##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547231_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1802763
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.447368844379433	31.0	31.0	33.0	30.0	34.0
2	31.82898251184432	31.0	31.0	34.0	30.0	34.0
3	31.92805709901967	33.0	31.0	34.0	30.0	34.0
4	35.608658487000234	37.0	35.0	37.0	33.0	37.0
5	35.50051670685498	37.0	35.0	37.0	33.0	37.0
6	35.54206848043808	37.0	35.0	37.0	33.0	37.0
7	36.05930452311258	37.0	35.0	37.0	35.0	37.0
8	36.06612017220234	37.0	35.0	37.0	35.0	37.0
9	37.7877741000897	39.0	38.0	39.0	35.0	39.0
10	37.12704720476291	39.0	37.0	39.0	33.0	39.0
11	36.86053574429917	39.0	37.0	39.0	32.0	39.0
12	36.558689078930506	39.0	35.0	39.0	32.0	39.0
13	36.45358430364946	39.0	35.0	39.0	32.0	39.0
14	37.55215688362807	40.0	36.0	41.0	32.0	41.0
15	37.71629881465284	40.0	36.0	41.0	33.0	41.0
16	37.74397854848363	40.0	36.0	41.0	33.0	41.0
17	37.68445269844123	40.0	36.0	41.0	33.0	41.0
18	37.57874495982001	39.0	36.0	41.0	33.0	41.0
19	37.52511783301521	39.0	36.0	41.0	33.0	41.0
20	37.337130837497774	39.0	35.0	41.0	32.0	41.0
21	37.25744981453469	39.0	35.0	41.0	32.0	41.0
22	37.19900785627395	39.0	35.0	41.0	32.0	41.0
23	37.09050052613682	39.0	35.0	41.0	32.0	41.0
24	37.04131325082665	39.0	35.0	41.0	32.0	41.0
25	36.90250631946628	38.0	35.0	41.0	32.0	41.0
26	36.809051994077976	38.0	35.0	41.0	31.0	41.0
27	36.67234295356628	38.0	35.0	41.0	31.0	41.0
28	36.74116786288602	38.0	35.0	41.0	31.0	41.0
29	36.68147615632227	38.0	35.0	41.0	31.0	41.0
30	36.64330308531959	38.0	35.0	41.0	31.0	41.0
31	36.47532981318121	38.0	35.0	40.0	31.0	41.0
32	36.214012047063314	38.0	35.0	41.0	30.0	41.0
33	36.053491224304025	38.0	35.0	41.0	30.0	41.0
34	35.91370080260134	39.0	35.0	41.0	29.0	41.0
35	35.6922812371898	38.0	35.0	41.0	27.0	41.0
36	35.54355786090573	38.0	35.0	41.0	26.0	41.0
37	35.50111356845021	38.0	35.0	41.0	26.0	41.0
38	35.4601486717888	38.0	35.0	41.0	26.0	41.0
39	35.373203798835455	38.0	35.0	41.0	25.0	41.0
40	35.27719506113671	38.0	35.0	40.0	25.0	41.0
41	35.28671600204797	38.0	35.0	40.0	25.0	41.0
42	35.10528949174129	38.0	34.0	40.0	24.0	41.0
43	35.132407865038275	38.0	34.0	40.0	24.0	41.0
44	34.799226520624174	38.0	34.0	40.0	23.0	41.0
45	34.73141061803465	38.0	34.0	40.0	23.0	41.0
46	34.76029683324985	38.0	34.0	40.0	23.0	41.0
47	34.68814148060505	38.0	34.0	40.0	23.0	41.0
48	34.655862695207304	38.0	34.0	40.0	23.0	41.0
49	34.652559432382404	38.0	34.0	40.0	24.0	41.0
50	34.093714481604074	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	18.0
15	27.0
16	97.0
17	237.0
18	610.0
19	1277.0
20	2314.0
21	3805.0
22	6071.0
23	8890.0
24	13071.0
25	19909.0
26	28955.0
27	35118.0
28	36546.0
29	37373.0
30	41752.0
31	50055.0
32	61596.0
33	77681.0
34	120566.0
35	185382.0
36	129648.0
37	163999.0
38	256198.0
39	521560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.861148692312856	23.594892950432197	28.590779819643515	15.953178537611434
2	33.57102403366388	25.138911770432387	28.261618415731853	13.028445780171879
3	27.845590352142796	25.17097366653298	34.02366256684878	12.959773414475448
4	23.973644899523674	27.848918576651506	33.8023356370194	14.37510088680542
5	21.791716382020265	32.090407890554665	32.524297425673815	13.593578301751256
6	18.881627812419048	40.37219534680931	29.961009849880433	10.785166990891204
7	85.90491373519426	3.2106272427379525	9.217573247287635	1.6668857747801569
8	86.23596113299419	2.574936361573873	8.808368043941439	2.380734461490501
9	80.48240395437448	5.498171417984505	11.09713256817452	2.9222920594664967
10	37.05162575446689	36.87034845955902	15.733016486360105	10.34500929961398
11	28.15539258349545	24.624423731793918	32.95657831894708	14.263605365763555
