##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547228_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1453544
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352286549289186	31.0	31.0	33.0	30.0	34.0
2	31.74983901416125	31.0	31.0	34.0	30.0	34.0
3	31.82066590347454	31.0	31.0	34.0	30.0	34.0
4	35.53693386646706	37.0	35.0	37.0	33.0	37.0
5	35.44512378022268	37.0	35.0	37.0	33.0	37.0
6	35.47135621625489	37.0	35.0	37.0	33.0	37.0
7	35.962867997116014	37.0	35.0	37.0	35.0	37.0
8	35.96497663641417	37.0	35.0	37.0	35.0	37.0
9	37.67151389982003	39.0	37.0	39.0	35.0	39.0
10	37.00778098220625	39.0	37.0	39.0	32.0	39.0
11	36.840486424903546	39.0	37.0	39.0	32.0	39.0
12	36.515377587468976	39.0	35.0	39.0	32.0	39.0
13	36.44246476198863	39.0	35.0	39.0	32.0	39.0
14	37.53944084252007	40.0	36.0	41.0	32.0	41.0
15	37.64350580374588	40.0	36.0	41.0	32.0	41.0
16	37.65204837280468	40.0	36.0	41.0	32.0	41.0
17	37.56807430666014	40.0	36.0	41.0	32.0	41.0
18	37.403085149125175	39.0	36.0	41.0	32.0	41.0
19	37.27938679530857	39.0	36.0	41.0	32.0	41.0
20	37.01662694765346	38.0	35.0	41.0	32.0	41.0
21	36.925493139526566	38.0	35.0	41.0	32.0	41.0
22	36.851674252723	38.0	35.0	41.0	31.0	41.0
23	36.74643698436373	38.0	35.0	41.0	31.0	41.0
24	36.67741740188119	38.0	35.0	41.0	31.0	41.0
25	36.51056658759556	38.0	35.0	40.0	31.0	41.0
26	36.41568676283621	38.0	35.0	40.0	31.0	41.0
27	36.32239271738592	38.0	35.0	40.0	31.0	41.0
28	36.34269000456815	38.0	35.0	40.0	31.0	41.0
29	36.20640723638225	38.0	35.0	40.0	30.0	41.0
30	36.1121644752412	38.0	35.0	40.0	30.0	41.0
31	35.899157507443874	38.0	35.0	40.0	30.0	41.0
32	35.534941494719114	38.0	34.0	40.0	27.0	41.0
33	35.20690808121392	38.0	34.0	40.0	25.0	41.0
34	34.8664643106779	38.0	34.0	40.0	23.0	41.0
35	34.54558995118139	38.0	34.0	40.0	21.0	41.0
36	34.36807623298641	38.0	34.0	40.0	21.0	41.0
37	34.328685612544234	38.0	34.0	40.0	20.0	41.0
38	34.26649004089315	38.0	33.0	40.0	20.0	41.0
39	34.17458639022967	38.0	33.0	40.0	19.0	41.0
40	34.08212616886726	37.0	33.0	40.0	18.0	41.0
41	34.044802221329384	37.0	33.0	40.0	18.0	41.0
42	33.840229810724686	37.0	33.0	40.0	18.0	41.0
43	33.852243894921656	37.0	33.0	40.0	18.0	41.0
44	33.50747001810747	37.0	33.0	40.0	18.0	41.0
45	33.44940572834396	36.0	33.0	40.0	18.0	41.0
46	33.45813817813565	36.0	33.0	40.0	18.0	41.0
47	33.3862621289758	36.0	33.0	40.0	18.0	41.0
48	33.335567413163965	36.0	33.0	40.0	18.0	41.0
49	33.297239712041744	36.0	33.0	40.0	18.0	41.0
50	32.75596129184944	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	6.0
14	16.0
15	44.0
16	96.0
17	316.0
18	682.0
19	1508.0
20	2559.0
21	4264.0
22	6322.0
23	9546.0
24	14140.0
25	22176.0
26	33313.0
27	39387.0
28	38429.0
29	37051.0
30	38568.0
31	44334.0
32	52894.0
33	66826.0
34	101893.0
35	140361.0
36	114473.0
37	141544.0
38	203804.0
39	338986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.621020072319794	22.84031305553874	26.205605059083183	20.33306181305829
2	38.854207371775466	23.151483546421712	25.76660906033804	12.227700021464779
3	27.365253477018925	23.921945259311038	35.63937520983197	13.073426053838066
4	24.18076095391677	25.822610117065597	35.75564276004029	14.24098616897734
5	21.30248551127451	29.486757882802312	34.79041570121028	14.420340904712894
6	18.310625615736434	38.80852592009599	32.72044052330029	10.160407940867287
7	80.53350982151211	3.3154139124787414	14.066584843664865	2.084491422344284
8	80.42625472637911	2.7087587303858705	13.678567693857222	3.1864188493777963
9	74.70238259041349	4.756443561392018	16.518798192555575	4.022375655638907
10	35.94469792452104	25.76158685254798	23.092730595014668	15.20098462791632
