925478	38.0	35.0	40.0	31.0	41.0
31	36.17029236682828	38.0	35.0	40.0	30.0	41.0
32	35.7790851023658	38.0	35.0	40.0	28.0	41.0
33	35.486554403314464	38.0	35.0	40.0	26.0	41.0
34	35.14476913814662	38.0	34.0	40.0	24.0	41.0
35	34.79798153960151	38.0	34.0	40.0	23.0	41.0
36	34.60485413720259	38.0	34.0	40.0	22.0	41.0
37	34.50528198240883	38.0	34.0	40.0	21.0	41.0
38	34.40431122621647	38.0	34.0	40.0	21.0	41.0
39	34.24387817989448	37.0	33.0	40.0	21.0	41.0
40	34.120356668293496	37.0	33.0	40.0	20.0	41.0
41	34.05499289299595	37.0	33.0	40.0	20.0	41.0
42	33.85656177749912	37.0	33.0	40.0	18.0	41.0
43	33.867305287682264	37.0	33.0	40.0	18.0	41.0
44	33.60840799546862	36.0	33.0	40.0	18.0	41.0
45	33.497207428333255	36.0	33.0	40.0	18.0	41.0
46	33.471473305095316	36.0	33.0	40.0	19.0	41.0
47	33.35101902682137	36.0	33.0	40.0	18.0	41.0
48	33.29899718213524	35.0	33.0	40.0	19.0	41.0
49	33.24044544350199	35.0	33.0	40.0	19.0	41.0
50	32.76115479019198	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	5.0
13	5.0
14	17.0
15	39.0
16	98.0
17	239.0
18	607.0
19	1190.0
20	2184.0
21	3610.0
22	5440.0
23	8017.0
24	11986.0
25	19077.0
26	28711.0
27	37015.0
28	37194.0
29	34302.0
30	35486.0
31	40226.0
32	48471.0
33	61235.0
34	91823.0
35	125087.0
36	122806.0
37	154864.0
38	215822.0
39	317982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.09344908784542	21.34701773010484	25.173257715285224	21.386275466764516
2	38.38886533741348	23.192202601270353	25.83814555286792	12.580786508448249
3	27.86002586308241	24.026803558133157	35.307596122675086	12.805574456109351
4	24.46298479920487	26.054811210185637	34.96453622790862	14.517667762700876
5	21.659440915681362	29.605748301621965	33.88890274269795	14.845908039998717
6	19.090089737058662	38.19670904744771	31.908203869487618	10.804997346006006
7	80.9896369549961	3.54815841316096	13.40413025588777	2.058074375955171
8	80.73862968412128	3.1481712378299234	13.348271697736802	2.764927380311996
9	75.77619527695941	4.900519755333815	15.486500254712176	3.836784712994596
10	35.79450605431248	28.890630510599948	21.250191479432438	14.064671955655145
11	28.673181123512247	24.244894178669014	29.460402053371997	17.621522644446742
12	25.370187632031747	20.24858483340399	34.51667028844818	19.864557246116085
13	23.219348150575865	23.16313335162036	34.35265702204062	19.264861475763155
14	20.625416356440297	25.615281305551303	34.021281825662875	19.738020512345525
15	18.31783092098935	22.06804911848213	38.299520143636286	21.314599816892226
16	20.852056756284977	20.75010063802728	36.482549544189894	21.915293061497852
17	20.834672205023708	21.129639591178055	33.7166246896252	24.31906351417304
18	23.267013170222544	20.898011820069897	34.05441222048456	21.780562789223005
19	25.40061059673897	24.390026682436257	29.347259973851926	20.862102746972845
20	27.25897637767225	21.76602816439801	29.319615687420068	21.655379770509672
21	25.0928897897823	24.166521201671483	30.412134986765654	20.328454021780562
22	24.254085191425997	20.6722263981561	29.93954593547054	25.134142474947364
23	22.214606585467514	25.38393852708677	29.56314190139967	22.83831298604605
24	21.607358510058457	23.472136625473354	33.570993448731606	21.34951141573658
25	23.43152517375645	23.861579072990178	30.533755597433643	22.17314015581973
26	21.364259785044297	24.012126436986346	30.412348731248372	24.211265046720982
