##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547232_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2861945
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307463630503033	31.0	31.0	33.0	30.0	34.0
2	31.686127790715755	31.0	31.0	34.0	30.0	34.0
3	31.8347546860614	31.0	31.0	34.0	30.0	34.0
4	35.51460073481496	37.0	35.0	37.0	33.0	37.0
5	35.3887607903017	37.0	35.0	37.0	33.0	37.0
6	35.421616068792375	37.0	35.0	37.0	33.0	37.0
7	35.93624545545075	37.0	35.0	37.0	35.0	37.0
8	35.92544091518181	37.0	35.0	37.0	35.0	37.0
9	37.6170600762768	39.0	37.0	39.0	35.0	39.0
10	36.96294163584555	39.0	37.0	39.0	32.0	39.0
11	36.69999807823002	39.0	35.0	39.0	32.0	39.0
12	36.05442662245431	38.0	35.0	39.0	32.0	39.0
13	35.75806138832158	38.0	35.0	39.0	31.0	39.0
14	36.75338554724147	39.0	35.0	41.0	31.0	41.0
15	36.99791156014528	39.0	35.0	41.0	32.0	41.0
16	37.122457629339486	39.0	35.0	41.0	32.0	41.0
17	37.07716780021978	38.0	35.0	41.0	32.0	41.0
18	37.05002681742661	38.0	35.0	41.0	32.0	41.0
19	37.020966859950136	38.0	35.0	41.0	32.0	41.0
20	36.85083815377305	38.0	35.0	41.0	32.0	41.0
21	36.71817313051089	38.0	35.0	41.0	31.0	41.0
22	36.634920307692845	38.0	35.0	41.0	31.0	41.0
23	36.54648988712222	38.0	35.0	40.0	31.0	41.0
24	36.48754745461566	38.0	35.0	40.0	31.0	41.0
25	36.32928480456473	38.0	35.0	40.0	31.0	41.0
26	36.20735234255026	38.0	35.0	40.0	31.0	41.0
27	36.1108131008807	38.0	35.0	40.0	30.0	41.0
28	36.188162945129974	38.0	35.0	40.0	30.0	41.0
29	36.15362803967232	38.0	35.0	40.0	30.0	41.0
30	36.13145605523516	38.0	35.0	40.0	30.0	41.0
31	36.03572500519751	38.0	35.0	40.0	30.0	41.0
32	35.8196988411727	38.0	35.0	40.0	30.0	41.0
33	35.77410572180807	38.0	35.0	40.0	30.0	41.0
34	35.722860851623636	38.0	35.0	40.0	30.0	41.0
35	35.54614326969945	38.0	35.0	40.0	29.0	41.0
36	35.410689234069835	38.0	34.0	40.0	28.0	41.0
37	35.35878502207415	37.0	34.0	40.0	28.0	41.0
38	35.35551032601954	37.0	34.0	40.0	28.0	41.0
39	35.27478725132733	37.0	34.0	40.0	27.0	41.0
40	35.16040559829067	37.0	34.0	40.0	27.0	41.0
41	35.184474893822205	37.0	34.0	40.0	27.0	41.0
42	34.968200996175675	37.0	34.0	40.0	26.0	41.0
43	34.97931791142038	37.0	34.0	40.0	27.0	41.0
44	34.63005508491603	36.0	34.0	40.0	26.0	41.0
45	34.55979412602269	36.0	34.0	40.0	25.0	41.0
46	34.57924348651005	36.0	34.0	40.0	26.0	41.0
47	34.48400510841403	36.0	33.0	40.0	25.0	41.0
48	34.49150525254678	36.0	34.0	40.0	26.0	41.0
49	34.51754348878123	36.0	34.0	40.0	26.0	41.0
50	33.95161227766432	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	6.0
13	11.0
14	24.0
15	62.0
16	213.0
17	538.0
18	1145.0
19	2353.0
20	4235.0
21	7006.0
22	10205.0
23	14646.0
24	20986.0
25	28886.0
26	38869.0
27	47807.0
28	54842.0
29	62417.0
30	74779.0
31	91147.0
32	114266.0
33	146054.0
34	242977.0
35	396010.0
36	210997.0
37	257288.0
38	373390.0
39	660781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.80264121078497	24.19955659525253	28.5132313863474	13.484570807615102
2	30.551635338904138	26.167658707627155	29.625516912449402	13.655189041019305
3	29.278969372227632	25.489553433067375	31.7499462777936	13.481530916911403
4	25.636132071021628	28.214029270303936	30.75922143856713	15.390617220107305
5	22.827762238617446	31.921298277919384	29.976082699003648	15.27485678445952
6	19.923303906958377	41.50551460632542	27.145315510954966	11.425865975761239
7	85.71268839897343	4.40287985967585	7.363069520902743	2.521362220447982
8	85.26128210011024	4.065731521744827	6.729619192542134	3.9433671856027983
9	79.55278665383157	5.7963028639613965	10.34988443174135	4.30102605046568
10	45.61174306284712	26.08292612192058	14.839837942378347	13.465492872853952
11	40.07197902126002	22.39581822851243	22.521711633172544	15.010491117055011
12	36.54675404314199	20.529150630078497	25.968703102260875	16.95539222451864
13	23.443706989477437	33.895864525698435	26.844750685285707	15.815677799538424
