##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547233_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1774701
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31100281117777	31.0	31.0	33.0	30.0	34.0
2	31.708139004823913	31.0	31.0	34.0	30.0	34.0
3	31.788368294152086	31.0	31.0	34.0	30.0	34.0
4	35.51929648994394	37.0	35.0	37.0	33.0	37.0
5	35.42260752656363	37.0	35.0	37.0	33.0	37.0
6	35.44893590525953	37.0	35.0	37.0	33.0	37.0
7	35.93129941325328	37.0	35.0	37.0	35.0	37.0
8	35.91064410286578	37.0	35.0	37.0	35.0	37.0
9	37.61178981698889	39.0	37.0	39.0	35.0	39.0
10	37.007548877247494	39.0	37.0	39.0	33.0	39.0
11	36.81276057206256	39.0	37.0	39.0	32.0	39.0
12	36.579113890170795	39.0	35.0	39.0	32.0	39.0
13	36.54470245973829	39.0	35.0	39.0	32.0	39.0
14	37.635079937409174	40.0	36.0	41.0	32.0	41.0
15	37.7218100401138	40.0	36.0	41.0	32.0	41.0
16	37.7120827677451	40.0	36.0	41.0	32.0	41.0
17	37.587382888723226	40.0	36.0	41.0	32.0	41.0
18	37.44359134299243	39.0	36.0	41.0	32.0	41.0
19	37.31068670159086	39.0	36.0	41.0	32.0	41.0
20	37.03829152065616	39.0	35.0	41.0	32.0	41.0
21	36.95237394918919	39.0	35.0	41.0	31.0	41.0
22	36.87463860109393	38.0	35.0	41.0	31.0	41.0
23	36.7239923795614	38.0	35.0	41.0	31.0	41.0
24	36.65782517731156	38.0	35.0	41.0	31.0	41.0
25	36.47823154435592	38.0	35.0	40.0	31.0	41.0
26	36.35891567086512	38.0	35.0	40.0	30.0	41.0
27	36.25778370553687	38.0	35.0	40.0	30.0	41.0
28	36.27489926472121	38.0	35.0	40.0	30.0	41.0
29	36.122216643817744	38.0	35.0	40.0	30.0	41.0
30	36.03793822170608	38.0	35.0	40.0	30.0	41.0
31	35.85028407602182	38.0	35.0	40.0	29.0	41.0
32	35.43438303128245	38.0	34.0	40.0	26.0	41.0
33	35.154241756780436	38.0	34.0	40.0	24.0	41.0
34	34.819103048907955	38.0	34.0	40.0	23.0	41.0
35	34.47671523259411	38.0	34.0	40.0	21.0	41.0
36	34.31683027169084	38.0	33.0	40.0	21.0	41.0
37	34.24653730403037	38.0	33.0	40.0	20.0	41.0
38	34.15597895082045	38.0	33.0	40.0	20.0	41.0
39	33.99767566480213	37.0	33.0	40.0	18.0	41.0
40	33.88939939741962	37.0	33.0	40.0	18.0	41.0
41	33.83589517332779	37.0	33.0	40.0	18.0	41.0
42	33.62474692920103	37.0	33.0	40.0	18.0	41.0
43	33.632324543683694	37.0	33.0	40.0	18.0	41.0
44	33.368001144981605	36.0	33.0	40.0	17.0	41.0
45	33.26143164397834	36.0	32.0	40.0	18.0	41.0
46	33.239701786385424	36.0	32.0	40.0	18.0	41.0
47	33.11851517523233	36.0	32.0	40.0	18.0	41.0
48	33.069129954848734	35.0	32.0	40.0	18.0	41.0
49	33.030156065726004	35.0	32.0	40.0	18.0	41.0
50	32.52858087080584	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	7.0
13	8.0
14	29.0
15	54.0
16	169.0
17	383.0
18	993.0
19	1923.0
20	3530.0
21	5714.0
22	8412.0
23	12601.0
24	18349.0
25	26866.0
26	39533.0
27	48857.0
28	49301.0
29	47309.0
30	49376.0
31	56261.0
32	66050.0
33	81191.0
34	118848.0
35	156749.0
36	148724.0
37	182869.0
38	257098.0
39	393494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.7473749099144	21.37706577051571	24.060560060539775	20.814999259030113
2	39.52062910879072	22.888644340652313	25.00984672911099	12.58087982144598
3	28.380217287306426	23.930397289458902	34.951014283532835	12.738371139701844
4	24.42631181252504	26.037005670250934	35.2575447920523	14.279137725171735
5	21.990915652833916	29.486037366294376	33.845926722304206	14.6771202585675
6	18.613219917045182	38.75216163173402	32.252193468082794	10.382424983138003
7	79.67629476740026	3.55231670010892	14.57327177930254	2.1981167531882835
8	78.84184434448395	3.5852799992787516	14.128858889469267	3.4440167667680357
9	73.23081465553915	5.159066231438422	16.883069317028614	4.727049795993804
10	34.4960644074692	28.747715812410092	22.1188808706368	14.637338909483907
