##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547229_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1681116
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33254754579696	31.0	31.0	33.0	30.0	34.0
2	31.713311871399714	31.0	31.0	34.0	30.0	34.0
3	31.839432852938167	31.0	31.0	34.0	30.0	34.0
4	35.52884393462438	37.0	35.0	37.0	33.0	37.0
5	35.39621061247409	37.0	35.0	37.0	33.0	37.0
6	35.43640474541911	37.0	35.0	37.0	33.0	37.0
7	35.95806357205571	37.0	35.0	37.0	35.0	37.0
8	35.95899509611473	37.0	35.0	37.0	35.0	37.0
9	37.65820978445271	39.0	37.0	39.0	35.0	39.0
10	36.98829824949617	39.0	37.0	39.0	32.0	39.0
11	36.73896328391378	39.0	35.0	39.0	32.0	39.0
12	36.10477801650808	38.0	35.0	39.0	32.0	39.0
13	35.82773824054973	38.0	35.0	39.0	31.0	39.0
14	36.82656818446794	39.0	35.0	41.0	31.0	41.0
15	37.08249817383214	39.0	35.0	41.0	32.0	41.0
16	37.174438884645674	39.0	35.0	41.0	32.0	41.0
17	37.12010473994656	39.0	35.0	41.0	32.0	41.0
18	37.08478415528732	38.0	35.0	41.0	32.0	41.0
19	37.043917254966345	38.0	35.0	41.0	32.0	41.0
20	36.880791093535485	38.0	35.0	41.0	32.0	41.0
21	36.756012077691246	38.0	35.0	41.0	31.0	41.0
22	36.688753185383995	38.0	35.0	41.0	31.0	41.0
23	36.59731392717695	38.0	35.0	40.0	31.0	41.0
24	36.54525684128876	38.0	35.0	40.0	31.0	41.0
25	36.37757061380654	38.0	35.0	40.0	31.0	41.0
26	36.254446451048	38.0	35.0	40.0	31.0	41.0
27	36.14285153433791	38.0	35.0	40.0	30.0	41.0
28	36.235912929268416	38.0	35.0	40.0	31.0	41.0
29	36.18976263386941	38.0	35.0	40.0	30.0	41.0
30	36.18312180718047	38.0	35.0	40.0	30.0	41.0
31	36.0529933686908	38.0	35.0	40.0	30.0	41.0
32	35.81315328626936	38.0	35.0	40.0	30.0	41.0
33	35.711450012967575	38.0	35.0	40.0	29.0	41.0
34	35.62910114471577	38.0	35.0	40.0	29.0	41.0
35	35.46377882311512	38.0	35.0	40.0	28.0	41.0
36	35.25582708153394	37.0	34.0	40.0	27.0	41.0
37	35.19852050661584	37.0	34.0	40.0	27.0	41.0
38	35.16011506642016	37.0	34.0	40.0	27.0	41.0
39	35.021696301742416	37.0	34.0	40.0	26.0	41.0
40	34.901449989173855	37.0	34.0	40.0	26.0	41.0
41	34.90704805617221	37.0	34.0	40.0	26.0	41.0
42	34.73639534690051	37.0	34.0	40.0	25.0	41.0
43	34.766284420587276	37.0	34.0	40.0	26.0	41.0
44	34.52845312280652	36.0	34.0	40.0	25.0	41.0
45	34.44353988659914	36.0	34.0	40.0	24.0	41.0
46	34.44513703991872	36.0	34.0	40.0	24.0	41.0
47	34.343495035440746	36.0	33.0	40.0	24.0	41.0
48	34.32444935388159	36.0	34.0	40.0	24.0	41.0
49	34.30867411885914	36.0	34.0	40.0	24.0	41.0
50	33.753149098574994	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	2.0
12	5.0
13	7.0
14	18.0
15	37.0
16	121.0
17	305.0
18	719.0
19	1301.0
20	2436.0
21	3954.0
22	6093.0
23	8903.0
24	12746.0
25	17977.0
26	24923.0
27	30155.0
28	33514.0
29	37111.0
30	43703.0
31	53132.0
32	65393.0
33	84993.0
34	140845.0
35	229490.0
36	124466.0
37	153244.0
38	223691.0
39	381831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.11734585834648	23.711034812588778	28.934588689894092	14.237030639170648
2	31.78323209106332	25.531968049795495	29.404455135755057	13.280344723386131
3	28.391497076941747	25.782694353036913	32.27701122349677	13.548797346524571
4	25.50353455680631	28.505647438963166	31.171079211666537	14.819738792563989
5	22.50493124805189	32.055908099143664	30.480288094337332	14.958872558467112
6	19.52637414669779	41.15480430856645	27.934836144561114	11.383985400174645
7	85.94861984538842	4.212499315930608	7.637426566637877	2.201454272043095
8	86.00072808777026	3.4632946209541755	7.113429412366547	3.4225478789090102
9	80.61281910350029	5.35816683679175	10.121669176903914	3.9073448828040416
10	44.28671192231827	28.11096914192715	14.827293298023456	12.775025637731128
11	39.611186854446686	22.54460727278784	23.596646513387533	14.247559359377938