11	30.244079298597082	23.72979421331587	30.189798176044206	15.836328312042841
12	26.938021828028596	19.808894673983037	34.83623474762374	18.416848750364625
13	22.799378622181372	23.41291354097296	36.09302504774538	17.6946827891003
14	19.26780338262894	27.012666971209676	35.27069012014773	18.44883952601366
15	18.3676586329688	22.221549536856124	41.789997413219	17.620794416956073
16	20.51977786706147	20.737246344107916	39.61593181905742	19.12704396977319
17	20.37764250686598	21.601547665567743	34.59399921846191	23.42681060910437
18	22.66494856708844	22.564848398122106	35.604426147402485	19.165776887386965
19	25.03687538870512	25.82598118804797	30.786271347822975	18.35087207542393
20	25.622340981765944	23.6920932562069	30.774988579637082	19.910577182390078
21	23.861747563197262	26.48306484014244	31.35770227801842	18.297485318641886
22	21.99899005465263	23.325403290165276	32.1176379937587	22.557968661423388
23	20.598757244362744	26.186685783161707	31.52398551402641	21.690571458449142
24	21.010096701579037	23.614902610447295	34.82735988728239	20.547640800691276
25	22.00710814395711	23.77107263350817	32.898969690632	21.322849531902715
26	19.026737408705895	26.005611113251476	32.82184784223938	22.14580363580325
27	20.12900882257434	24.510300341785317	33.00065219903904	22.360038636601303
28	20.394360267043858	24.904165267786873	35.256655457282335	19.44481900788693
29	21.66456605372799	24.250521484041762	33.4470095160518	20.637902946178443
30	22.343320876423416	25.009631631378205	32.74912902533394	19.897918466864436
31	25.100306561067292	24.434554440732445	29.980998167238145	20.484140830962115
32	25.910601949442192	25.711158382546383	29.01556471630718	19.362674951704246
33	24.98734128447436	26.303434914938933	28.056391825772042	20.65283197481466
34	23.2979531407374	27.202341311993305	29.114839316869666	20.38486623039963
35	24.056512909137943	26.62547538980588	28.577738272800822	20.740273428255353
36	25.643186583963058	27.631636881993256	26.44020408050943	20.28497245353426
37	24.635167562867032	28.75124523234247	26.864890226921233	19.748696977869262
38	23.317766782429704	29.00338758235045	26.27756710495176	21.40127853026809
39	23.694638758785423	26.297724733479	27.407770249817	22.599866257918578
40	23.471322505545068	24.753705426185928	29.45174002300584	22.323232045263165
41	20.95265090014475	24.788241704413487	29.86294188548816	24.3961655099536
42	22.28759500916381	26.987418337525387	27.340211235435596	23.384775417875208
43	21.667730732609403	25.44498136967302	28.874392519249504	24.012895378468073
44	22.457386910888147	26.391220355214568	29.29894107092733	21.852451662969955
45	21.60278601817351	26.656778191785047	28.97731338026231	22.763122409779132
46	23.549476314442494	26.981295371863528	28.06719301238903	21.40203530130495
47	22.443077058554813	25.73186639000952	29.212944362193372	22.612112189242293
48	22.181715861370556	24.7184811742885	29.530994589775062	23.56880837456589
49	22.763328801880096	23.988334718453654	29.59593930421095	23.6523971754553
50	21.073596671308195	25.0606104803157	30.88451398788066	22.98127886049545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1225.0
1	1547.0
2	1869.0
3	19307.5
4	36746.0
5	25601.5
6	14457.0
7	14351.0
8	14245.0
9	14654.0
10	15063.0
11	14967.5
12	14872.0
13	14375.5
14	13879.0
15	13117.5
16	12356.0
17	11758.5
18	11161.0
19	10602.0
20	10043.0
21	9599.0
22	9155.0
23	9419.5
24	9684.0
25	10421.0
26	11158.0
27	11768.0
28	12378.0
29	13875.5
30	15373.0
31	17603.5
32	19834.0
33	22582.5
34	25331.0
35	28887.5
36	32444.0
37	36258.5
38	40073.0
39	45597.5
40	51122.0
41	60737.5