27	22.112864211692536	22.434335913704228	31.53279730966944	23.920002564933792
28	21.751849780377544	23.758839224962504	32.789187379100774	21.70012361555917
29	22.22116141627094	22.584598285056767	31.65669786148645	23.53754243718584
30	23.284825210449256	21.976210239073204	33.13003858087913	21.60892596959841
31	25.873840881482245	22.339575859698122	28.92112472346808	22.865458535351557
32	25.11839663138695	23.038092829228844	29.494458674285468	22.349051865098733
33	24.63896775664478	23.12095444036351	27.605883673127686	24.634194129864024
34	23.283898984357464	24.179132126151995	29.58145267875273	22.955516210737812
35	24.254655176713253	23.392552429740405	29.205333637325488	23.147458756220853
36	27.622199501975352	25.012949353244817	25.602527884748977	21.76232326003085
37	24.468684652077417	26.35562094553434	26.635982458702784	22.539711943685454
38	24.933650150155497	26.29164009704	27.280849563070653	21.49386018973385
39	24.39700900220513	25.65012165623475	25.89322038124891	24.05964896031121
40	25.815274893216817	24.06940995835545	29.076374466084093	21.038940682343636
41	23.037807836585216	24.260212533263985	28.966937290931178	23.73504233921962
42	24.013978889169923	26.288576426121	25.876904552401243	23.820540132307837
43	23.23345528643542	24.849221079480884	27.82611173849075	24.091211895592945
44	24.293841665212014	24.582681709528373	29.136294169406753	21.987182455852857
45	23.056189862099185	24.795642462478938	28.135471253148275	24.0126964222736
46	24.424867033119707	24.76194208237	27.959773288351997	22.853417596158298
47	23.359422034918722	26.047828890416767	28.443477052748577	22.149272021915934
48	23.7192252474983	24.628565525152382	28.348645750581564	23.303563476767756
49	23.644842167511552	24.798207396271586	28.330976206676667	23.2259742295402
50	22.20206690914791	24.58731283998732	30.597807694088896	22.61281255677588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1014.0
1	1433.0
2	1852.0
3	20247.0
4	38642.0
5	26321.5
6	14001.0
7	14047.5
8	14094.0
9	14321.0
10	14548.0
11	14288.0
12	14028.0
13	13555.0
14	13082.0
15	12284.5
16	11487.0
17	10673.5
18	9860.0
19	9272.0
20	8684.0
21	8245.5
22	7807.0
23	7666.0
24	7525.0
25	7932.5
26	8340.0
27	9240.0
28	10140.0
29	11706.0
30	13272.0
31	15254.5
32	17237.0
33	19553.5
34	21870.0
35	25732.5
36	29595.0
37	32198.5
38	34802.0
39	38427.0
40	42052.0
41	46282.0
42	50512.0
43	55694.5
44	60877.0
45	64613.0
46	68349.0
47	77356.0
48	86363.0
49	99610.0
50	112857.0
51	111814.5
52	110772.0
53	101416.0
54	92060.0
55	84997.5
56	77935.0
57	76811.5
58	75688.0
59	74105.0
60	72522.0
61	68681.5
62	64841.0
63	60420.0
64	55999.0
65	48938.5
66	41878.0
67	35998.0
68	30118.0
69	27213.0
70	24308.0
71	21436.0
72	18564.0
73	15306.5
74	12049.0
75	9603.5
76	7158.0
77	5369.0
78	3580.0
79	2715.5
80	1851.0
81	1344.0
82	837.0
83	561.0
84	285.0
85	214.0
86	143.0
87	90.0
88	37.0
89	27.0
90	17.0
91	11.5
92	6.0
93	4.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1403545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.75262876539435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.66192228517541	25.621021040764163
2	7.929228123381986	5.669814989828013
3	4.034691799963825	4.32752514320662
4	2.753023637528334	3.937113283796244
5	2.0959734050143477	3.746827952580875
6	1.7003795983876426	3.6475824324842234