11	28.609213608376848	24.845537360941364	30.723879684521506	15.821369346160283
12	25.326632486261065	20.828184578698046	34.50902433705734	19.336158597983548
13	23.500522059772322	22.197316618405015	36.08264152665717	18.219519795165496
14	20.144914551803375	25.535456395189946	35.525758987006824	18.79387006599985
15	19.815732340264642	21.90633802539132	38.92295096469772	19.35497866964632
16	22.565265923668267	21.145364768487763	36.8819874446456	19.407381863198363
17	21.473420029627526	21.935075260565018	34.899230912700226	21.69227379710723
18	23.43059478751632	21.698246634221764	34.40703532595068	20.464123252311232
19	25.130655811880427	25.468064761331625	30.43932470878193	18.96195471800602
20	26.38754359185012	24.091945629151052	29.684155246433065	19.836355532565765
21	24.637671359851606	26.112173261862136	30.97327380781326	18.276881570472998
22	23.17804520310745	22.005735050580352	31.60487315891522	23.211346587396974
23	22.110541437684432	25.714190728466374	30.862494583594646	21.312773250254548
24	21.948373275272846	24.550276356411587	33.38224298064857	20.119107387666993
25	23.10744176061207	24.43634167107586	31.41994059844447	21.0362759698676
26	20.404451228685847	24.51134022012722	32.56723245211447	22.516976099072465
27	22.09589108249784	22.320266906932492	32.107042256695635	23.476799753874033
28	21.596088580555257	24.99728123216249	33.04212935023984	20.36450083704241
29	22.283302933846322	24.15905552540963	31.864635225877485	21.69300631486656
30	23.394081594589736	23.527174436707927	32.538213479341024	20.540530489361306
31	25.094255313993735	22.973785443294393	29.142261147089005	22.789698095622867
32	24.710979483304513	24.685679446847665	28.400840479607552	22.20250059024027
33	23.39351811939025	24.01762325033907	27.364496892716012	25.224361737554663
34	21.883179194692516	26.002408293002592	29.11870788375056	22.995704628554332
35	22.0021851568236	25.16874673536556	29.012830893767454	23.81623721404338
36	24.702921787951887	27.344944303293907	25.733912360448326	22.218221548305884
37	22.96877051401898	27.117074932622454	27.11279252110637	22.801362032252193
38	23.348552798471406	28.30566951841465	26.73408083953297	21.611696843580972
39	22.87974143250046	26.846550489350037	26.394474336803775	23.879233741345725
40	23.444061844783995	25.76456541130027	29.05221781021141	21.739154933704324
41	21.59890595655268	25.862102968331	28.75166013880648	23.787330936309832
42	23.09087558974723	27.09487400976277	27.05030312148356	22.763947279006437
43	22.32314063044986	25.169873685764532	28.541991017078374	23.964994666707238
44	23.327535173530638	25.90599768637083	28.81414953842929	21.95231760166924
45	22.259862365547775	25.122654464047745	29.297554912066875	23.3199282583376
46	24.268876841789126	25.260536845361557	28.07368677878696	22.39689953406236
47	22.581099576773777	25.698751508000502	29.104226571123814	22.615922344101907
48	22.935525477249406	25.27005957623284	28.546498818674245	23.247916127843506
49	23.319759215777754	24.311306524310293	28.67412595135744	23.69480830855451
50	21.808124298121204	23.812405582686885	30.91224944370911	23.4672206754828
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1334.0
1	1921.0
2	2508.0
3	26105.0
4	49702.0
5	34009.0
6	18316.0
7	18286.5
8	18257.0
9	18503.5
10	18750.0
11	18559.0
12	18368.0
13	17613.5
14	16859.0
15	16007.5
16	15156.0
17	14184.0
18	13212.0
19	12375.5
20	11539.0
21	11231.5
22	10924.0
23	10816.5
24	10709.0
25	11109.0
26	11509.0
27	12527.5
28	13546.0
29	15603.5
30	17661.0
31	19825.5
32	21990.0
33	24790.5
34	27591.0
35	32292.5
36	36994.0
37	40479.5
38	43965.0
39	49168.0
40	54371.0
