FastQCFastQC Report
Thu 26 May 2016
SRR1547228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547228_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1453544
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220381.516156373663267No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC16900.11626755020831842No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG207.8571704E-444.026
TCGACTA207.8571704E-444.029
CGTTTTT154450.042.7892531
TAGTGCG603.6379788E-1240.3333321
TACGGGT950.039.368424
GTTTTTT171000.039.1239782
ACGGGTA1750.037.7142875
CGACGGT1350.037.4814828
CACGACG1350.037.4814826
GCGATAC1300.037.230779
GGTACGC301.301265E-436.6666649
AGGGCGA16350.036.599396
TACGGGA4300.036.325584
TAGGGCG7400.036.270275
CAACGAC1100.036.00000412
AGGGCGC4850.035.8350526
TTACGGG3350.035.462693
ACGGGAT3850.035.428575
TAGGGTA6600.035.3333325
GCGCGAC7350.035.3197259
Produced by FastQC (version 0.11.5)