14	17.563719777983156	36.46289498924683	27.522786077300577	18.450599155469444
15	15.424719902024671	23.170710827776215	44.2604592331439	17.144110037055217
16	17.141279793986257	19.884134740534844	42.00985693295993	20.96472853251897
17	17.361444751733522	21.545347656925625	29.320863957902755	31.772343633438098
18	21.599436746688006	23.239055956700774	32.61156311529397	22.54994418131725
19	27.130849824158048	27.431030295830283	24.28393976823454	21.15418011177713
20	29.003597203999377	24.318985864508228	24.25539274863773	22.422024182854667
21	23.283815726717318	29.95207804482616	25.763877363121935	21.000228865334588
22	22.356823768451175	25.832641787315968	24.226775846496	27.583758597736853
23	19.99147432952066	30.534653880490364	24.772174168266687	24.70169762172229
24	20.229983455307494	24.3686024713962	35.05151217091873	20.349901902377578
25	19.291565700948134	25.797840279949476	31.010728717707714	23.899865301394684
26	18.01882985172671	31.071910885778724	27.385676524181978	23.52358273831258
27	20.385437176465658	29.692394507930796	26.98909308180276	22.93307523380079
28	17.943321761948607	28.19281991792295	33.8232565615342	20.040601758594242
29	18.909762416817934	24.69942643901263	31.67069947186267	24.72011167230677
30	21.034331547251956	27.922409410383498	29.049894390004	21.993364652360544
31	26.540342319646253	26.09756651507978	23.904198019179265	23.4578931460947
32	27.20254931523841	25.886940524713086	23.56725932888298	23.34325083116552
33	24.531184212135454	28.14131648232234	23.30865897143376	24.018840334108447
34	18.961405617508373	26.095609803822228	27.691133127995123	27.25185145067428
35	19.281048377938777	25.207647246889792	29.094164982206156	26.41713939296527
36	25.28755793699739	25.421557716867376	26.247359750100017	23.043524596035216
37	20.04441035729198	29.09954593816443	28.39222975983116	22.463813944712424
38	19.910375636149542	28.84835313047595	28.00941317879973	23.231858054574772
39	20.048044249627438	27.625862831046717	27.240775067305627	25.085317852020218
40	23.311803685954832	23.351462030192753	28.572212254253664	24.76452202959875
41	18.192208445654963	22.731953269542217	28.516480924685833	30.559357360116984
42	21.962616332598984	25.64053467135113	24.925671178167295	27.47117781788259
43	21.604957467736103	25.49336203176511	26.612286399633817	26.28939410086497
44	21.00676288328392	28.066297570358618	27.27854658283091	23.64839296352655
45	19.103406948770854	29.89652142162061	26.111193611337747	24.88887801827079
46	22.544283695179328	29.071173624929898	26.59572423648952	21.788818443401254
47	21.350200650257083	25.06830145233399	28.36197061788399	25.21952727952494
48	22.28348203756536	22.560426563054147	29.681388007107056	25.474703392273433
49	21.415366123388115	21.91481667187874	30.175387717094495	26.494429487638648
50	19.38272748078667	27.05911539180522	28.98595186140894	24.572205265999173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3502.0
1	3711.0
2	3920.0
3	14028.0
4	24136.0
5	16767.5
6	9399.0
7	9883.5
8	10368.0
9	11111.5
10	11855.0
11	12343.5
12	12832.0
13	12480.0
14	12128.0
15	11713.5
16	11299.0
17	10656.5
18	10014.0
19	9669.0
20	9324.0
21	9416.5
22	9509.0
23	10550.0
24	11591.0
25	11979.5
26	12368.0
27	14622.0
28	16876.0
29	21927.0
30	26978.0
31	31626.0
32	36274.0
33	42841.0
34	49408.0
35	58171.5
36	66935.0
37	70905.0
38	74875.0
39	93724.0
40	112573.0
41	150410.0
42	188247.0
43	215233.5
44	242220.0
45	262695.0
46	283170.0
47	287977.5
48	292785.0
49	286160.5
50	279536.0
51	249867.0
52	220198.0
53	191373.5
54	162549.0
55	146673.5
56	130798.0
57	127271.5
58	123745.0
59	117192.0
60	110639.0
61	101767.5
62	92896.0
63	79719.0
64	66542.0
65	54853.5
66	43165.0
67	36408.0
68	29651.0
69	26630.0
70	23609.0
71	19538.0
72	15467.0
73	12206.0
74	8945.0
75	7169.5
76	5394.0
77	4220.5
78	3047.0
79	2366.0
80	1685.0
81	1299.5
82	914.0
83	642.5
84	371.0
85	259.0
86	147.0
87	87.5
88	28.0
89	21.5