41	62977.5
42	71584.0
43	80445.0
44	89306.0
45	101931.0
46	114556.0
47	130659.0
48	146762.0
49	151320.5
50	155879.0
51	141795.0
52	127711.0
53	115236.0
54	102761.0
55	97500.0
56	92239.0
57	89891.5
58	87544.0
59	84469.0
60	81394.0
61	76953.0
62	72512.0
63	65901.0
64	59290.0
65	50513.5
66	41737.0
67	35976.0
68	30215.0
69	26976.0
70	23737.0
71	21099.5
72	18462.0
73	14979.0
74	11496.0
75	9189.5
76	6883.0
77	5479.5
78	4076.0
79	2927.0
80	1778.0
81	1338.5
82	899.0
83	651.0
84	403.0
85	260.0
86	117.0
87	91.5
88	66.0
89	44.5
90	23.0
91	14.5
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1774701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.76431233755752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84754514993483	26.053423577665928
2	7.759297952755519	5.550119110050381
3	3.756041026904825	4.029966733167134
4	2.5379884487154376	3.63077646355888
5	1.9322606504609319	3.4552986710328417
6	1.5531024130570537	3.3327383875672045
7	1.2358660189490045	3.093992881634619
8	1.0859384143550443	3.1070272504276613
9	0.9016847779502176	2.9023322425769504
>10	6.263477555397769	37.29014585325138
>50	0.08858203468282615	2.101701364129305
>100	0.033934286876095385	2.3265154582114156
>500	0.0031713110818145864	0.8168491118770342
>1k	9.51393324544376E-4	0.5499724164803859
>5k	0.0	0.0
>10k+	1.5856555409072935E-4	1.7591404783688975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31020	1.747900068800322	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2695	0.15185656626102087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049585817554619056	0.0
2	0.0	0.0	0.0	0.1693242974450344	0.0
3	0.0	0.0	0.0	0.35420051039583567	0.0
4	0.0	0.0	0.0	0.5544032487726102	0.0
5	0.0	0.0	0.0	0.9872085494965067	0.0
6	0.0	0.0	0.0	1.9379602535863787	0.0
7	0.0	0.0	0.0	2.571757157966328	0.0
8	0.0	0.0	0.0	4.170167256343463	0.0
9	0.0	0.0	0.0	5.185662260854082	0.0
10	0.0	0.0	0.0	6.491177950539274	0.0
11	0.0	0.0	0.0	7.006757758067415	0.0
12	0.0	0.0	0.0	7.557554765563326	0.0
13	0.0	0.0	0.0	7.963707689351614	0.0
14	0.0	0.0	0.0	8.191295322423327	0.0
15	0.0	0.0	0.0	8.341348768045998	0.0
16	0.0	0.0	0.0	8.540030123384165	0.0
17	0.0	0.0	0.0	8.718370024020947	0.0
18	0.0	0.0	0.0	9.066710392342147	0.0
19	0.0	0.0	0.0	9.217214618124405	0.0
20	0.0	0.0	0.0	9.392004625004438	0.0
21	0.0	0.0	0.0	9.537437573991337	0.0
22	0.0	0.0	0.0	9.694647154647459	0.0
23	0.0	0.0	0.0	9.862506416573835	0.0
24	0.0	0.0	0.0	10.018363656751193	0.0
25	0.0	0.0	0.0	10.152527101748408	0.0
26	0.0	0.0	0.0	10.288550014903919	0.0
27	0.0	0.0	0.0	10.461029773466066	0.0
28	0.0	0.0	0.0	10.606237332373171	0.0
29	0.0	0.0	0.0	10.764404820868418	0.0
30	0.0	0.0	0.0	10.972101779398333	0.0
31	0.0	0.0	0.0	11.130832743093062	0.0
32	0.0	0.0	0.0	11.32765463027293	0.0
33	0.0	0.0	0.0	11.500923254114355	0.0
34	0.0	0.0	0.0	11.681178970429386	0.0
35	0.0	0.0	0.0	11.86306876482292	0.0
36	0.0	0.0	0.0	12.042930048498311	0.0
37	0.0	0.0	0.0	12.2210445590553	5.634751994843075E-5
38	0.0	0.0	0.0	12.432460453901813	5.634751994843075E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17995	0.0	42.92415	1
ACGGGTA	135	0.0	40.74074	5
TAATGCG	50	1.3496901E-9	39.6	1
GTTTTTT	20590	0.0	38.134045	2
AGGGCGA	2015	0.0	37.33995	6
CGGTCTA	155	0.0	36.903225	31
TACGGGA	550	0.0	36.4	4
CGCCGTT	170	0.0	36.23529	26
TATACTA	700	0.0	36.14286	44
GCGATAT	220	0.0	36.0	9
TGGGCGA	1290	0.0	35.813953	6
ATAGGGC	1105	0.0	35.43891	4
GTAGGGA	1845	0.0	35.41463	4
GGCGATA	870	0.0	35.402298	8
GGGCGAT	4215	0.0	35.33571	7
GTACGGG	430	0.0	35.302326	3
TAGGGAC	1250	0.0	35.2	5
AGTACGG	150	0.0	35.2	2
ACGGGAC	345	0.0	35.072464	5
GTTGATC	1170	0.0	34.97436	16
>>END_MODULE