12	27.611727109997265	23.38682344822919	32.223093107635336	16.77835633413821
13	21.129843468054315	30.36117337664463	32.839757638691275	15.66922551660978
14	17.132479421865217	34.030929190359466	32.13533892142229	16.701252466353036
15	16.184545611375427	26.063936302220537	41.683626744058984	16.067891342345057
16	18.478746235639402	24.54793003850201	39.73733652177241	17.23598720408617
17	19.03250732348068	23.91612208593143	32.50987511946939	24.5414954711185
18	20.962933008942382	25.184064682933922	34.710663575855506	19.14233873226819
19	23.810950191456115	26.9535152429909	30.64174270272909	18.5937918628239
20	25.02209109017658	26.013513700913542	29.68704150240492	19.27735370650496
21	22.233538185551843	28.318031821154527	31.33523374952781	18.11319624376582
22	21.98042671166426	25.82241814370497	30.44437898936244	21.75277615526833
23	18.833091205000326	29.965835775418064	30.408323223851387	20.79274979573022
24	18.744504962660095	26.459995018757322	35.80803466678648	18.987465351796104
25	18.715993172702124	28.00218331527772	33.7002146150104	19.581608897009755
26	18.23672884344753	30.872610542816776	31.098818868592264	19.791841745143426
27	19.09757411262601	29.19196810673394	31.779773603074833	19.930684177565215
28	17.612631277655467	28.70604732846192	35.86067608443262	17.820645309449993
29	18.621638007880126	26.30595369441241	34.635501172367086	20.43690712534038
30	19.991202393215303	28.648524514869678	32.38673081264703	18.973542279267992
31	23.358256187862743	27.74585455769838	28.783816841148834	20.112072413290043
32	24.36310263745151	28.675483133390244	28.790140467715393	18.17127376144285
33	22.262105445918294	29.64127841541012	28.618847846333654	19.477768292337927
34	20.519003329888623	28.736334171491208	30.65267037319936	20.091992125420813
35	20.429973324280564	29.122130862459457	30.17063252352084	20.27726328973914
36	24.160302824053968	28.176471338717292	29.138161810509754	18.525064026718987
37	22.109617293010782	30.059913588197674	29.19047040570502	18.639998713086523
38	22.19082597102337	29.76164920180856	28.622120600433888	19.425404226734187
39	21.58164994511203	29.350336122940174	27.97589034165889	21.092123590288907
40	22.685954837102827	26.844848712781438	28.87994705904215	21.589249391073594
41	19.44193440846079	26.584637026608597	28.722632980597005	25.250795584333602
42	21.65819910881242	27.967126017119277	27.83510644494035	22.539568429127954
43	21.442419219830892	27.981770204957613	28.61069369628731	21.965116878924185
44	21.380680655194276	29.962174728458486	28.776993980905974	19.880150635441264
45	19.389182049997697	31.051391669343115	28.16021850903308	21.39920777162611
46	22.02929614153386	29.49638970846418	28.593331458433525	19.88098269156844
47	21.7342490388365	27.095408547879007	29.308123142088004	21.862219271196494
48	22.20130987822581	25.67331368571465	30.10939319256053	22.015983243499008
49	21.594519079879053	25.29195462742468	30.35934285316484	22.75418343953143
50	19.822516881032058	28.66960327009152	29.865434336071907	21.64244551280451
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1447.0
1	1587.0
2	1727.0
3	14199.0
4	26671.0
5	19205.5
6	11740.0
7	11837.0
8	11934.0
9	12319.0
10	12704.0
11	12748.0
12	12792.0
13	12566.0
14	12340.0
15	11761.5
16	11183.0
17	10543.0
18	9903.0
19	9820.0
20	9737.0
21	10130.0
22	10523.0
23	11365.5
24	12208.0
25	14117.0
26	16026.0
27	19110.0
28	22194.0
29	25842.0
30	29490.0
31	34679.5
32	39869.0
33	46286.0
34	52703.0
35	59747.5
36	66792.0
37	73381.0
38	79970.0
39	91827.0
40	103684.0
41	117543.5
42	131403.0
43	145191.0
44	158979.0
45	163914.0
46	168849.0
47	170762.5
48	172676.0
49	166094.0
50	159512.0
51	142725.5
52	125939.0
53	110367.0
54	94795.0
55	81644.0
56	68493.0