12	36.43466601947753	20.51262375707566	26.222045355585216	16.830664867861586
13	23.114109912700847	33.70356358514225	27.114785654291556	16.067540847865345
14	17.305171088729153	36.83767211780746	27.81699775625239	18.040159037210994
15	15.93893580216951	23.64691074262573	43.532153640795755	16.881999814409
16	17.177755728932446	20.420423099893167	41.62009046371577	20.781730707458618
17	17.65065587383619	21.201987251325903	29.961466073727216	31.185890801110688
18	22.07468134263192	23.012867642684977	33.09920314838477	21.81324786629834
19	26.568600858001474	27.205975078459787	25.10314576745448	21.122278296084268
20	29.477501849961573	23.956823919348814	25.09707836936892	21.468595861320694
21	23.07056740879273	29.352644314847993	26.177610587252754	21.399177689106523
22	22.741797710568456	25.496039535641803	24.979180496765245	26.7829822570245
23	20.197356993806494	30.74927607613038	24.748678853809018	24.30468807625411
24	20.83794336619246	24.693536912384392	34.34337666169378	20.12514305972937
25	19.638085652625996	26.25000297421475	31.28862017850047	22.823291194658786
26	18.96573466673329	30.87764318464639	27.305670756806787	22.850951391813535
27	20.296160407729154	29.44532084639014	27.310905374762957	22.947613371117757
28	18.23752792787648	27.78190202222809	34.226014147744706	19.754555902150713
29	19.255363698876224	24.321105741662087	32.929375486284115	23.49415507317758
30	21.814140130722688	27.9333490371872	29.40623966460375	20.84627116748636
31	27.911339848053316	25.545708921930434	23.911794308066785	22.631156921949465
32	30.166746375621905	25.77585365911692	24.206003630921362	19.851396334339807
33	27.297878314167495	25.876857991952967	23.989778218754683	22.83548547512486
34	21.415535870219546	25.856633331667773	28.57464922111264	24.15318157700004
35	22.932444875903865	24.619716902343445	28.654774566418972	23.793063655333718
36	28.740967309810866	26.183915922518135	25.332636177396445	19.742480590274557
37	24.155739401683167	29.863019565574294	26.569909512490515	19.411331520252023
38	23.772006214919138	30.621801232038713	25.081969358449985	20.524223194592164
39	23.563692213981664	28.83947330225874	26.109917459592317	21.48691702416728
40	25.715417615441172	25.366244804046833	26.430478325112606	22.48785925539939
41	20.24898936182869	23.685218628577683	27.74341568339127	28.32237632620236
42	23.41343488492168	25.984167660054393	24.584145293959487	26.01825216106444
43	22.314581504191263	25.84782965601422	26.67174662545595	25.165842214338568
44	22.759702483350345	28.13571460862903	27.251718501281292	21.852864406739332
45	19.99421812653023	30.63768353879209	25.065016334387398	24.30308200029028
46	22.930422409875344	29.595756628334986	26.162917966398513	21.310902995391157
47	23.404869146447954	25.77347428731866	27.109432067745477	23.71222449848791
48	23.227308526002965	23.434789746811045	28.727702312035575	24.61019941515041
49	21.9688587819044	23.156462730709837	29.94106296055715	24.933615526828607
50	20.875358987720062	27.698742977878982	28.189904801334354	23.23599323306661
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1655.0
1	1831.5
2	2008.0
3	9120.0
4	16232.0
5	11477.5
6	6723.0
7	6916.5
8	7110.0
9	7705.0
10	8300.0
11	8413.0
12	8526.0
13	8440.0
14	8354.0
15	7717.5
16	7081.0
17	6756.0
18	6431.0
19	6527.5
20	6624.0
21	6554.5
22	6485.0
23	6615.0
24	6745.0
25	7840.5
26	8936.0
27	9767.5
28	10599.0
29	13552.5
30	16506.0
31	19043.0
32	21580.0
33	25161.5
34	28743.0
35	35152.5
36	41562.0
37	44446.0
38	47330.0
39	56169.5
40	65009.0
41	84914.0
42	104819.0
43	119387.5
44	133956.0
45	139259.5
46	144563.0
47	144620.0
48	144677.0
49	156032.5
50	167388.0
51	160053.0
52	152718.0
53	131224.0
54	109730.0
55	96239.0
56	82748.0
57	76832.5
58	70917.0
59	68616.0
