FastQCFastQC Report
Thu 26 May 2016
SRR1547233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547233_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1774701
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310201.747900068800322No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC26950.15185656626102087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT179950.042.924151
ACGGGTA1350.040.740745
TAATGCG501.3496901E-939.61
GTTTTTT205900.038.1340452
AGGGCGA20150.037.339956
CGGTCTA1550.036.90322531
TACGGGA5500.036.44
CGCCGTT1700.036.2352926
TATACTA7000.036.1428644
GCGATAT2200.036.09
TGGGCGA12900.035.8139536
ATAGGGC11050.035.438914
GTAGGGA18450.035.414634
GGCGATA8700.035.4022988
GGGCGAT42150.035.335717
GTACGGG4300.035.3023263
TAGGGAC12500.035.25
AGTACGG1500.035.22
ACGGGAC3450.035.0724645
GTTGATC11700.034.9743616
Produced by FastQC (version 0.11.5)