42	70353.0
43	79484.0
44	88615.0
45	97834.0
46	107053.0
47	116705.5
48	126358.0
49	126813.5
50	127269.0
51	114420.5
52	101572.0
53	90650.5
54	79729.0
55	75816.5
56	71904.0
57	69304.0
58	66704.0
59	64691.5
60	62679.0
61	58108.0
62	53537.0
63	47871.5
64	42206.0
65	35012.0
66	27818.0
67	22914.5
68	18011.0
69	16274.5
70	14538.0
71	12160.0
72	9782.0
73	8051.5
74	6321.0
75	4713.5
76	3106.0
77	2479.0
78	1852.0
79	1389.0
80	926.0
81	674.0
82	422.0
83	286.0
84	150.0
85	131.5
86	113.0
87	72.0
88	31.0
89	22.0
90	13.0
91	10.5
92	8.0
93	5.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1453544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.0611359794769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.90456540471352	27.372048545224725
2	7.626708126889026	5.500555176790535
3	3.3712351813064476	3.647117108756649
4	2.072625306385537	2.9896489203229533
5	1.440714042804617	2.5976892502559603
6	1.087110557560853	2.3521464984556126
7	0.9169071860350625	2.3145300301318943
8	0.7425088539875946	2.142057019968977
9	0.6590953426938662	2.1390954098691095
>10	6.020931643796596	41.73083957905065
>50	0.12818688121017172	2.938764730415953
>100	0.026335765854798375	1.8537118999217734
>500	0.002306780071398979	0.6025374788647656
>1k	5.766950178497448E-4	0.29156380995227843
>5k	0.0	0.0
>10k+	1.9223167261658158E-4	1.5276945420181436
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22038	1.516156373663267	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1690	0.11626755020831842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045475059578519814	0.0
2	0.0	0.0	0.0	0.18664725663619403	0.0
3	0.0	0.0	0.0	0.4089315493717424	0.0
4	0.0	0.0	0.0	0.6033529084774868	0.0
5	0.0	0.0	0.0	0.9963234687082057	0.0
6	0.0	0.0	0.0	1.9565283197481467	0.0
7	0.0	0.0	0.0	2.5749478515958235	0.0
8	0.0	0.0	0.0	4.154466600254275	0.0
9	0.0	0.0	0.0	5.206722328323051	0.0
10	0.0	0.0	0.0	6.535199484845316	0.0
11	0.0	0.0	0.0	7.147702443132096	0.0
12	0.0	0.0	0.0	7.795223261215347	0.0
13	0.0	0.0	0.0	8.231811352115932	0.0
14	0.0	0.0	0.0	8.451962926474877	0.0
15	0.0	0.0	0.0	8.602491565442808	0.0
16	0.0	0.0	0.0	8.829454079133484	0.0
17	0.0	0.0	0.0	9.044583445702367	0.0
18	0.0	0.0	0.0	9.461426692277634	0.0
19	0.0	0.0	0.0	9.62124297578883	0.0
20	0.0	0.0	0.0	9.833001271375341	0.0
21	0.0	0.0	0.0	10.01613986229519	0.0
22	0.0	0.0	0.0	10.20897888196023	0.0
23	0.0	0.0	0.0	10.430368808924944	0.0
24	0.0	0.0	0.0	10.617979228698958	0.0
25	0.0	0.0	0.0	10.784193667339963	0.0
26	0.0	0.0	0.0	10.93795578255629	0.0
27	0.0	0.0	0.0	11.138775296791842	0.0
28	0.0	0.0	0.0	11.306503277506563	0.0
29	0.0	0.0	0.0	11.484482065902373	0.0
30	0.0	0.0	0.0	11.73552365803856	0.0
31	0.0	0.0	0.0	11.926298756693983	0.0
32	0.0	0.0	0.0	12.132622060288508	0.0
33	0.0	0.0	0.0	12.348095413692327	0.0
34	0.0	0.0	0.0	12.56446313286698	0.0
35	0.0	0.0	0.0	12.780142878371759	0.0
36	0.0	0.0	0.0	12.982819921515963	0.0
37	0.0	0.0	0.0	13.202489914306	0.0
38	0.0	0.0	0.0	13.452499545937378	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCG	20	7.8571704E-4	44.0	26
TCGACTA	20	7.8571704E-4	44.0	29
CGTTTTT	15445	0.0	42.789253	1
TAGTGCG	60	3.6379788E-12	40.333332	1
TACGGGT	95	0.0	39.36842	4
GTTTTTT	17100	0.0	39.123978	2
ACGGGTA	175	0.0	37.714287	5
CGACGGT	135	0.0	37.48148	28
CACGACG	135	0.0	37.48148	26
GCGATAC	130	0.0	37.23077	9
GGTACGC	30	1.301265E-4	36.666664	9
AGGGCGA	1635	0.0	36.59939	6
TACGGGA	430	0.0	36.32558	4
TAGGGCG	740	0.0	36.27027	5
CAACGAC	110	0.0	36.000004	12
AGGGCGC	485	0.0	35.835052	6
TTACGGG	335	0.0	35.46269	3
ACGGGAT	385	0.0	35.42857	5
TAGGGTA	660	0.0	35.333332	5
GCGCGAC	735	0.0	35.319725	9
>>END_MODULE