FastQCFastQC Report
Thu 26 May 2016
SRR1547227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547227_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2817053
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440821.564826788846358No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT31320.11118001684739336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT337450.043.282861
TCACGAC3900.041.7435925
CGGTCTA3950.041.2151931
CTCACGA4000.040.69999724
CGACGGT3950.040.6582328
GTTTTTT376850.039.1137052
TCGTAAG800.038.51
ACGGTCT4400.037.030
GACCGAT15100.036.5695349
TAGGGAC25600.036.5234385
GCGCGAC5550.036.4684689
TACGGGA8100.036.123464
GTTAGCG1650.036.01
ATAGGGA36250.035.9889684
AGGGCGA29800.035.9530226
ACGGGTA3550.035.943665
TAGGGAT35400.035.9209065
AGGGATT37500.035.9046
ACGGGAT9900.035.777785
CACGACG4500.035.6888926
Produced by FastQC (version 0.11.5)