FastQCFastQC Report
Thu 26 May 2016
SRR1547232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547232_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2861945
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142230.4969697181462257No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49850.17418224319475042No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG29010.10136463139578154TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA207.858449E-444.0000046
CTATCGT750.044.013
CGTTACA302.5290447E-644.038
CGACTCA254.444508E-544.018
TACGTAA254.444508E-544.029
GTACGAC254.444508E-544.036
CGGTCTA5250.041.48571431
CGTTTTT90600.041.426051
TTACGTC603.6379788E-1240.33333215
ACGGTCT5400.039.92592630
CGACGGT5600.039.28571328
TCTAGCG452.3530447E-839.111111
CGCATCG2150.038.88372421
GCGTAAG2750.038.41
ACGATTG750.038.133331
ATATCGA750.038.1333314
TACGGGA6500.037.9076924
ACGGGTA3250.037.9076925
GTATCGC357.294011E-637.71428731
TATAGCG1700.037.529411
Produced by FastQC (version 0.11.5)