FastQCFastQC Report
Thu 26 May 2016
SRR1547230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547230_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1839818
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246441.3394803181619053No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC30330.16485326265967612No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC25630.139307257565694No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATG254.443887E-544.01
CGTTTTT150250.043.0043261
CGGTCTA2350.040.25531831
GCGTAAG1800.039.1111071
GTACGAG2600.038.923081
TATAGCG750.038.1333351
GTTTTTT174850.037.9353752
ACCCGCT9700.037.19587734
CGGGATA4650.036.9032256
CGTAAGG2700.036.6666682
ACGTAGG2350.036.5106352
AATACGG1450.036.4137952
AAGGGAT18550.036.053915
GCGCGAC10700.035.981319
AACGGGA5750.035.9652184
TATACTA10000.035.8644
CGACCCG10150.035.7635532
CATATGC9550.035.70680633
AGGGCGC7150.035.6923076
TAGGGAT17450.035.679085
Produced by FastQC (version 0.11.5)