57	59435.0
58	50377.0
59	45551.0
60	40725.0
61	35190.5
62	29656.0
63	24619.5
64	19583.0
65	15263.0
66	10943.0
67	8294.0
68	5645.0
69	4590.0
70	3535.0
71	3059.5
72	2584.0
73	1978.5
74	1373.0
75	1169.5
76	966.0
77	800.5
78	635.0
79	465.5
80	296.0
81	192.0
82	88.0
83	71.5
84	55.0
85	32.0
86	9.0
87	7.5
88	6.0
89	3.0
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1802763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.32268523099895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84828759073433	20.99701572382917
2	7.027279644390442	3.8400829950777258
3	2.8301655081843853	2.319831639952564
4	1.6325175097848272	1.7841904821578036
5	1.1711577406466174	1.599958715176772
6	0.9130050278956315	1.4967449394907055
7	0.7228816536632018	1.3825747517612563
8	0.5837010400378522	1.2758623828768763
9	0.5201256350467302	1.2790106106259722
>10	6.708179100570466	42.18935768677651
>50	0.9601185916344762	17.321981141078368
>100	0.08053687828753292	3.1627190492408217
>500	0.0014308553865133838	0.2953333747841665
>1k	4.0881582471810965E-4	0.1832988154390383
>5k	0.0	0.0
>10k+	2.0440791235905482E-4	0.8720376917322195
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15614	0.8661149579839391	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2058	0.11415810064883737	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05890957380421054	0.0
2	0.0	0.0	0.0	0.21228525324737638	0.0
3	0.0	0.0	0.0	0.39411725224003374	0.0
4	0.0	0.0	0.0	0.5793329461498822	0.0
5	0.0	0.0	0.0	0.9345099716379801	0.0
6	0.0	0.0	0.0	1.6735422237975819	0.0
7	0.0	0.0	0.0	2.151475263248691	0.0
8	0.0	0.0	0.0	3.4871472290034795	0.0
9	0.0	0.0	0.0	4.301230943834548	0.0
10	0.0	0.0	0.0	5.368592543778633	0.0
11	0.0	0.0	0.0	5.905878920301781	0.0
12	0.0	0.0	0.0	6.46707304287918	0.0
13	0.0	0.0	0.0	6.792074166154952	0.0
14	0.0	0.0	0.0	6.951107827262929	0.0
15	0.0	0.0	0.0	7.084070396385992	0.0
16	0.0	0.0	0.0	7.317434404855214	0.0
17	0.0	0.0	0.0	7.577757031845007	0.0
18	0.0	0.0	0.0	8.034001141581006	0.0
19	0.0	0.0	0.0	8.205127351737305	0.0
20	0.0	0.0	0.0	8.419575950915345	0.0
21	0.0	0.0	0.0	8.610116804039134	0.0
22	0.0	0.0	0.0	8.821570001159332	0.0
23	5.547040847854099E-5	0.0	0.0	9.047279093258515	0.0
24	5.547040847854099E-5	0.0	0.0	9.223286699360925	0.0
25	5.547040847854099E-5	0.0	0.0	9.384206354357174	0.0
26	5.547040847854099E-5	0.0	0.0	9.542907193014278	0.0
27	1.1094081695708199E-4	0.0	0.0	9.740714669648757	0.0
28	1.1094081695708199E-4	0.0	0.0	9.912561995115276	0.0
29	1.1094081695708199E-4	0.0	0.0	10.090455595105956	0.0
30	1.1094081695708199E-4	0.0	0.0	10.355437736407946	0.0
31	1.1094081695708199E-4	0.0	0.0	10.558681313073322	0.0
32	1.1094081695708199E-4	0.0	0.0	10.760926422386081	0.0
33	1.1094081695708199E-4	0.0	0.0	10.95718072758316	0.0
34	1.6641122543562299E-4	0.0	0.0	11.15071698276479	0.0
35	1.6641122543562299E-4	0.0	0.0	11.36050606763063	0.0
36	1.6641122543562299E-4	0.0	0.0	11.561863650407735	0.0
37	1.6641122543562299E-4	0.0	0.0	11.766050224017244	0.0
38	1.6641122543562299E-4	0.0	0.0	11.967851570062177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.8576733E-4	44.000004	23
CTTAACG	40	8.3164196E-9	44.000004	1
CCGGATA	35	1.4466968E-7	43.999996	20
CGTTTTT	11550	0.0	43.066666	1
CGACGGT	175	0.0	41.485714	28
GCGAACG	80	0.0	41.250004	1
TACGCAC	65	0.0	40.615387	13
ATCGTAG	60	3.6379788E-12	40.333332	1
TAACGCG	50	1.3496901E-9	39.600002	1
GCGACGT	95	0.0	39.36842	17
CGACGTC	95	0.0	39.36842	18
AGGGCGA	1855	0.0	39.137466	6
GCGTAAG	130	0.0	38.92308	1
GACCGAT	860	0.0	38.88372	9
GGACCGA	885	0.0	38.531075	8
TCGGTAC	40	4.1262865E-7	38.500004	36
GGGACCG	1115	0.0	38.475338	7
CGAACGG	155	0.0	38.32258	2
AACGCGG	115	0.0	38.26087	2
AGCGAAG	210	0.0	37.714287	1
>>END_MODULE