60	66315.0
61	60107.0
62	53899.0
63	47102.5
64	40306.0
65	33125.5
66	25945.0
67	21736.0
68	17527.0
69	15119.0
70	12711.0
71	10582.5
72	8454.0
73	6887.0
74	5320.0
75	4243.5
76	3167.0
77	2514.5
78	1862.0
79	1313.0
80	764.0
81	637.5
82	511.0
83	345.5
84	180.0
85	116.5
86	53.0
87	39.0
88	25.0
89	20.0
90	15.0
91	9.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1681116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.64819483770598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49044470161694	20.13138218605258
2	7.598457145035697	3.897734186436693
3	2.676683555291441	2.0595630403499525
4	1.5383304258095456	1.5782159394373767
5	1.0195832435151986	1.3075234841469017
6	0.7437169111459682	1.1444997744701193
7	0.5796980351622454	1.0407745707013618
8	0.4677604484068571	0.9597768894489428
9	0.38771537080161633	0.8949779434703948
>10	4.749598393250295	28.778310225270808
>50	1.4258576968647334	25.711234574626
>100	0.3186675572668617	11.02780342625996
>500	0.0018594751107341586	0.3463644567850155
>1k	0.001394606333050619	0.5230043682228814
>5k	0.0	0.0
>10k+	2.3243438884176982E-4	0.598834934321081
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10045	0.597519742837496	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2830	0.16834055472674103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06947765650912846	0.0
2	0.0	0.0	0.0	0.26416975390157493	0.0
3	0.0	0.0	0.0	0.4463106650582113	0.0
4	0.0	0.0	0.0	0.6589075352325479	0.0
5	0.0	0.0	0.0	1.0916557810406897	0.0
6	0.0	0.0	0.0	1.9522745604705445	0.0
7	0.0	0.0	0.0	2.495128236243067	0.0
8	0.0	0.0	0.0	4.191025485451331	0.0
9	0.0	0.0	0.0	5.2339041446277355	0.0
10	0.0	0.0	0.0	6.62553922513378	0.0
11	0.0	0.0	0.0	7.311274177391685	0.0
12	0.0	0.0	0.0	8.010631033194615	0.0
13	0.0	0.0	0.0	8.38193200231275	0.0
14	0.0	0.0	0.0	8.548547512485754	0.0
15	0.0	0.0	0.0	8.704039459501903	0.0
16	0.0	0.0	0.0	8.984329457336674	0.0
17	0.0	0.0	0.0	9.272114476335958	0.0
18	0.0	0.0	0.0	9.776600781861573	0.0
19	0.0	0.0	0.0	9.955410572500648	0.0
20	0.0	0.0	0.0	10.195786608419645	0.0
21	5.94842949564456E-5	0.0	0.0	10.419328588865968	0.0
22	5.94842949564456E-5	0.0	0.0	10.654945881188448	0.0
23	5.94842949564456E-5	0.0	0.0	10.93809112518113	0.0
24	1.189685899128912E-4	0.0	0.0	11.153721694398245	0.0
25	1.189685899128912E-4	0.0	0.0	11.329259848814717	0.0
26	1.784528848693368E-4	0.0	0.0	11.502061725663191	0.0
27	1.784528848693368E-4	0.0	0.0	11.705081624349539	0.0
28	1.784528848693368E-4	0.0	0.0	11.885378522362526	0.0
29	1.784528848693368E-4	0.0	0.0	12.083104318797751	0.0
30	1.784528848693368E-4	0.0	0.0	12.368212544523995	0.0
31	2.379371798257824E-4	0.0	0.0	12.573195424943906	0.0
32	2.379371798257824E-4	0.0	0.0	12.773360077472345	0.0
33	2.379371798257824E-4	0.0	0.0	12.960021795045671	0.0
34	2.379371798257824E-4	0.0	0.0	13.148705978647518	0.0
35	2.379371798257824E-4	0.0	0.0	13.38396636520026	0.0
36	2.379371798257824E-4	0.0	0.0	13.577528260988533	0.0
37	2.379371798257824E-4	0.0	0.0	13.773647981459934	0.0
38	2.379371798257824E-4	0.0	0.0	13.982735278231841	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATA	20	7.8575214E-4	44.000004	32
TAGCGCA	20	7.8575214E-4	44.000004	40
TAAATCG	20	7.8575214E-4	44.000004	34
AACGTAC	30	2.528419E-6	44.0	32
TAATCCG	25	4.4437224E-5	44.0	1
TCGATGG	110	0.0	42.0	2
TCACGAC	380	0.0	41.68421	25
CGGTCTA	375	0.0	41.65333	31
CGTTTTT	6825	0.0	41.485714	1
CTCACGA	390	0.0	40.615383	24
CGCATCG	60	3.6379788E-12	40.333332	21
CGTTGAT	455	0.0	40.131866	25
CGACGGT	395	0.0	39.544304	28
ACGGTCT	420	0.0	38.761906	30
TAGGGTA	885	0.0	38.53107	5
AGCGTGA	40	4.12605E-7	38.500004	40
GCGATCG	40	4.12605E-7	38.500004	9
TTACGAG	155	0.0	38.322582	1
TACGTAG	115	0.0	38.26087	1
AGGGCGC	530	0.0	38.18868	6
>>END_MODULE