90	15.0
91	14.5
92	14.0
93	8.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2861945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.608699008645036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17684767940514	17.109086699576007
2	8.369745075884975	3.6171860424777464
3	2.8718899176310404	1.8617341444815458
4	1.4581951398153574	1.260387994885565
5	0.8814321703576643	0.9523301232897751
6	0.5833086877133008	0.7562725117154664
7	0.44178999248497486	0.6682554880847561
8	0.35218744095912885	0.6088249925048608
9	0.29146467406286997	0.5668355172129648
>10	3.2434811257464986	16.375227218280347
>50	1.2728711070864853	19.99901053124333
>100	1.047504147919805	33.721096989056
>500	0.006351417480331397	0.903957596667632
>1k	0.002768566593990609	1.0992690250752062
>5k	0.0	0.0
>10k+	1.6285685847003582E-4	0.5005251254488158
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14223	0.4969697181462257	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4985	0.17418224319475042	No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2901	0.10136463139578154	TruSeq Adapter, Index 27 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09647285325189688	0.0
2	0.0	0.0	0.0	0.38529741137583007	0.0
3	3.4941272456319045E-5	0.0	0.0	0.6058467231201159	0.0
4	3.4941272456319045E-5	0.0	0.0	0.9243713628319202	0.0
5	3.4941272456319045E-5	0.0	0.0	1.5445090663866705	0.0
6	3.4941272456319045E-5	0.0	0.0	2.586632517396386	0.0
7	3.4941272456319045E-5	0.0	0.0	3.2567012992912163	0.0
8	3.4941272456319045E-5	0.0	0.0	5.326237925606537	0.0
9	3.4941272456319045E-5	0.0	0.0	6.420808226573187	0.0
10	3.4941272456319045E-5	0.0	0.0	7.936525684455851	0.0
11	3.4941272456319045E-5	0.0	0.0	8.728120211953758	0.0
12	6.988254491263809E-5	0.0	0.0	9.540225266383526	0.0
13	6.988254491263809E-5	0.0	0.0	9.92538291266953	0.0
14	1.0482381736895713E-4	0.0	0.0	10.084190995983501	0.0
15	1.0482381736895713E-4	0.0	0.0	10.247087208174861	0.0
16	1.0482381736895713E-4	0.0	0.0	10.583396955566931	0.0
17	1.0482381736895713E-4	0.0	0.0	10.924668363647799	0.0
18	1.0482381736895713E-4	0.0	0.0	11.531633207486516	0.0
19	1.0482381736895713E-4	0.0	0.0	11.73160210975403	0.0
20	1.3976508982527618E-4	0.0	0.0	12.00044026003295	0.0
21	1.3976508982527618E-4	0.0	0.0	12.246147287945785	0.0
22	1.3976508982527618E-4	0.0	0.0	12.488709601337552	0.0
23	1.3976508982527618E-4	0.0	0.0	12.780154754895708	0.0
24	1.3976508982527618E-4	0.0	0.0	12.994903815412245	0.0
25	1.7470636228159522E-4	0.0	0.0	13.171007828592094	0.0
26	1.7470636228159522E-4	0.0	0.0	13.352003619915827	0.0
27	1.7470636228159522E-4	0.0	0.0	13.542503437347678	0.0
28	2.0964763473791425E-4	0.0	0.0	13.721472634868944	0.0
29	2.0964763473791425E-4	0.0	0.0	13.9111688030343	0.0
30	2.0964763473791425E-4	0.0	0.0	14.19195686849328	0.0
31	2.0964763473791425E-4	0.0	0.0	14.40876746408474	0.0
32	2.0964763473791425E-4	0.0	0.0	14.612684730139817	0.0
33	2.0964763473791425E-4	0.0	0.0	14.803988196838164	0.0
34	2.0964763473791425E-4	0.0	0.0	14.987185288326645	0.0
35	2.0964763473791425E-4	0.0	0.0	15.232962198784394	0.0
36	2.0964763473791425E-4	0.0	0.0	15.430590035797334	0.0
37	2.0964763473791425E-4	0.0	0.0	15.63527600984645	0.0
38	2.0964763473791425E-4	0.0	0.0	15.844713996949627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	20	7.858449E-4	44.000004	6
CTATCGT	75	0.0	44.0	13
CGTTACA	30	2.5290447E-6	44.0	38
CGACTCA	25	4.444508E-5	44.0	18
TACGTAA	25	4.444508E-5	44.0	29
GTACGAC	25	4.444508E-5	44.0	36
CGGTCTA	525	0.0	41.485714	31
CGTTTTT	9060	0.0	41.42605	1
TTACGTC	60	3.6379788E-12	40.333332	15
ACGGTCT	540	0.0	39.925926	30
CGACGGT	560	0.0	39.285713	28
TCTAGCG	45	2.3530447E-8	39.11111	1
CGCATCG	215	0.0	38.883724	21
GCGTAAG	275	0.0	38.4	1
ACGATTG	75	0.0	38.13333	1
ATATCGA	75	0.0	38.13333	14
TACGGGA	650	0.0	37.907692	4
ACGGGTA	325	0.0	37.907692	5
GTATCGC	35	7.294011E-6	37.714287	31
TATAGCG	170	0.0	37.52941	1
>>END_MODULE