7	1.4040690749969693	3.513941227953609
8	1.1626512954760213	3.32542721206072
9	0.9627823689005287	3.09798005554508
>10	6.183177134793453	35.95992310603419
>50	0.07336958490450193	1.7298014535387392
>100	0.03413979644149629	2.289492196312758
>500	0.0037933045945015157	0.9510573791831897
>1k	5.989428307107657E-4	0.40614804019456235
>5k	0.0	0.0
>10k+	1.996476102369219E-4	1.7763444865170261
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24886	1.773081732327784	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2461	0.17534172399174947	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	1808	0.12881667491957866	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1421	0.10124363664862901	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039756473786020395	0.0
2	0.0	0.0	0.0	0.1527560569842791	0.0
3	0.0	0.0	0.0	0.34669355097271554	0.0
4	0.0	0.0	0.0	0.47258905129511347	0.0
5	0.0	0.0	0.0	0.7191076880328027	0.0
6	0.0	0.0	0.0	1.338040461830579	0.0
7	0.0	0.0	0.0	1.7222817936012027	0.0
8	0.0	0.0	0.0	2.7528864411187386	0.0
9	0.0	0.0	0.0	3.4296727215728744	0.0
10	0.0	0.0	0.0	4.289068038431258	0.0
11	0.0	0.0	0.0	4.718409456055916	0.0
12	0.0	0.0	0.0	5.162926731953731	0.0
13	0.0	0.0	0.0	5.464163956267879	0.0
14	0.0	0.0	0.0	5.642640599339529	0.0
15	0.0	0.0	0.0	5.763406232076634	0.0
16	0.0	0.0	0.0	5.915022318486404	0.0
17	0.0	0.0	0.0	6.059869829610023	0.0
18	0.0	0.0	0.0	6.315365734621975	0.0
19	7.12481609068466E-5	0.0	0.0	6.429434040233836	0.0
20	7.12481609068466E-5	0.0	0.0	6.5764902443455675	0.0
21	7.12481609068466E-5	0.0	0.0	6.704950678460612	0.0
22	7.12481609068466E-5	0.0	0.0	6.843029614298081	0.0
23	7.12481609068466E-5	0.0	0.0	6.984955950824519	0.0
24	7.12481609068466E-5	0.0	0.0	7.1114926845950786	0.0
25	7.12481609068466E-5	0.0	0.0	7.22285356009248	0.0
26	7.12481609068466E-5	0.0	0.0	7.335568150647111	0.0
27	7.12481609068466E-5	7.12481609068466E-5	0.0	7.500721387629182	0.0
28	7.12481609068466E-5	7.12481609068466E-5	0.0	7.625833158181605	0.0
29	7.12481609068466E-5	7.12481609068466E-5	0.0	7.74681253540143	0.0
30	7.12481609068466E-5	7.12481609068466E-5	0.0	7.9243629523812915	0.0
31	7.12481609068466E-5	7.12481609068466E-5	0.0	8.053892108909938	0.0
32	7.12481609068466E-5	7.12481609068466E-5	0.0	8.218617856926569	0.0
33	7.12481609068466E-5	7.12481609068466E-5	0.0	8.352920640235974	0.0
34	7.12481609068466E-5	7.12481609068466E-5	0.0	8.478388651592931	0.0
35	7.12481609068466E-5	7.12481609068466E-5	0.0	8.62031498811937	0.0
36	7.12481609068466E-5	7.12481609068466E-5	0.0	8.74884667039532	0.0
37	7.12481609068466E-5	7.12481609068466E-5	0.0	8.88307820554382	0.0
38	7.12481609068466E-5	7.12481609068466E-5	0.0	9.028210709311066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14110	0.0	42.95535	1
CGCATCG	100	0.0	41.800003	21
CGTTTAT	105	0.0	39.809525	39
GTTTACG	50	1.3478711E-9	39.600002	1
CGGTCTA	315	0.0	39.11111	31
GTTTTTT	16085	0.0	38.378613	2
TCACGAC	325	0.0	37.907692	25
TATACTA	1335	0.0	37.737827	44
TAAGGGA	1280	0.0	37.29688	4
TAGCCGT	95	0.0	37.05263	44
TACGGGA	430	0.0	36.837208	4
CGGTAAT	30	1.3012422E-4	36.666664	6
TAGGGAC	1190	0.0	36.420166	5
CGAGGGT	145	0.0	36.41379	4
ATGGGAC	890	0.0	36.33708	5
CATATGC	730	0.0	36.164383	33
GGCGATA	505	0.0	36.158417	8
ACGTTAG	55	3.425157E-9	36.0	1
GTACGAG	165	0.0	36.0	1
ATTGGGA	1040	0.0	35.961536	4
>>END_MODULE