FastQCFastQC Report
Thu 26 May 2016
SRR1547231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547231_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1802763
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156140.8661149579839391No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20580.11415810064883737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT207.8576733E-444.00000423
CTTAACG408.3164196E-944.0000041
CCGGATA351.4466968E-743.99999620
CGTTTTT115500.043.0666661
CGACGGT1750.041.48571428
GCGAACG800.041.2500041
TACGCAC650.040.61538713
ATCGTAG603.6379788E-1240.3333321
TAACGCG501.3496901E-939.6000021
GCGACGT950.039.3684217
CGACGTC950.039.3684218
AGGGCGA18550.039.1374666
GCGTAAG1300.038.923081
GACCGAT8600.038.883729
GGACCGA8850.038.5310758
TCGGTAC404.1262865E-738.50000436
GGGACCG11150.038.4753387
CGAACGG1550.038.322582
AACGCGG1150.038.260872
AGCGAAG2100.037.7142871
Produced by FastQC (version 0.11.5)